diff --git a/instrumentation/opentelemetry-instrumentation-boto/test-requirements.txt b/instrumentation/opentelemetry-instrumentation-boto/test-requirements.txt
index d73e7447ed..713c9d1d4e 100644
--- a/instrumentation/opentelemetry-instrumentation-boto/test-requirements.txt
+++ b/instrumentation/opentelemetry-instrumentation-boto/test-requirements.txt
@@ -5,7 +5,7 @@ botocore==1.34.44
 certifi==2024.7.4
 cffi==1.17.0
 charset-normalizer==3.3.2
-cryptography==43.0.1
+cryptography==44.0.1
 Deprecated==1.2.14
 docker==7.0.0
 idna==3.7
diff --git a/instrumentation/opentelemetry-instrumentation-botocore/test-requirements-0.txt b/instrumentation/opentelemetry-instrumentation-botocore/test-requirements-0.txt
index 34442c9202..76688d67aa 100644
--- a/instrumentation/opentelemetry-instrumentation-botocore/test-requirements-0.txt
+++ b/instrumentation/opentelemetry-instrumentation-botocore/test-requirements-0.txt
@@ -5,7 +5,7 @@ botocore==1.31.80
 certifi==2024.7.4
 cffi==1.17.0
 charset-normalizer==3.3.2
-cryptography==43.0.1
+cryptography==44.0.1
 Deprecated==1.2.14
 docker==7.0.0
 idna==3.7
diff --git a/instrumentation/opentelemetry-instrumentation-botocore/test-requirements-1.txt b/instrumentation/opentelemetry-instrumentation-botocore/test-requirements-1.txt
index a2fa776548..0d9ca92b79 100644
--- a/instrumentation/opentelemetry-instrumentation-botocore/test-requirements-1.txt
+++ b/instrumentation/opentelemetry-instrumentation-botocore/test-requirements-1.txt
@@ -5,7 +5,7 @@ botocore==1.35.56
 certifi==2024.7.4
 cffi==1.17.0
 charset-normalizer==3.3.2
-cryptography==43.0.1
+cryptography==44.0.1
 Deprecated==1.2.14
 docker==7.0.0
 idna==3.7