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CITATION.cff

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# This CITATION.cff file was generated with cffinit.
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# Visit https://bit.ly/cffinit to generate yours today!
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cff-version: 1.2.0
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title: >-
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Workflow to Fetch Public Sequencing Data and Metadata
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Using iSeq
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message: >-
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If you use this software, please cite it using the
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metadata from this file.
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type: software
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authors:
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- given-names: Stephan
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family-names: Reichl
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orcid: 'https://orcid.org/0000-0001-8555-7198'
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affiliation: CeMM Research Center for Molecular Medicine
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- given-names: Christoph
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family-names: Bock
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orcid: 'https://orcid.org/0000-0001-6091-3088'
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affiliation: CeMM Research Center for Molecular Medicine
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repository-code: 'https://github.com/epigen/fetch_ngs'
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url: 'https://epigen.github.io/fetch_ngs/'
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abstract: >-
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A Snakemake workflow to fetch (download) and process
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public sequencing data and metadata from GSA, SRA, ENA,
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GEO and DDBJ databases using iSeq.
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keywords:
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- Bioinformatics
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- Workflow
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- Databases
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- Metadata
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- NGS
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- Snakemake
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license: MIT

README.md

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[![MrBiomics](https://img.shields.io/badge/MrBiomics-red)](https://github.com/epigen/MrBiomics/)
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[![](https://tokei.rs/b1/github/epigen/fetch_ngs?category=code)]()
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[![](https://tokei.rs/b1/github/epigen/fetch_ngs?category=files)]()
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[![GitHub license](https://img.shields.io/github/license/epigen/fetch_ngs)](https://github.com/epigen/fetch_ngs/blob/master/LICENSE)
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[![GitHub license](https://img.shields.io/github/license/epigen/fetch_ngs)](https://github.com/epigen/fetch_ngs/blob/main/LICENSE)
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![GitHub Release](https://img.shields.io/github/v/release/epigen/fetch_ngs)
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[![Snakemake](https://img.shields.io/badge/Snakemake->=8.20.1-green)](https://snakemake.readthedocs.io/en/stable/)
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# Retrieval Workflow for Public Sequencing Data Using iSeq
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A [Snakemake 8](https://snakemake.readthedocs.io/en/stable/) workflow for downloading and processing public sequencing data and metadata from **[GSA](https://ngdc.cncb.ac.cn/gsa/)**, **[SRA](https://www.ncbi.nlm.nih.gov/sra/)**, **[ENA](https://www.ebi.ac.uk/ena/)**, and **[DDBJ](https://www.ddbj.nig.ac.jp/)** databases using [iSeq](https://github.com/BioOmics/iSeq).
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# Workflow to Fetch Public Sequencing Data and Metadata Using iSeq
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A [Snakemake 8](https://snakemake.readthedocs.io/en/stable/) workflow to fetch (download) and process public sequencing data and metadata from **[GSA](https://ngdc.cncb.ac.cn/gsa/)**, **[SRA](https://www.ncbi.nlm.nih.gov/sra/)**, **[ENA](https://www.ebi.ac.uk/ena/)**, **[GEO](https://www.ncbi.nlm.nih.gov/geo/)** and **[DDBJ](https://www.ddbj.nig.ac.jp/)** databases using [iSeq](https://github.com/BioOmics/iSeq).
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> [!NOTE]
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> This workflow adheres to the module specifications of [MrBiomics](https://github.com/epigen/MrBiomics), an effort to augment research by modularizing (biomedical) data science. For more details, instructions, and modules check out the project's repository.

workflow/Snakefile

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rule all:
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input:
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os.path.join(result_path,"metadata.csv"),
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params:
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metadata_only = config["metadata_only"],
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resources:
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mem_mb=config.get("mem", "4000"),
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threads: 1

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