diff --git a/packages/react-native/Libraries/Components/AccessibilityInfo/AccessibilityInfo.d.ts b/packages/react-native/Libraries/Components/AccessibilityInfo/AccessibilityInfo.d.ts
index bad75fca1346b6..e74482135c032d 100644
--- a/packages/react-native/Libraries/Components/AccessibilityInfo/AccessibilityInfo.d.ts
+++ b/packages/react-native/Libraries/Components/AccessibilityInfo/AccessibilityInfo.d.ts
@@ -13,6 +13,7 @@ import {EmitterSubscription} from '../../vendor/emitter/EventEmitter';
 type AccessibilityChangeEventName =
   | 'change' // deprecated, maps to screenReaderChanged
   | 'boldTextChanged' // iOS-only Event
+  | 'differentiateWithoutColorChanged' // iOS-only Event
   | 'grayscaleChanged' // iOS-only Event
   | 'invertColorsChanged' // iOS-only Event
   | 'reduceMotionChanged'
@@ -51,6 +52,13 @@ export interface AccessibilityInfoStatic {
    */
   isBoldTextEnabled: () => Promise<boolean>;
 
+  /**
+   * Query whether Differentiate Without Color is currently enabled.
+   *
+   * @platform ios
+   */
+  isDifferentiateWithoutColorEnabled: () => Promise<boolean>;
+
   /**
    * Query whether grayscale is currently enabled.
    *
diff --git a/packages/react-native/Libraries/Components/AccessibilityInfo/AccessibilityInfo.js b/packages/react-native/Libraries/Components/AccessibilityInfo/AccessibilityInfo.js
index 9e1d236c92883c..2d5a5d5cad17f0 100644
--- a/packages/react-native/Libraries/Components/AccessibilityInfo/AccessibilityInfo.js
+++ b/packages/react-native/Libraries/Components/AccessibilityInfo/AccessibilityInfo.js
@@ -109,6 +109,31 @@ const AccessibilityInfo = {
     }
   },
 
+  /**
+   * Query whether Differentiate Without Color is currently enabled. iOS only.
+   *
+   * Returns a promise which resolves to a boolean.
+   * The result is `true` when the Differentiate Without Color setting is enabled and `false` otherwise.
+   *
+   * See https://reactnative.dev/docs/accessibilityinfo#isDifferentiateWithoutColorEnabled
+   */
+  isDifferentiateWithoutColorEnabled(): Promise<boolean> {
+    if (Platform.OS === 'android') {
+      return Promise.resolve(false);
+    } else {
+      return new Promise((resolve, reject) => {
+        if (NativeAccessibilityManagerIOS != null) {
+          NativeAccessibilityManagerIOS.getCurrentDifferentiateWithoutColorState(
+            resolve,
+            reject,
+          );
+        } else {
+          reject(new Error('NativeAccessibilityManagerIOS is not available'));
+        }
+      });
+    }
+  },
+
   /**
    * Query whether grayscale is currently enabled.
    *
diff --git a/packages/react-native/React/CoreModules/RCTAccessibilityManager.h b/packages/react-native/React/CoreModules/RCTAccessibilityManager.h
index e8ea831b931b0d..c70334b7cdd2a4 100644
--- a/packages/react-native/React/CoreModules/RCTAccessibilityManager.h
+++ b/packages/react-native/React/CoreModules/RCTAccessibilityManager.h
@@ -21,6 +21,7 @@ extern NSString *const RCTAccessibilityManagerDidUpdateMultiplierNotification; /
 @property (nonatomic, copy) NSDictionary<NSString *, NSNumber *> *multipliers;
 
 @property (nonatomic, assign) BOOL isBoldTextEnabled;
+@property (nonatomic, assign) BOOL isDifferentiateWithoutColorEnabled;
 @property (nonatomic, assign) BOOL isGrayscaleEnabled;
 @property (nonatomic, assign) BOOL isInvertColorsEnabled;
 @property (nonatomic, assign) BOOL isReduceMotionEnabled;
diff --git a/packages/react-native/React/CoreModules/RCTAccessibilityManager.mm b/packages/react-native/React/CoreModules/RCTAccessibilityManager.mm
index 19895271f14104..fc8ae1cd1b37f6 100644
--- a/packages/react-native/React/CoreModules/RCTAccessibilityManager.mm
+++ b/packages/react-native/React/CoreModules/RCTAccessibilityManager.mm
@@ -61,6 +61,11 @@ - (instancetype)init
                                                  name:UIAccessibilityBoldTextStatusDidChangeNotification
                                                object:nil];
 
+    [[NSNotificationCenter defaultCenter] addObserver:self
+                                             selector:@selector(differentiateWithoutColorStatusDidChange:)
+                                                 name:UIAccessibilityDifferentiateWithoutColorStatusDidChangeNotification
+                                               object:nil];
+
     [[NSNotificationCenter defaultCenter] addObserver:self
                                              selector:@selector(grayscaleStatusDidChange:)
                                                  name:UIAccessibilityGrayscaleStatusDidChangeNotification
@@ -93,6 +98,7 @@ - (instancetype)init
 
     self.contentSizeCategory = RCTSharedApplication().preferredContentSizeCategory;
     _isBoldTextEnabled = UIAccessibilityIsBoldTextEnabled();
+    _isDifferentiateWithoutColorEnabled = UIAccessibilityIsDifferentiateWithoutColorEnabled();
     _isGrayscaleEnabled = UIAccessibilityIsGrayscaleEnabled();
     _isInvertColorsEnabled = UIAccessibilityIsInvertColorsEnabled();
     _isReduceMotionEnabled = UIAccessibilityIsReduceMotionEnabled();
@@ -136,6 +142,19 @@ - (void)boldTextStatusDidChange:(__unused NSNotification *)notification
   }
 }
 
+- (void)differentiateWithoutColorStatusDidChange:(__unused NSNotification *)notification
+{
+  BOOL newDifferentiateWithoutColorEnabled = UIAccessibilityIsDifferentiateWithoutColorEnabled();
+  if (_isDifferentiateWithoutColorEnabled != newDifferentiateWithoutColorEnabled) {
+    _isDifferentiateWithoutColorEnabled = newDifferentiateWithoutColorEnabled;
+#pragma clang diagnostic push
+#pragma clang diagnostic ignored "-Wdeprecated-declarations"
+    [[_moduleRegistry moduleForName:"EventDispatcher"] sendDeviceEventWithName:@"differentiateWithoutColorChanged"
+                                                                          body:@(_isDifferentiateWithoutColorEnabled)];
+#pragma clang diagnostic pop
+  }
+}
+
 - (void)grayscaleStatusDidChange:(__unused NSNotification *)notification
 {
   BOOL newGrayscaleEnabled = UIAccessibilityIsGrayscaleEnabled();
@@ -369,6 +388,12 @@ static void setMultipliers(
   onSuccess(@[ @(_isBoldTextEnabled) ]);
 }
 
+RCT_EXPORT_METHOD(
+    getCurrentDifferentiateWithoutColorState : (RCTResponseSenderBlock)onSuccess onError : (__unused RCTResponseSenderBlock)onError)
+{
+  onSuccess(@[ @(_isDifferentiateWithoutColorEnabled) ]);
+}
+
 RCT_EXPORT_METHOD(
     getCurrentGrayscaleState : (RCTResponseSenderBlock)onSuccess onError : (__unused RCTResponseSenderBlock)onError)
 {
diff --git a/packages/react-native/jest/mocks/AccessibilityInfo.js b/packages/react-native/jest/mocks/AccessibilityInfo.js
index 4dc215bcabd7f4..297e7d02f41c35 100644
--- a/packages/react-native/jest/mocks/AccessibilityInfo.js
+++ b/packages/react-native/jest/mocks/AccessibilityInfo.js
@@ -24,6 +24,10 @@ const AccessibilityInfo = {
     $FlowFixMe,
     $FlowFixMe,
   >,
+  isDifferentiateWithoutColorEnabled: jest.fn(() => Promise.resolve(false)) as JestMockFn<
+    $FlowFixMe,
+    $FlowFixMe,
+  >,
   isGrayscaleEnabled: jest.fn(() => Promise.resolve(false)) as JestMockFn<
     $FlowFixMe,
     $FlowFixMe,
diff --git a/packages/react-native/src/private/specs_DEPRECATED/modules/NativeAccessibilityManager.js b/packages/react-native/src/private/specs_DEPRECATED/modules/NativeAccessibilityManager.js
index de79663d418b39..cef9b4df6b1e2f 100644
--- a/packages/react-native/src/private/specs_DEPRECATED/modules/NativeAccessibilityManager.js
+++ b/packages/react-native/src/private/specs_DEPRECATED/modules/NativeAccessibilityManager.js
@@ -17,6 +17,10 @@ export interface Spec extends TurboModule {
     onSuccess: (isBoldTextEnabled: boolean) => void,
     onError: (error: Object) => void,
   ) => void;
+  +getCurrentDifferentiateWithoutColorState?: (
+    onSuccess: (isDifferentiateWithoutColorEnabled: boolean) => void,
+    onError: (error: Object) => void,
+  ) => void;
   +getCurrentGrayscaleState: (
     onSuccess: (isGrayscaleEnabled: boolean) => void,
     onError: (error: Object) => void,
diff --git a/packages/react-native/types/__typetests__/index.tsx b/packages/react-native/types/__typetests__/index.tsx
index 47f720b21946a7..2a24cb8c939d43 100644
--- a/packages/react-native/types/__typetests__/index.tsx
+++ b/packages/react-native/types/__typetests__/index.tsx
@@ -1473,6 +1473,9 @@ class AccessibilityTest extends React.Component {
 AccessibilityInfo.isBoldTextEnabled().then(isEnabled =>
   console.log(`AccessibilityInfo.isBoldTextEnabled => ${isEnabled}`),
 );
+AccessibilityInfo.isDifferentiateWithoutColorEnabled().then(isEnabled =>
+  console.log(`AccessibilityInfo.isDifferentiateWithoutColorEnabled => ${isEnabled}`),
+);
 AccessibilityInfo.isGrayscaleEnabled().then(isEnabled =>
   console.log(`AccessibilityInfo.isGrayscaleEnabled => ${isEnabled}`),
 );
@@ -1504,6 +1507,9 @@ AccessibilityInfo.addEventListener(
 AccessibilityInfo.addEventListener('boldTextChanged', isEnabled =>
   console.log(`AccessibilityInfo.isBoldTextEnabled => ${isEnabled}`),
 );
+AccessibilityInfo.addEventListener('differentiateWithoutColorChanged', isEnabled =>
+  console.log(`AccessibilityInfo.isDifferentiateWithoutColorEnabled => ${isEnabled}`),
+);
 AccessibilityInfo.addEventListener('grayscaleChanged', isEnabled =>
   console.log(`AccessibilityInfo.isGrayscaleEnabled => ${isEnabled}`),
 );