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Updated presentation slides
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## Schedule Overview
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Today, 20.01.2026
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11:00 - 12:00 - Set up your computers
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12:00 - 13:00 - Lunch Break
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13:00 - 14:30 - Loading Xenium data into `R` and `Seurat`
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14:30 - 14:50 - Coffee Break
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14:50 - 15:00 - Group Photo
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15:00 - 17:00 - Spatial Transcriptomics Data Analysis
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Today, 20.01.2026 \
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11:00 - 12:00 - Set up your computers \
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12:00 - 13:00 - Lunch Break \
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13:00 - 14:30 - Loading Xenium data into `R` and `Seurat` \
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14:30 - 14:50 - Coffee Break \
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14:50 - 15:00 - Group Photo \
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15:00 - 17:00 - Spatial Transcriptomics Data Analysis \
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Tomorrow, 21.01.2026
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09:00 - 10:20 - Continuation Data Analysis
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10:20 - 10:40 - Coffee Break
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10:40 - 12:00 - Continuation Data Analysis
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Tomorrow, 21.01.2026 \
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09:00 - 10:20 - Continuation Data Analysis \
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10:20 - 10:40 - Coffee Break \
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10:40 - 12:00 - Continuation Data Analysis \
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## Code Availability
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After the training, you will be able to access all materials via our GitHub repository:
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https://github.com/dcgc-bfx/spatial-analysis-xenium-training
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After the training, you will be able to access all materials via our GitHub repository: \
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<https://github.com/dcgc-bfx/spatial-analysis-xenium-training>
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:::: {.columns}
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## Netiquette
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We prepared code - let us run it in sync with each other
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We prepared code - let us run it in sync with each other \
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(do not run your code ahead of the trainer)
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&nbsp;
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## LinkedIn
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Follow us on LinkedIn for updates on future trainings and events!
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https://www.linkedin.com/company/dresden-concept-genome-center/
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Follow us on LinkedIn for updates on future trainings and events! \
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<https://www.linkedin.com/company/dresden-concept-genome-center/>
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![](../docs/DcGC_LinkedIn.png){width=80%}
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&nbsp;
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https://www.linkedin.com/in/katrinsameith/
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https://www.linkedin.com/in/ulrike-anne-friedrich-7940ab142/
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https://www.linkedin.com/in/rajinder-gupta-11512751/
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<https://www.linkedin.com/in/katrinsameith/> \
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<https://www.linkedin.com/in/ulrike-anne-friedrich-7940ab142/> \
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<https://www.linkedin.com/in/rajinder-gupta-11512751/> \
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## Data Analysis Cafe
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Feel free to reach out to us for follow-up questions, and make use of our monthly Data Analysis Cafe:
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Feel free to reach out to us for follow-up questions, and make use of our monthly Data Analysis Cafe: \
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![](../docs/DcGC_DataAnalysisCafe_lowres.png){width=35%}
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## Spatial Analysis Flowchart
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## Literature and toolkits to get started
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Best practices: \
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- Single-cell best practices: <https://www.sc-best-practices.org/preamble.html> \
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&nbsp;
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Seurat: \
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- Seurat Xenium Analysis Vignette: <https://satijalab.org/seurat/articles/seurat5_spatial_vignette_2> \
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&nbsp;
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Alternatives to Seurat \
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- Squidpy: <https://squidpy.readthedocs.io/en/stable/> \
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- Giotto: <https://giottosuite.readthedocs.io/en/latest/> \
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## Seurat vignettes are a good way to get started
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![](../docs/Seurat_Vignettes.png)
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## Spatial Analysis Overview
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![](../docs/SpatialAnalysisFlowchart.png)
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## Clean-up
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- If you would like to keep your copies of the workbook material: \
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- Please copy it now \
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- The files are in your home directory on the HPC cluster \
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- Do not copy the dataset files in the `datasets` directory. Instead, copy only the `download.R` file and use it for download \
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&nbsp;
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- RStudio server jobs will be stopped automatically
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## How to use the code later on
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All workshop material is available online at: \
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<https://github.com/dcgc-bfx/spatial-analysis-xenium-training>
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&nbsp;
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On a linux machine, you can use our singularity container
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```
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# Command to download the singularity container
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singularity pull oras://gcr.hrz.tu-chemnitz.de/dcgc-bfx/singularity/singularity-single-cell:v1.6.6
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```
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&nbsp;
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On Windows or MacOS, you can install R and RStudio locally and install the required packages
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## LinkedIn
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Follow us on LinkedIn for updates on future trainings and events! \
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<https://www.linkedin.com/company/dresden-concept-genome-center/>
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![](../docs/DcGC_LinkedIn.png){width=80%}
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&nbsp;
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<https://www.linkedin.com/in/katrinsameith/> \
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<https://www.linkedin.com/in/ulrike-anne-friedrich-7940ab142/> \
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<https://www.linkedin.com/in/rajinder-gupta-11512751/> \
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## Data Analysis Cafe
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Feel free to reach out to us for follow-up questions, and make use of our monthly Data Analysis Cafe: \
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![](../docs/DcGC_DataAnalysisCafe_lowres.png){width=35%}
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