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updating 10 with pdf
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Markdowns/10_DE_analysis_with_DESeq2_part1.Rmd

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@@ -3,10 +3,10 @@ title: "Introduction to Bulk RNAseq data analysis"
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subtitle: Differential Expression of RNA-seq data - Part 1
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date: '`r format(Sys.time(), "Last modified: %d %b %Y")`'
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output:
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html_document:
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toc: yes
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pdf_document:
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toc: yes
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html_document:
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toc: yes
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bibliography: ref.bib
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---
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### Exercise 1
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> Now we have made our results table using our simple model, let have a look at which
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> genes are changing and how many pass our 0.05 threshold. WHy might this not be
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> genes are changing and how many pass our 0.05 threshold. Why might this not be
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> straigtforward?
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>
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>
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```{r echo=FALSE, message=FALSE, include=FALSE}
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sum(results.simple$padj < 0.05)
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```
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> a) how many genes are significantly (with an FDR < 0.05) up-regulated?
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>
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>
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```{r echo=FALSE, message=FALSE, include=FALSE}
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sum(results.simple$padj < 0.05 & results.simple$log2FoldChange > 0, na.rm = TRUE)
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```
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>
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> b) how many genes are significantly (with an FDR < 0.05) down-regulated?
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>
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>
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```{r echo=FALSE, message=FALSE, include=FALSE}
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sum(results.simple$padj < 0.05 & results.simple$log2FoldChange < 0, na.rm = TRUE)
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```

Markdowns/10_DE_analysis_with_DESeq2_part1.html

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<h1 class="title toc-ignore">Introduction to Bulk RNAseq data analysis</h1>
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<h3 class="subtitle">Differential Expression of RNA-seq data - Part 1</h3>
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<h4 class="date">Last modified: 12 Nov 2021</h4>
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<h4 class="date">Last modified: 22 Nov 2021</h4>
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</div>
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<div id="exercise-1" class="section level3">
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<h3>Exercise 1</h3>
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<blockquote>
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<p>Now we have made our results table using our simple model, let have a look at which genes are changing and how many pass our 0.05 threshold. WHy might this not be straigtforward?</p>
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<p>Now we have made our results table using our simple model, let have a look at which genes are changing and how many pass our 0.05 threshold. Why might this not be straigtforward?</p>
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</blockquote>
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<blockquote>
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<ol style="list-style-type: lower-alpha">
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