Identifying AS events per isoform after diffSplice in SUPPA2

[Freeddows]

Hello

Im not sure if this is the right place to ask this, but I hope someone can help.

I've successfully run the diffSplice step to compare AS events (local events and transcript usage) between two conditions. Now, Id like to dig deeper into the results and identify which specific AS event(s) are associated with each differentially expressed isoform (|dPSI| > 0.1, for example).

In other words:
1 - Is there a way to trace back the AS event(s) that contributed to the observed isoform differences?
2 - Can SUPPA2 output or be combined with any tool to link isoforms with specific AS events?

Any guidance or tips on how to proceed with this kind of analysis would be greatly appreciated.

Thanks in advance!

[EduEyras]

Hi Suppa already provides this! :-) The .ioe file links the events and isoforms. The first column of isoforms ids are those that define the alternative event The second column of isoforms ids are those plus the reference isoforms for that event The first column of the ioe file is your event id There you have all the info you need I hope this helps E

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On Tue, 27 May 2025 at 06:07, Freeddows ***@***.***> wrote: *Freeddows* created an issue (comprna/SUPPA#215) <#215> Hello Im not sure if this is the right place to ask this, but I hope someone can help. I've successfully run the diffSplice step to compare AS events (local events and transcript usage) between two conditions. Now, Id like to dig deeper into the results and identify which specific AS event(s) are associated with each differentially expressed isoform (|dPSI| > 0.1, for example). In other words: 1 - Is there a way to trace back the AS event(s) that contributed to the observed isoform differences? 2 - Can SUPPA2 output or be combined with any tool to link isoforms with specific AS events? Any guidance or tips on how to proceed with this kind of analysis would be greatly appreciated. Thanks in advance! — Reply to this email directly, view it on GitHub <#215>, or unsubscribe <https://github.com/notifications/unsubscribe-auth/ADCZKB2HDAZTMKBVGG3FHU33ARPR5AVCNFSM6AAAAAB6AACAMCVHI2DSMVQWIX3LMV43ASLTON2WKOZTGA4TGOBYGA3TANI> . You are receiving this because you are subscribed to this thread.Message ID: ***@***.***>