Volume Electron Microscopy (VEM) enables the capture of 3D structure beyond planar samples, which is crucial for understanding biological mechanisms. With automation, improved resolution, and increased data storage capacity, VEM has led to an explosion of large three-dimensional datasets. Large datasets offer the opportunity to generate statistical data, but analysing them often requires assigning each voxel (3D pixel) to its corresponding structure, a process known as image segmentation. Manually segmenting hundreds or thousands of image slices is tedious and time-consuming. Computer-aided, especially Machine Learning (ML) based segmentation is now a routinely used method, with Trainable Weka Segmentation [@arganda2017trainable] and Ilastik [@berg2019ilastik] being two leading options. Emerging methods for EM image segmentation are often based on Deep Learning (DL) [@mekuvc2020automatic] because this approach has potential to outperform traditional ML in terms of accuracy and adaptivity [@minaee2021image][@erickson2019deep].
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