diff --git a/instrumentation/opentelemetry-instrumentation-boto/test-requirements.txt b/instrumentation/opentelemetry-instrumentation-boto/test-requirements.txt
index d73e7447ed..75123db3af 100644
--- a/instrumentation/opentelemetry-instrumentation-boto/test-requirements.txt
+++ b/instrumentation/opentelemetry-instrumentation-boto/test-requirements.txt
@@ -29,7 +29,7 @@ six==1.16.0
 tomli==2.0.1
 typing_extensions==4.12.2
 urllib3==1.26.19
-Werkzeug==2.3.8
+Werkzeug==3.0.6
 wrapt==1.16.0
 xmltodict==0.13.0
 zipp==3.19.2
diff --git a/instrumentation/opentelemetry-instrumentation-flask/test-requirements-0.txt b/instrumentation/opentelemetry-instrumentation-flask/test-requirements-0.txt
index 6bebbe414a..cf0f87e5a7 100644
--- a/instrumentation/opentelemetry-instrumentation-flask/test-requirements-0.txt
+++ b/instrumentation/opentelemetry-instrumentation-flask/test-requirements-0.txt
@@ -12,7 +12,7 @@ py-cpuinfo==9.0.0
 pytest==7.4.4
 tomli==2.0.1
 typing_extensions==4.12.2
-Werkzeug==2.3.8
+Werkzeug==3.0.6
 wrapt==1.16.0
 zipp==3.19.2
 -e opentelemetry-instrumentation
diff --git a/instrumentation/opentelemetry-instrumentation-flask/test-requirements-1.txt b/instrumentation/opentelemetry-instrumentation-flask/test-requirements-1.txt
index c66c55df1d..6d453e21cb 100644
--- a/instrumentation/opentelemetry-instrumentation-flask/test-requirements-1.txt
+++ b/instrumentation/opentelemetry-instrumentation-flask/test-requirements-1.txt
@@ -12,7 +12,7 @@ py-cpuinfo==9.0.0
 pytest==7.4.4
 tomli==2.0.1
 typing_extensions==4.12.2
-Werkzeug==2.3.8
+Werkzeug==3.0.6
 wrapt==1.16.0
 zipp==3.19.2
 -e opentelemetry-instrumentation