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- By default, the applications pre-compute the image embeddings produced by SegmentAnything and store them on disc. If you are using a CPU this step can take a while for 3d data or timeseries (you will see a progress bar with a time estimate). If you have access to a GPU without graphical interface (e.g. via a local computer cluster or a cloud provider), you can also pre-compute the embeddings there and then copy them to your laptop / local machine to speed this up. You can use the command `micro_sam.precompute_embeddings` for this (it is installed with the rest of the applications). You can specify the location of the precomputed embeddings via the `embedding_path` argument.
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- Most other processing steps are very fast even on a CPU, so interactive annotation is possible. An exception is the automatic segmentation step (2d segmentation), which takes several minutes without a GPU (depending on the image size). For large volumes and timeseries segmenting an object in 3d / tracking across time can take a couple settings with a CPU (it is very fast with a GPU).
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- You can save and load the results from the `committed_objects` / `committed_tracks` layer to correct segmentations you obtained from another tool (e.g. CellPose) or to save intermediate annotation results. The results can be saved via `File->Save Selected Layer(s) ...` in the napari menu. They can be loaded again by specifying the corresponding location via the `segme` (2d and 3d segmentation) `xxx` (tracking) argument.
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- You can save and load the results from the `committed_objects` / `committed_tracks` layer to correct segmentations you obtained from another tool (e.g. CellPose) or to save intermediate annotation results. The results can be saved via `File->Save Selected Layer(s) ...` in the napari menu (see the tutorial videos for details). They can be loaded again by specifying the corresponding location via the `segmentation_result` (2d and 3d segmentation) or `tracking_result` (tracking) argument.
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