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nextflow.config
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86 lines (66 loc) · 3.16 KB
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/*
_____ _____ _____
/\ \ /\ \ /\ \
/::\ \ /::\ \ /::\ \
/::::\ \ \:::\ \ /::::\ \
/::::::\ \ \:::\ \ /::::::\ \
/:::/\:::\ \ \:::\ \ /:::/\:::\ \
/:::/__\:::\ \ \:::\ \ /:::/__\:::\ \
\:::\ \:::\ \ /::::\ \ /::::\ \:::\ \
___\:::\ \:::\ \ ____ /::::::\ \ /::::::\ \:::\ \
/\ \:::\ \:::\ \ /\ \ /:::/\:::\ \ /:::/\:::\ \:::\____\
/::\ \:::\ \:::\____\/::\ \/:::/ \:::\____\/:::/ \:::\ \:::| |
\:::\ \:::\ \::/ /\:::\ /:::/ \::/ /\::/ \:::\ /:::|____|
\:::\ \:::\ \/____/ \:::\/:::/ / \/____/ \/_____/\:::\/:::/ /
\:::\ \:::\ \ \::::::/ / \::::::/ /
\:::\ \:::\____\ \::::/____/ \::::/ /
\:::\ /:::/ / \:::\ \ \::/____/
\:::\/:::/ / \:::\ \ ~~
\::::::/ / \:::\ \
\::::/ / \:::\____\
\::/ / \::/ /
\/____/ \/____/
__ _
/ _(_)
___ ___ _ __ | |_ _ __ _ ___
/ __/ _ \| '_ \| _| |/ _` / __|
| (_| (_) | | | | | | | (_| \__ \
\___\___/|_| |_|_| |_|\__, |___/
__/ |
|___/
*/
// basic inputs and outputs
params.cellhome = "/data/hca/chensijie/Dataset/SMART1/FASTQfilesSub"
params.output = "/data/hca/chensijie/Result/SubSMART1_Result"
// analysis step configs
params.readqc = "fastp"
params.quant = "salmon"
params.quant_unit = "count"
params.downstream = "seurat"
// notification options
params.use_email = false
params.email = "chensj16@mails.tsinghua.edu.cn"
// modifiable built-in options
/// Reference
params.salmon_index="/data/hca/chensijie/Reference/transcriptome/SALMON21_Homo_sapiens.GRCh38.cdna.all"
params.tx2gene = "/data/hca/chensijie/code/SIP/scripts/tx2gene.SALMON21_Homo_sapiens.GRCh38.cdna.all.tsv"
// Seurat Analysis Parameters
params.seuratMinCells = "1"
params.seuratMinGenes = "200"
params.seuratNGeneLowerBound = "200"
params.seuratNGeneUpperBound = "Inf"
params.seuratMitoLowerBound = "-Inf"
params.seuratMitoUpperBound = "0.30"
params.seuratSubgroupResolution = "1.03"
params.seuratTSNEPerplexity = "4"
params.seuratDEGeneKept = "10"
params.seuratDEGeneViz = "3"
params.seuratDEMinDetectRatio= "0.15"
params.seuratDEpvalCut = "0.05"
process{
container= 'sunhao2018/centos_docker_test:latest'
}
docker{
enabled=true
fixOwnership=true
}