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README.md

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# Cell type annotation
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**HiTME's is an R package that combines [scGate](https://github.com/carmonalab/scGate) and [ProjecTILs](https://github.com/carmonalab/ProjecTILs) to classify cell types in single-cell RNA-seq data at high resolution and with great flexibility and transparency.**
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**HiTME is an R package that combines [scGate](https://github.com/carmonalab/scGate) and [ProjecTILs](https://github.com/carmonalab/ProjecTILs) to classify cell types in single-cell RNA-seq data at high resolution and with large flexibility (e.g. easy to include new cell types).**
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The function takes as input `Seurat` objects (or list of them). These should be split by sample to avoid batch effects, or split internally in `Run.HitME` by indicating the parameter `split.by`.
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3.2. frequency
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3.3. CLR (Central log ratio)-transformed frequency
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3.3. CLR (Centred log ratio)-transformed counts (useful for downstream analyses such as PCA/[Logratio analysis](https://doi.org/10.1146/annurev-statistics-042720-124436) )
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4. Aggregated profile of predicted cell types: `aggregated_profile`. Including:
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