diff --git a/src/ethereum/osaka/fork.py b/src/ethereum/osaka/fork.py
index 22c07e0ae8..b0db4145da 100644
--- a/src/ethereum/osaka/fork.py
+++ b/src/ethereum/osaka/fork.py
@@ -852,11 +852,6 @@ def process_transaction(
     index:
         Index of the transaction in the block.
     """
-    trie_set(
-        block_output.transactions_trie,
-        rlp.encode(index),
-        encode_transaction(tx),
-    )
 
     intrinsic_gas, calldata_floor_gas_cost = validate_transaction(tx)
 
@@ -921,6 +916,7 @@ def process_transaction(
         blob_versioned_hashes=blob_versioned_hashes,
         authorizations=authorizations,
         index_in_block=index,
+        transactions_root=root(block_output.transactions_trie),
         tx_hash=get_transaction_hash(encode_transaction(tx)),
         traces=[],
     )
@@ -988,6 +984,12 @@ def process_transaction(
         receipt,
     )
 
+    trie_set(
+        block_output.transactions_trie,
+        rlp.encode(index),
+        encode_transaction(tx),
+    )
+
     block_output.block_logs += tx_output.logs
 
 
diff --git a/src/ethereum/osaka/vm/__init__.py b/src/ethereum/osaka/vm/__init__.py
index df75f66a6e..ea956b9d95 100644
--- a/src/ethereum/osaka/vm/__init__.py
+++ b/src/ethereum/osaka/vm/__init__.py
@@ -107,6 +107,7 @@ class TransactionEnvironment:
     blob_versioned_hashes: Tuple[VersionedHash, ...]
     authorizations: Tuple[Authorization, ...]
     index_in_block: Optional[Uint]
+    transactions_root: Hash32
     tx_hash: Optional[Hash32]
     traces: List[dict]
 
diff --git a/src/ethereum/osaka/vm/instructions/__init__.py b/src/ethereum/osaka/vm/instructions/__init__.py
index 9cc30668e7..322ef5cbcf 100644
--- a/src/ethereum/osaka/vm/instructions/__init__.py
+++ b/src/ethereum/osaka/vm/instructions/__init__.py
@@ -100,6 +100,7 @@ class Ops(enum.Enum):
     BASEFEE = 0x48
     BLOBHASH = 0x49
     BLOBBASEFEE = 0x4A
+    TXROOT = 0x4B
 
     # Control Flow Ops
     STOP = 0x00
@@ -252,6 +253,7 @@ class Ops(enum.Enum):
     Ops.PREVRANDAO: block_instructions.prev_randao,
     Ops.GASLIMIT: block_instructions.gas_limit,
     Ops.CHAINID: block_instructions.chain_id,
+    Ops.TXROOT: block_instructions.tx_root,
     Ops.MLOAD: memory_instructions.mload,
     Ops.MSTORE: memory_instructions.mstore,
     Ops.MSTORE8: memory_instructions.mstore8,
diff --git a/src/ethereum/osaka/vm/instructions/block.py b/src/ethereum/osaka/vm/instructions/block.py
index 80644000fd..369ed9318f 100644
--- a/src/ethereum/osaka/vm/instructions/block.py
+++ b/src/ethereum/osaka/vm/instructions/block.py
@@ -253,3 +253,32 @@ def chain_id(evm: Evm) -> None:
 
     # PROGRAM COUNTER
     evm.pc += Uint(1)
+
+
+def tx_root(evm: Evm) -> None:
+    """
+    Push the incremental transactions root of this block onto the stack.
+
+    Parameters
+    ----------
+    evm :
+        The current EVM frame.
+
+    Raises
+    ------
+    :py:class:`~ethereum.osaka.vm.exceptions.StackOverflowError`
+        If `len(stack)` is equal to `1024`.
+    :py:class:`~ethereum.osaka.vm.exceptions.OutOfGasError`
+        If `evm.gas_left` is less than `2`.
+    """
+    # STACK
+    pass
+
+    # GAS
+    charge_gas(evm, GAS_BASE)
+
+    # OPERATION
+    push(evm.stack, U256.from_be_bytes(evm.message.tx_env.transactions_root))
+
+    # PROGRAM COUNTER
+    evm.pc += Uint(1)