@@ -5,10 +5,10 @@ title: "Assembly of metagenomic sequencing data"
55zenodo_link : " https://zenodo.org/record/7818827"
66level : Introductory
77questions :
8- - " Why metagenomic data should be assembled?"
8+ - " Why should metagenomic data be assembled?"
99 - " What is the difference between co-assembly and individual assembly?"
1010 - " What is the difference between reads, contigs and scaffolds?"
11- - " How tools based on De Bruijn graph work?"
11+ - " How tools based on de Bruijn graph work?"
1212 - " How to assess the quality of metagenomic data assembly?"
1313objectives :
1414 - " Describe what an assembly is"
@@ -23,7 +23,7 @@ objectives:
2323time_estimation : " 2H"
2424key_points :
2525 - " Assembly groups reads into contigs and scafolds."
26- - " De Brujin Graphs use k-mers to assembly reads"
26+ - " de Brujin Graphs use k-mers to assembly reads"
2727 - " MetaSPAdes and MEGAHIT are assemblers"
2828 - " Quast is the tool to assess the assembly quality"
2929edam_ontology :
@@ -33,6 +33,7 @@ contributions:
3333 authorship :
3434 - plushz
3535 - bebatut
36+ - vinisalazar
3637 funding :
3738 - gallantries
3839subtopic : metagenomics
@@ -124,30 +125,39 @@ In case of a not very large dataset it's more convenient to upload data directly
124125
125126> <hands-on-title >Upload data into Galaxy</hands-on-title >
126127>
127- > 2 . Import the sequence read raw data ( \* .fastqsanger.gz) from [ Zenodo ] ({{ page.zenodo_link }}) or a data library:
128+ > 2 . The information we need to import the samples for this tutorial (Sample ID, and link to the FASTQ file (URL) are in the grey box below.
128129>
129130> ``` text
130- > {{ page.zenodo_link }}/files/ERR2231567_1.fastqsanger.gz
131- > {{ page.zenodo_link }}/files/ERR2231567_2.fastqsanger.gz
132- > {{ page.zenodo_link }}/files/ERR2231568_1.fastqsanger.gz
133- > {{ page.zenodo_link }}/files/ERR2231568_2.fastqsanger.gz
134- > {{ page.zenodo_link }}/files/ERR2231569_1.fastqsanger.gz
135- > {{ page.zenodo_link }}/files/ERR2231569_2.fastqsanger.gz
136- > {{ page.zenodo_link }}/files/ERR2231570_1.fastqsanger.gz
137- > {{ page.zenodo_link }}/files/ERR2231570_2.fastqsanger.gz
138- > {{ page.zenodo_link }}/files/ERR2231571_1.fastqsanger.gz
139- > {{ page.zenodo_link }}/files/ERR2231571_2.fastqsanger.gz
140- > {{ page.zenodo_link }}/files/ERR2231572_1.fastqsanger.gz
141- > {{ page.zenodo_link }}/files/ERR2231572_2.fastqsanger.gz
131+ > SampleID URL
132+ > ERR2231567_1 {{ page.zenodo_link }}/files/ERR2231567_1.fastqsanger.gz
133+ > ERR2231567_2 {{ page.zenodo_link }}/files/ERR2231567_2.fastqsanger.gz
134+ > ERR2231568_1 {{ page.zenodo_link }}/files/ERR2231568_1.fastqsanger.gz
135+ > ERR2231568_2 {{ page.zenodo_link }}/files/ERR2231568_2.fastqsanger.gz
136+ > ERR2231569_1 {{ page.zenodo_link }}/files/ERR2231569_1.fastqsanger.gz
137+ > ERR2231569_2 {{ page.zenodo_link }}/files/ERR2231569_2.fastqsanger.gz
138+ > ERR2231570_1 {{ page.zenodo_link }}/files/ERR2231570_1.fastqsanger.gz
139+ > ERR2231570_2 {{ page.zenodo_link }}/files/ERR2231570_2.fastqsanger.gz
140+ > ERR2231571_1 {{ page.zenodo_link }}/files/ERR2231571_1.fastqsanger.gz
141+ > ERR2231571_2 {{ page.zenodo_link }}/files/ERR2231571_2.fastqsanger.gz
142+ > ERR2231572_1 {{ page.zenodo_link }}/files/ERR2231572_1.fastqsanger.gz
143+ > ERR2231572_2 {{ page.zenodo_link }}/files/ERR2231572_2.fastqsanger.gz
142144> ```
143145>
144146> {% snippet faqs/galaxy/datasets_import_via_link.md %}
145147>
146- > {% snippet faqs/galaxy/datasets_import_from_data_library.md %}
148+ > Import the files from Zenodo using Galaxy's Rule-based Uploader.
149+ > - Open the Galaxy Upload Manager
150+ > - Click the tab **Rule-based**
151+ > - Paste the table.
152+ > - Remove the first line.
153+ > - Click **Build**
154+ > 
155+ > - On the "Rules" pane on the left, click on "Click here" to set column definitions.
156+ > 
157+ > - Set the first column as "Name" and the second column as "URL".
158+ > 
159+ > - Click "Apply" and then "Upload".
147160>
148- > > <comment-title></comment-title>
149- > > In case of large dataset, we can use FTP server or the [Galaxy Rule-based Uploader]({% link topics/galaxy-interface/tutorials/upload-rules/tutorial.md %}).
150- > {: .comment}
151161>
152162> 3. Create a paired collection named `Raw reads`, rename your pairs with the sample name
153163>
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