diff --git a/src/modality-specific-files/microscopy.md b/src/modality-specific-files/microscopy.md
index 617407cd0c..e27f862049 100644
--- a/src/modality-specific-files/microscopy.md
+++ b/src/modality-specific-files/microscopy.md
@@ -28,7 +28,7 @@ and a guide for using macros can be found at
  https://github.com/bids-standard/bids-specification/blob/master/macros_doc.md
 -->
 {{ MACROS___make_filename_template("raw", datatypes=["micr"], suffixes=["TEM", "SEM", "uCT", "BF", "DF",
-"PC", "DIC", "FLUO", "CONF", "PLI", "CARS", "2PE", "MPE", "SR", "NLO", "OCT", "SPIM", "XPCT"], n_dupes_to_combine=4) }}
+"PC", "DIC", "FLUO", "CONF", "PLI", "CARS", "1PE", "2PE", "MPE", "SR", "NLO", "OCT", "SPIM", "XPCT"], n_dupes_to_combine=4) }}
 
 Microscopy data MUST be stored in the `micr` directory.
 
@@ -82,6 +82,7 @@ and a guide for using macros can be found at
          "CONF",
          "PLI",
          "CARS",
+         "1PE",
          "2PE",
          "MPE",
          "SR",
diff --git a/src/schema/objects/suffixes.yaml b/src/schema/objects/suffixes.yaml
index 1e2825ced2..2d54d9a1e4 100644
--- a/src/schema/objects/suffixes.yaml
+++ b/src/schema/objects/suffixes.yaml
@@ -1,6 +1,11 @@
 ---
 # This file defines valid BIDS file suffixes.
 # For rules regarding how suffixes relate to datatypes, see files in `rules/files/raw/`.
+OnePE:
+  value: 1PE
+  display_name: 1-photon excitation microscopy
+  description: |
+    1-photon excitation microscopy imaging data
 TwoPE:
   value: 2PE
   display_name: 2-photon excitation microscopy
diff --git a/src/schema/rules/files/raw/micr.yaml b/src/schema/rules/files/raw/micr.yaml
index b256033ce9..8621219ad6 100644
--- a/src/schema/rules/files/raw/micr.yaml
+++ b/src/schema/rules/files/raw/micr.yaml
@@ -12,6 +12,7 @@ microscopy:
     - CONF
     - PLI
     - CARS
+    - 1PE
     - 2PE
     - MPE
     - SR