@@ -26,25 +26,25 @@ execute:
2626
2727## Schedule Overview
2828
29- Today, 20.01.2026
30- 11:00 - 12:00 - Set up your computers
31- 12:00 - 13:00 - Lunch Break
32- 13:00 - 14:30 - Loading Xenium data into ` R ` and ` Seurat `
33- 14:30 - 14:50 - Coffee Break
34- 14:50 - 15:00 - Group Photo
35- 15:00 - 17:00 - Spatial Transcriptomics Data Analysis
29+ Today, 20.01.2026 \
30+ 11:00 - 12:00 - Set up your computers \
31+ 12:00 - 13:00 - Lunch Break \
32+ 13:00 - 14:30 - Loading Xenium data into ` R ` and ` Seurat ` \
33+ 14:30 - 14:50 - Coffee Break \
34+ 14:50 - 15:00 - Group Photo \
35+ 15:00 - 17:00 - Spatial Transcriptomics Data Analysis \
3636
3737  ;
3838
39- Tomorrow, 21.01.2026
40- 09:00 - 10:20 - Continuation Data Analysis
41- 10:20 - 10:40 - Coffee Break
42- 10:40 - 12:00 - Continuation Data Analysis
39+ Tomorrow, 21.01.2026 \
40+ 09:00 - 10:20 - Continuation Data Analysis \
41+ 10:20 - 10:40 - Coffee Break \
42+ 10:40 - 12:00 - Continuation Data Analysis \
4343
4444## Code Availability
4545
46- After the training, you will be able to access all materials via our GitHub repository:
47- https://github.com/dcgc-bfx/spatial-analysis-xenium-training
46+ After the training, you will be able to access all materials via our GitHub repository: \
47+ < https://github.com/dcgc-bfx/spatial-analysis-xenium-training >
4848
4949:::: {.columns}
5050
@@ -62,7 +62,7 @@ You are welcome to contribute and develop the code further!
6262
6363## Netiquette
6464
65- We prepared code - let us run it in sync with each other
65+ We prepared code - let us run it in sync with each other \
6666(do not run your code ahead of the trainer)
6767
6868  ;
@@ -71,24 +71,88 @@ Ask questions - we are here to help you!
7171
7272## LinkedIn
7373
74- Follow us on LinkedIn for updates on future trainings and events!
75- https://www.linkedin.com/company/dresden-concept-genome-center/
74+ Follow us on LinkedIn for updates on future trainings and events! \
75+ < https://www.linkedin.com/company/dresden-concept-genome-center/ >
7676![ ] ( ../docs/DcGC_LinkedIn.png ) {width=80%}
7777
7878  ;
7979
80- https://www.linkedin.com/in/katrinsameith/
81- https://www.linkedin.com/in/ulrike-anne-friedrich-7940ab142/
82- https://www.linkedin.com/in/rajinder-gupta-11512751/
80+ < https://www.linkedin.com/in/katrinsameith/ > \
81+ < https://www.linkedin.com/in/ulrike-anne-friedrich-7940ab142/ > \
82+ < https://www.linkedin.com/in/rajinder-gupta-11512751/ > \
8383
8484## Data Analysis Cafe
8585
86- Feel free to reach out to us for follow-up questions, and make use of our monthly Data Analysis Cafe:
86+ Feel free to reach out to us for follow-up questions, and make use of our monthly Data Analysis Cafe: \
8787![ ] ( ../docs/DcGC_DataAnalysisCafe_lowres.png ) {width=35%}
8888
89- ## Spatial Analysis Flowchart
89+ ## Literature and toolkits to get started
90+
91+ Best practices: \
92+ - Single-cell best practices: < https://www.sc-best-practices.org/preamble.html > \
93+
94+   ;
95+
96+ Seurat: \
97+ - Seurat Xenium Analysis Vignette: < https://satijalab.org/seurat/articles/seurat5_spatial_vignette_2 > \
98+
99+   ;
100+
101+ Alternatives to Seurat \
102+ - Squidpy: < https://squidpy.readthedocs.io/en/stable/ > \
103+ - Giotto: < https://giottosuite.readthedocs.io/en/latest/ > \
104+
105+ ## Seurat vignettes are a good way to get started
106+
107+ ![ ] ( ../docs/Seurat_Vignettes.png )
108+
109+ ## Spatial Analysis Overview
90110
91111![ ] ( ../docs/SpatialAnalysisFlowchart.png )
92112
113+ ## Clean-up
114+
115+ - If you would like to keep your copies of the workbook material: \
116+ - Please copy it now \
117+ - The files are in your home directory on the HPC cluster \
118+ - Do not copy the dataset files in the ` datasets ` directory. Instead, copy only the ` download.R ` file and use it for download \
119+
120+   ;
121+
122+ - RStudio server jobs will be stopped automatically
123+
124+ ## How to use the code later on
125+
126+ All workshop material is available online at: \
127+ < https://github.com/dcgc-bfx/spatial-analysis-xenium-training >
128+
129+   ;
93130
131+ On a linux machine, you can use our singularity container
132+
133+ ```
134+ # Command to download the singularity container
135+ singularity pull oras://gcr.hrz.tu-chemnitz.de/dcgc-bfx/singularity/singularity-single-cell:v1.6.6
136+ ```
137+
138+   ;
139+
140+ On Windows or MacOS, you can install R and RStudio locally and install the required packages
141+
142+ ## LinkedIn
143+
144+ Follow us on LinkedIn for updates on future trainings and events! \
145+ < https://www.linkedin.com/company/dresden-concept-genome-center/ >
146+ ![ ] ( ../docs/DcGC_LinkedIn.png ) {width=80%}
147+
148+   ;
149+
150+ < https://www.linkedin.com/in/katrinsameith/ > \
151+ < https://www.linkedin.com/in/ulrike-anne-friedrich-7940ab142/ > \
152+ < https://www.linkedin.com/in/rajinder-gupta-11512751/ > \
153+
154+ ## Data Analysis Cafe
155+
156+ Feel free to reach out to us for follow-up questions, and make use of our monthly Data Analysis Cafe: \
157+ ![ ] ( ../docs/DcGC_DataAnalysisCafe_lowres.png ) {width=35%}
94158
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