Skip to content

Commit 19d7c2f

Browse files
authored
Update README.md
1 parent a01d982 commit 19d7c2f

File tree

1 file changed

+6
-2
lines changed

1 file changed

+6
-2
lines changed

README.md

Lines changed: 6 additions & 2 deletions
Original file line numberDiff line numberDiff line change
@@ -5,7 +5,7 @@ From r**A**w (unaligned) BAM files to normali**Z**ed counts.
55

66
A Snakemake implementation of the [BSF's](https://www.biomedical-sequencing.org/) [ATAC-seq Data Processing Pipeline](https://github.com/berguner/atacseq_pipeline "ATAC-seq Data Processing Pipeline") extended by downstream processing and unsupervised analyses steps using bash, python and R. Reproducibility and Scalability is ensured by using Snakemake, conda and Singularity.
77

8-
**If you use this workflow in a publication, don't forget to give credits to the authors by citing the URL of this (original) repository and its DOI (see Zenodo badge above).**
8+
**If you use this workflow in a publication, please don't forget to give credits to the authors by citing it using this DOI [10.5281/zenodo.6323634](https://doi.org/10.5281/zenodo.6323634).**
99

1010
![Workflow Rulegraph](./workflow/dags/atacseq_pipeline_rulegraph.svg)
1111

@@ -92,7 +92,7 @@ Trimmed mean of M-values (TMM) normalization (ver) [ref] was performed on the fi
9292
**Unsupervised Analysis & Visualization.**
9393
We applied both linear and non-linear unsupervised dimensionality reduction methods to normalized data to visualize emerging sample-wise patterns in two dimensions. We used Principal Component Analysis (PCA) [ref] from scikit-learn (ver) [ref] as the linear approach and Uniform Manifold Approximation projection (UMAP) from umap-learn (ver) [ref] with the correlation metric as the non-linear approach. For visualization matplotlib (ver) [ref] was used.
9494

95-
The processing and analysis described here was performed using a publicly available Snakemake [ver] (ref) workflow [ref - cite this workflow here].
95+
The processing and analysis described here was performed using a publicly available Snakemake [ver] (ref) workflow [[10.5281/zenodo.6323634](https://doi.org/10.5281/zenodo.6323634)].
9696

9797
# Features
9898
- Processing
@@ -246,3 +246,7 @@ provided for both test examples in workflow/dags
246246
- [GitHub Page](https://epigen.github.io/atacseq_pipeline/)
247247
- [Zenodo Repository](https://doi.org/10.5281/zenodo.6323634)
248248
- [Snakemake Workflow Catalog Entry](https://snakemake.github.io/snakemake-workflow-catalog?usage=epigen/atacseq_pipeline)
249+
250+
# Publications
251+
The following publications successfully used this module for their analyses.
252+
- [Casteels et al (2022) Cell Reports - SMNDC1 links chromatin remodeling and splicing to regulate pancreatic hormone expression.](https://doi.org/10.1016/j.celrep.2022.111288)

0 commit comments

Comments
 (0)