Merge pull request #658 from RZ9082/mgnify_amplicon_pipeline

Mgnify amplicon pipeline

Author: bgruening

workflows/amplicon-mgnify/README.md

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+# MGnify Amplicon Workflow and Add-on-workflows
+
+This directory contains the following workflows:
+* **MGnify amplicon workflow v5.0** and its sub-workflows:
+    * **Quality control for single-end reads**
+    * **Quality control for paired-end reads**
+    * **rRNA-prediction**
+    * **ITS**
+    * **Summary tables**

workflows/amplicon-mgnify/mapseq-to-ampvis2/.dockstore.yml

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+version: 1.2
+workflows:
+- name: main
+  subclass: Galaxy
+  publish: true
+  primaryDescriptorPath: /mapseq-to-ampvis2.ga
+  testParameterFiles:
+  - /mapseq-to-ampvis2-tests.yml
+  authors:
+  - name: Rand Zoabi
+    orcid: 0009-0000-2501-8053
+  - name: Mara Besemer
+    url: https://github.com/MaraBesemer

workflows/amplicon-mgnify/mapseq-to-ampvis2/CHANGELOG.md

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+# Changelog
+
+## [0.1] - 07-06-2024
+
+- First release

workflows/amplicon-mgnify/mapseq-to-ampvis2/README.md

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+# MAPseq to ampvis workflow
+
+The MAPseq to Ampvis workflow processes MAPseq OTU tables and associated metadata for analysis in Ampvis2. This workflow involves reformatting MAPseq output datasets to produce structured output files suitable for Ampvis2.
+
+## Input datasets
+
+- **MAPseq OTU tables** These tables, generated by MAPseq, include sequence counts and taxonomic classifications.
+- **Metadata** Contextual information associated with the data from the OTU tables.
+
+## Outputs
+
+- **otu_table** A newly formatted OTU table structured for Ampvis2.
+- **tax_table** A taxonomy table containing the taxonomic classifications from the OTU tables.
+- **ampvis2** An R object compatible with the Ampvis2 tool for further analysis.
+- **metadata_list_out**  A structured list of metadata used in the analysis.
+- **taxonomy_list_out** A processed taxonomy list ready for integration with Ampvis2.

workflows/amplicon-mgnify/mapseq-to-ampvis2/mapseq-to-ampvis2-tests.yml

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+- doc: Test outline for mapseq-to-ampvis2.ga
+  job:
+    MAPseq OTU tables:
+      class: Collection
+      collection_type: list
+      elements:
+      - class: File
+        identifier: SRR11032273
+        location: https://zenodo.org/records/13347829/files/SRR11032273.tsv
+      - class: File
+        identifier: SRR11032274
+        location: https://zenodo.org/records/13347829/files/SRR11032274.tsv
+      - class: File
+        identifier: SRR11032275
+        location: https://zenodo.org/records/13347829/files/SRR11032275.tsv
+      - class: File
+        identifier: SRR11032276
+        location: https://zenodo.org/records/13347829/files/SRR11032276.tsv
+      - class: File
+        identifier: SRR11032277
+        location: https://zenodo.org/records/13347829/files/SRR11032277.tsv
+      - class: File
+        identifier: SRR11038211
+        location: https://zenodo.org/records/13347829/files/SRR11038211.tsv
+      - class: File
+        identifier: SRR11038212
+        location: https://zenodo.org/records/13347829/files/SRR11038212.tsv
+      - class: File
+        identifier: SRR11038213
+        location: https://zenodo.org/records/13347829/files/SRR11038213.tsv
+      - class: File
+        identifier: SRR11038214
+        location: https://zenodo.org/records/13347829/files/SRR11038214.tsv
+      - class: File
+        identifier: SRR11038215
+        location: https://zenodo.org/records/13347829/files/SRR11038215.tsv
+    OTU table metadata:
+      class: File
+      location: https://zenodo.org/records/13347829/files/test_metadata_formatted.tabular
+  outputs:
+    OTU table:
+      location: https://zenodo.org/records/13347829/files/OTU_table.tabular
+    Taxonomy table:
+      location: https://zenodo.org/records/14745803/files/tax_table.tabular
+    Ampvis2 object:
+      asserts:
+        - that: has_size
+          value: 15000
+          delta: 1000
+    Ampvis2 metadata table:
+      location: https://zenodo.org/records/13347829/files/metadata_list.tabular
+    Ampvis2 taxonomy table:
+      location: https://zenodo.org/records/14745803/files/taxonomy_list.tabular