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Hi deeptools team, thank you for having developed such good tools.
The problem I met is that, when combining "computeMatrix scale-regions" and "plotHeatmap", I alwasy see blank regions in the plot (see below). I don't know how it was generated and how to avoid it. But when using "computeMatrix reference-point", there is no such suitation.
Here is the code I used
samtools index $indir/K4me1.bam
bamCoverage -b $indir/K4me1.bam -o $outdir/K4me1.bw -bs 50 -p 30
computeMatrix scale-regions \
-a 5000 \
-b 5000 \
-S $outdir/K4me1.bw \
-R Merged_K562.bed \
-o $outdir/K4me1_Region.gz \
-p 20 \
--skipZeros \
--missingDataAsZero
plotHeatmap \
-m $outdir/K4me1_Region.gz \
-o $outdir/2025-07-04_K4me1_Heatmap.pdf \
--colorList 'white,#2600f6' \
--samplesLabel K4me1_G15-16 \
--zMax auto \
--refPointLabel PeakCenter \
--startLabel Start \
--endLabel End

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