diff --git a/src/Components/Web.JS/src/GlobalExports.ts b/src/Components/Web.JS/src/GlobalExports.ts
index d5f3128d424a..a62996e70fb3 100644
--- a/src/Components/Web.JS/src/GlobalExports.ts
+++ b/src/Components/Web.JS/src/GlobalExports.ts
@@ -74,6 +74,7 @@ export interface IBlazor {
     renderBatch?: (browserRendererId: number, batchAddress: Pointer) => void;
     getConfig?: (fileName: string) => Uint8Array | undefined;
     getApplicationEnvironment?: () => string;
+    getApplicationCulture?: () => string;
     dotNetCriticalError?: any;
     loadLazyAssembly?: any;
     loadSatelliteAssemblies?: any;
diff --git a/src/Components/Web.JS/src/Platform/Mono/MonoPlatform.ts b/src/Components/Web.JS/src/Platform/Mono/MonoPlatform.ts
index 1465e5f79cc8..efb8cb7d233b 100644
--- a/src/Components/Web.JS/src/Platform/Mono/MonoPlatform.ts
+++ b/src/Components/Web.JS/src/Platform/Mono/MonoPlatform.ts
@@ -154,6 +154,7 @@ function prepareRuntimeConfig(options: Partial<WebAssemblyStartOptions>, onConfi
     }
 
     Blazor._internal.getApplicationEnvironment = () => loadedConfig.applicationEnvironment!;
+    Blazor._internal.getApplicationCulture = () => loadedConfig.applicationCulture!;
 
     onConfigLoadedCallback?.(loadedConfig);
 
diff --git a/src/Components/WebAssembly/WebAssembly/src/Hosting/WebAssemblyCultureProvider.cs b/src/Components/WebAssembly/WebAssembly/src/Hosting/WebAssemblyCultureProvider.cs
index 99d1476dc10d..4d9f51634da0 100644
--- a/src/Components/WebAssembly/WebAssembly/src/Hosting/WebAssemblyCultureProvider.cs
+++ b/src/Components/WebAssembly/WebAssembly/src/Hosting/WebAssemblyCultureProvider.cs
@@ -17,23 +17,19 @@ internal partial class WebAssemblyCultureProvider
     internal const string ReadSatelliteAssemblies = "window.Blazor._internal.readSatelliteAssemblies";
 
     // For unit testing.
-    internal WebAssemblyCultureProvider(CultureInfo initialCulture, CultureInfo initialUICulture)
+    internal WebAssemblyCultureProvider(CultureInfo initialCulture)
     {
         InitialCulture = initialCulture;
-        InitialUICulture = initialUICulture;
     }
 
     public static WebAssemblyCultureProvider? Instance { get; private set; }
 
     public CultureInfo InitialCulture { get; }
 
-    public CultureInfo InitialUICulture { get; }
-
     internal static void Initialize()
     {
         Instance = new WebAssemblyCultureProvider(
-            initialCulture: CultureInfo.CurrentCulture,
-            initialUICulture: CultureInfo.CurrentUICulture);
+            initialCulture: CultureInfo.GetCultureInfo(WebAssemblyCultureProviderInterop.GetApplicationCulture() ?? CultureInfo.InvariantCulture.Name));
     }
 
     public void ThrowIfCultureChangeIsUnsupported()
@@ -48,8 +44,7 @@ public void ThrowIfCultureChangeIsUnsupported()
         // The current method is invoked as part of WebAssemblyHost.RunAsync i.e. after user code in Program.MainAsync has run
         // thus allows us to detect if the culture was changed by user code.
         if (Environment.GetEnvironmentVariable("__BLAZOR_SHARDED_ICU") == "1" &&
-            ((!CultureInfo.CurrentCulture.Name.Equals(InitialCulture.Name, StringComparison.Ordinal) ||
-              !CultureInfo.CurrentUICulture.Name.Equals(InitialUICulture.Name, StringComparison.Ordinal))))
+            (!CultureInfo.CurrentCulture.Name.Equals(InitialCulture.Name, StringComparison.Ordinal)))
         {
             throw new InvalidOperationException("Blazor detected a change in the application's culture that is not supported with the current project configuration. " +
                 "To change culture dynamically during startup, set <BlazorWebAssemblyLoadAllGlobalizationData>true</BlazorWebAssemblyLoadAllGlobalizationData> in the application's project file.");
@@ -118,5 +113,8 @@ private partial class WebAssemblyCultureProviderInterop
     {
         [JSImport("INTERNAL.loadSatelliteAssemblies")]
         public static partial Task LoadSatelliteAssemblies(string[] culturesToLoad);
+
+        [JSImport("Blazor._internal.getApplicationCulture", "blazor-internal")]
+        public static partial string GetApplicationCulture();
     }
 }
diff --git a/src/Components/WebAssembly/WebAssembly/src/Services/IInternalJSImportMethods.cs b/src/Components/WebAssembly/WebAssembly/src/Services/IInternalJSImportMethods.cs
index 83f14d1bd2b1..560f64fa1154 100644
--- a/src/Components/WebAssembly/WebAssembly/src/Services/IInternalJSImportMethods.cs
+++ b/src/Components/WebAssembly/WebAssembly/src/Services/IInternalJSImportMethods.cs
@@ -8,6 +8,8 @@ internal interface IInternalJSImportMethods
     string GetPersistedState();
 
     string GetApplicationEnvironment();
+    
+    string GetApplicationCulture();
 
     void AttachRootComponentToElement(string domElementSelector, int componentId, int rendererId);
 
diff --git a/src/Components/WebAssembly/WebAssembly/src/Services/InternalJSImportMethods.cs b/src/Components/WebAssembly/WebAssembly/src/Services/InternalJSImportMethods.cs
index 811a78dbc652..628d1c4f7186 100644
--- a/src/Components/WebAssembly/WebAssembly/src/Services/InternalJSImportMethods.cs
+++ b/src/Components/WebAssembly/WebAssembly/src/Services/InternalJSImportMethods.cs
@@ -27,6 +27,9 @@ public static async Task<RootComponentOperationBatch> GetInitialComponentUpdate(
     public string GetApplicationEnvironment()
         => GetApplicationEnvironmentCore();
 
+    public string GetApplicationCulture()
+        => GetApplicationCultureCore();
+
     public void AttachRootComponentToElement(string domElementSelector, int componentId, int rendererId)
         => AttachRootComponentToElementCore(domElementSelector, componentId, rendererId);
 
@@ -72,6 +75,9 @@ public string RegisteredComponents_GetParameterValues(int id)
     [JSImport("Blazor._internal.getApplicationEnvironment", "blazor-internal")]
     private static partial string GetApplicationEnvironmentCore();
 
+    [JSImport("Blazor._internal.getApplicationCulture", "blazor-internal")]
+    private static partial string GetApplicationCultureCore();
+
     [JSImport("Blazor._internal.attachRootComponentToElement", "blazor-internal")]
     private static partial void AttachRootComponentToElementCore(string domElementSelector, int componentId, int rendererId);
 
diff --git a/src/Components/WebAssembly/WebAssembly/test/Hosting/WebAssemblyCultureProviderTest.cs b/src/Components/WebAssembly/WebAssembly/test/Hosting/WebAssemblyCultureProviderTest.cs
index 22ad7d18fffc..2406a919b2f6 100644
--- a/src/Components/WebAssembly/WebAssembly/test/Hosting/WebAssemblyCultureProviderTest.cs
+++ b/src/Components/WebAssembly/WebAssembly/test/Hosting/WebAssemblyCultureProviderTest.cs
@@ -47,7 +47,7 @@ public void ThrowIfCultureChangeIsUnsupported_ThrowsIfCulturesAreDifferentAndICU
         try
         {
             // WebAssembly is initialized with en-US
-            var cultureProvider = new WebAssemblyCultureProvider(new CultureInfo("en-US"), new CultureInfo("en-US"));
+            var cultureProvider = new WebAssemblyCultureProvider(new CultureInfo("en-US"));
 
             // Culture is changed to fr-FR as part of the app
             using var cultureReplacer = new CultureReplacer("fr-FR");
diff --git a/src/Components/WebAssembly/WebAssembly/test/Hosting/WebAssemblyHostTest.cs b/src/Components/WebAssembly/WebAssembly/test/Hosting/WebAssemblyHostTest.cs
index 552ca8272707..2083c8230b1b 100644
--- a/src/Components/WebAssembly/WebAssembly/test/Hosting/WebAssemblyHostTest.cs
+++ b/src/Components/WebAssembly/WebAssembly/test/Hosting/WebAssemblyHostTest.cs
@@ -97,7 +97,7 @@ public ValueTask DisposeAsync()
     private class TestSatelliteResourcesLoader : WebAssemblyCultureProvider
     {
         internal TestSatelliteResourcesLoader()
-            : base(CultureInfo.CurrentCulture, CultureInfo.CurrentUICulture)
+            : base(CultureInfo.CurrentCulture)
         {
         }
 
diff --git a/src/Components/WebAssembly/WebAssembly/test/TestInternalJSImportMethods.cs b/src/Components/WebAssembly/WebAssembly/test/TestInternalJSImportMethods.cs
index 74e1d6b676ac..9aa4ebd48f62 100644
--- a/src/Components/WebAssembly/WebAssembly/test/TestInternalJSImportMethods.cs
+++ b/src/Components/WebAssembly/WebAssembly/test/TestInternalJSImportMethods.cs
@@ -16,6 +16,9 @@ public TestInternalJSImportMethods(string environment = "Production")
 
     public string GetApplicationEnvironment()
         => _environment;
+    
+    public string GetApplicationCulture()
+        => "en-US";
 
     public string GetPersistedState()
         => null;