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97 | 97 | ??? |
98 | | -- With single cell RNA-seq analysis, the stage shifts away from measuring the average expression of a tissue, and towards measuring the specific gene expression of individual cells within those tissues. |
| 98 | +- With single cell RNA-seq analysis, the stage shifts away from measuring the average expression of a tissue. |
| 99 | +- And towards measuring the specific gene expression of individual cells within those tissues. |
99 | 100 | - Here we are no longer comparing tissue against tissue, but cell against cell. |
100 | 101 | - Each cell is assigned a gene profile which describes the relative abundance of genes detected within it. |
101 | 102 | - Many cells share the same gene profile, where a gene profile ideally describes a cell type. |
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168 | 169 | ??? |
169 | | -- For single cell analysis, the process is much the same, except that each sample is a cell, and must therefore be sequenced separately from other cells. |
| 170 | +- For single cell analysis, the process is much the same, except that each sample is a cell. |
| 171 | +- And must therefore be sequenced separately from other cells. |
170 | 172 | - Once isolated, unique barcodes are added to each cell, and then sequenced. |
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187 | 189 | ??? |
188 | | -Because the level of resolution in single-cell is at the cell level, and that each cell is unique, the concept biological replicates is not quite the same as that in bulk RNA-seq. |
| 190 | +- The level of resolution in single-cell is at the cell level, and each cell is unique. |
| 191 | +- Therefore, the concept biological replicates is not quite the same as that in bulk RNA-seq. |
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191 | 194 | --- |
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321 | 324 | ??? |
322 | | -Forward scatter is aligned with the main laser and measures the diameter of cell, which is ideal for distinguishing different cells by their size profiles, such as monocytes which are typically larger than lymphocytes as seen on the X-axis of the example image. |
| 325 | +- Forward scatter is aligned with the main laser and measures the diameter of cell, which is ideal for distinguishing different cells by their size profiles. |
| 326 | +- For example monocytes, which are typically larger than lymphocytes, as seen on the X-axis of the example image. |
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