Which Cells to Use for Analysis

[zoe9324]

I would like to investigate the function of gene A in tumor cells. In my single-cell transcriptomic data, gene A is expressed in only a subset of tumor cells (12% of tumor cells show expression). When performing analysis with scTenifoldKnk, should I use all tumor cells, or the tumor cells expressing gene A, or only those with high expression of gene A (further selecting the top 20% of cells with the highest expression from the gene A-expressing tumor cells)?