diff --git a/instrumentation/opentelemetry-instrumentation-fastapi/test-requirements.txt b/instrumentation/opentelemetry-instrumentation-fastapi/test-requirements.txt
index 0aab3a13e4..c3adbef79a 100644
--- a/instrumentation/opentelemetry-instrumentation-fastapi/test-requirements.txt
+++ b/instrumentation/opentelemetry-instrumentation-fastapi/test-requirements.txt
@@ -19,7 +19,7 @@ pydantic_core==2.20.1
 pytest==7.4.4
 requests==2.32.3
 sniffio==1.3.0
-starlette==0.36.3
+starlette==0.40.0
 tomli==2.0.1
 typing_extensions==4.12.2
 urllib3==2.2.2
diff --git a/instrumentation/opentelemetry-instrumentation-starlette/pyproject.toml b/instrumentation/opentelemetry-instrumentation-starlette/pyproject.toml
index ef8e8fd5f5..7f27777469 100644
--- a/instrumentation/opentelemetry-instrumentation-starlette/pyproject.toml
+++ b/instrumentation/opentelemetry-instrumentation-starlette/pyproject.toml
@@ -34,7 +34,7 @@ dependencies = [
 ]
 
 [project.optional-dependencies]
-instruments = ["starlette >= 0.13, <0.15"]
+instruments = ["starlette >=0.40.0, <0.41"]
 
 [project.entry-points.opentelemetry_instrumentor]
 starlette = "opentelemetry.instrumentation.starlette:StarletteInstrumentor"
diff --git a/instrumentation/opentelemetry-instrumentation-starlette/test-requirements.latest.txt b/instrumentation/opentelemetry-instrumentation-starlette/test-requirements.latest.txt
index 7b6dd8cff4..e99f13cac6 100644
--- a/instrumentation/opentelemetry-instrumentation-starlette/test-requirements.latest.txt
+++ b/instrumentation/opentelemetry-instrumentation-starlette/test-requirements.latest.txt
@@ -72,7 +72,7 @@ requests==2.32.3
     #   -r instrumentation/opentelemetry-instrumentation-starlette/test-requirements.in
 sniffio==1.3.1
     # via anyio
-starlette==0.14.2
+starlette==0.40.0
     # via opentelemetry-instrumentation-starlette
 tomli==2.2.1 ; python_full_version < '3.11'
     # via pytest
diff --git a/instrumentation/opentelemetry-instrumentation-starlette/test-requirements.oldest.txt b/instrumentation/opentelemetry-instrumentation-starlette/test-requirements.oldest.txt
index 5f84cdf28f..2d6bbd84e8 100644
--- a/instrumentation/opentelemetry-instrumentation-starlette/test-requirements.oldest.txt
+++ b/instrumentation/opentelemetry-instrumentation-starlette/test-requirements.oldest.txt
@@ -72,7 +72,7 @@ requests==2.32.3
     #   -r instrumentation/opentelemetry-instrumentation-starlette/test-requirements.in
 sniffio==1.3.1
     # via anyio
-starlette==0.13.8
+starlette==0.40.0
     # via opentelemetry-instrumentation-starlette
 tomli==2.2.1 ; python_full_version < '3.11'
     # via pytest