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---
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- title : " Signal and batch correction for mass spectrometry"
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+ title : " Signal drift and batch effect correction for mass spectrometry"
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author :
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name : " Andris Jankevics"
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affiliation : Phenome Centre Birmingham, University of Birmingham
@@ -16,7 +16,7 @@ output:
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bibliography : pmp.bib
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vignette : >
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- %\VignetteIndexEntry{Signal and batch correction for mass spectrometry}
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+ %\VignetteIndexEntry{Signal drift and batch effect correction for mass spectrometry}
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%\VignetteEngine{knitr::rmarkdown}
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%\VignetteEncoding{UTF-8}
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---
@@ -35,8 +35,8 @@ Correction (QC-RSC) [@kirwan2013] algorithm for signal drift and batch effect
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correction within/across a multi-batch direct infusion mass spectrometry (DIMS)
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and liquid chromatography mass spectrometry (LCMS) datasets.
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- Please read "Signal and batch correction, data assessment and
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- correction " vignette to learn how to assess your dataset and details on
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+ Please read "Signal drift and batch effect correction and mass spectral quality
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+ assessment " vignette to learn how to assess your dataset and details on
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algorithm itself.
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# Installation
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# Dataset
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- In this tutorial we will be using an direct infusion mass spectrometry (DIMS)
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+ In this tutorial we will be using a direct infusion mass spectrometry (DIMS)
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dataset consisting of 172 samples measured across 8 batches and is included in
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` pmp ` package as ` SummarizedExperiemnt ` class object ` MTBLS79 ` .
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More detailed description of the dataset is available from @kirwan2014 ,
@@ -140,11 +140,13 @@ plots
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The scores plots of principal components analysis (PCA) before
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and after correction can be used to asses effects of data correction.
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- In this example, ` PQN ` method is used to normalise data,
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- ` KNN ` for missing value imputation and ` glog ` for data scaling. All functions
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- are availiable as a part of ` r Biocpkg("pmp") ` package.
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+ In this example, probabilistic quotient normalisation (` PQN ` ) method is used to
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+ normalise data, k-nearest neighbours (` KNN ` ) for missing value imputation and
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+ ` glog ` for data scaling. All functions are availiable as a part of
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+ ` r Biocpkg("pmp") ` package.
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- A more detailed overview on data pre-processing is detailed in @guida2016 .
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+ See @guida2016 for a more detailed review on common pre-processing steps and
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+ methods.
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``` {r, fig.width=6, fig.height=8}
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