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01_quality_assessment/scRNA_QC.qmd

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# QC metrics: raw data {.tabset}
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# QC metrics: raw data
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In this section, we review quality control (QC) metrics for the **raw feature matrices** generated by `Cellranger`. Only a low level filter excluding cells with <100 nUMIs (= number of unique molecular identifiers, or sequenced reads per cell) was applied when uploading the data into `R`.
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:::{.panel-tabset}
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## Cells per sample
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```{r cells raw}
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theme(plot.title = element_text(hjust = 0.5, face = "bold"))
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```
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:::
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# QC metrics: Filtered data {.tabset}
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# QC metrics: Filtered data
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Based on the above QC metrics, we filtered the dataset to isolate cells passing the following thresholds: >`r nCount_RNA_cutoff` UMIs, >`r nFeature_RNA_cutoff` genes, <`r mitoRatio_cutoff` mitochondrial gene ratio, and >`r Log10GenesPerUMI_cutoff` complexity.
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In this section, we review QC metrics for our filtered dataset.
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:::{.panel-tabset}
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## Cells per sample
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```{r cells filtered}
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theme(plot.title = element_text(hjust = 0.5, face = "bold"))
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:::
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# R session
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