diff --git a/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-custom-sampler/.gitignore b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-custom-sampler/.gitignore
new file mode 100644
index 000000000000..686a0277246c
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-custom-sampler/.gitignore
@@ -0,0 +1,2 @@
+dist
+.vscode
diff --git a/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-custom-sampler/.npmrc b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-custom-sampler/.npmrc
new file mode 100644
index 000000000000..070f80f05092
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-custom-sampler/.npmrc
@@ -0,0 +1,2 @@
+@sentry:registry=http://127.0.0.1:4873
+@sentry-internal:registry=http://127.0.0.1:4873
diff --git a/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-custom-sampler/package.json b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-custom-sampler/package.json
new file mode 100644
index 000000000000..d445419ece51
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-custom-sampler/package.json
@@ -0,0 +1,36 @@
+{
+  "name": "node-core-express-otel-v1-custom-sampler",
+  "version": "1.0.0",
+  "private": true,
+  "scripts": {
+    "build": "tsc",
+    "start": "node dist/app.js",
+    "test": "playwright test",
+    "clean": "npx rimraf node_modules pnpm-lock.yaml",
+    "test:build": "pnpm install && pnpm build",
+    "test:assert": "pnpm test"
+  },
+  "dependencies": {
+    "@opentelemetry/api": "^1.9.0",
+    "@opentelemetry/context-async-hooks": "^1.30.1",
+    "@opentelemetry/core": "^1.30.1",
+    "@opentelemetry/instrumentation": "^0.57.1",
+    "@opentelemetry/instrumentation-http": "^0.57.1",
+    "@opentelemetry/resources": "^1.30.1",
+    "@opentelemetry/sdk-trace-node": "^1.30.1",
+    "@opentelemetry/semantic-conventions": "^1.30.0",
+    "@sentry/node-core": "latest || *",
+    "@sentry/opentelemetry": "latest || *",
+    "@types/express": "4.17.17",
+    "@types/node": "^18.19.1",
+    "express": "4.19.2",
+    "typescript": "~5.0.0"
+  },
+  "devDependencies": {
+    "@playwright/test": "~1.53.2",
+    "@sentry-internal/test-utils": "link:../../../test-utils"
+  },
+  "volta": {
+    "extends": "../../package.json"
+  }
+}
diff --git a/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-custom-sampler/playwright.config.mjs b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-custom-sampler/playwright.config.mjs
new file mode 100644
index 000000000000..31f2b913b58b
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-custom-sampler/playwright.config.mjs
@@ -0,0 +1,7 @@
+import { getPlaywrightConfig } from '@sentry-internal/test-utils';
+
+const config = getPlaywrightConfig({
+  startCommand: `pnpm start`,
+});
+
+export default config;
diff --git a/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-custom-sampler/src/app.ts b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-custom-sampler/src/app.ts
new file mode 100644
index 000000000000..e5da185262ad
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-custom-sampler/src/app.ts
@@ -0,0 +1,51 @@
+import './instrument';
+
+import * as Sentry from '@sentry/node-core';
+import express from 'express';
+
+const PORT = 3030;
+const app = express();
+
+const wait = (duration: number) => {
+  return new Promise<void>(res => {
+    setTimeout(() => res(), duration);
+  });
+};
+
+app.get('/task', async (_req, res) => {
+  await Sentry.startSpan({ name: 'Long task', op: 'custom.op' }, async () => {
+    await wait(200);
+  });
+  res.send('ok');
+});
+
+app.get('/unsampled/task', async (_req, res) => {
+  await wait(200);
+  res.send('ok');
+});
+
+app.get('/test-error', async function (req, res) {
+  const exceptionId = Sentry.captureException(new Error('This is an error'));
+
+  await Sentry.flush(2000);
+
+  res.send({ exceptionId });
+});
+
+app.get('/test-exception/:id', function (req, _res) {
+  throw new Error(`This is an exception with id ${req.params.id}`);
+});
+
+app.use(function onError(err: unknown, req: any, res: any, next: any) {
+  // Explicitly capture the error with Sentry
+  Sentry.captureException(err);
+
+  // The error id is attached to `res.sentry` to be returned
+  // and optionally displayed to the user for support.
+  res.statusCode = 500;
+  res.end(res.sentry + '\n');
+});
+
+app.listen(PORT, () => {
+  console.log('App listening on ', PORT);
+});
diff --git a/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-custom-sampler/src/custom-sampler.ts b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-custom-sampler/src/custom-sampler.ts
new file mode 100644
index 000000000000..cbaaac57c8ea
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-custom-sampler/src/custom-sampler.ts
@@ -0,0 +1,31 @@
+import { Attributes, Context, Link, SpanKind } from '@opentelemetry/api';
+import { Sampler, SamplingResult } from '@opentelemetry/sdk-trace-node';
+import { wrapSamplingDecision } from '@sentry/opentelemetry';
+
+export class CustomSampler implements Sampler {
+  public shouldSample(
+    context: Context,
+    _traceId: string,
+    _spanName: string,
+    _spanKind: SpanKind,
+    attributes: Attributes,
+    _links: Link[],
+  ): SamplingResult {
+    const route = attributes['http.route'];
+    const target = attributes['http.target'];
+    const decision =
+      (typeof route === 'string' && route.includes('/unsampled')) ||
+      (typeof target === 'string' && target.includes('/unsampled'))
+        ? 0
+        : 1;
+    return wrapSamplingDecision({
+      decision,
+      context,
+      spanAttributes: attributes,
+    });
+  }
+
+  public toString(): string {
+    return CustomSampler.name;
+  }
+}
diff --git a/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-custom-sampler/src/instrument.ts b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-custom-sampler/src/instrument.ts
new file mode 100644
index 000000000000..b01601ad8910
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-custom-sampler/src/instrument.ts
@@ -0,0 +1,27 @@
+import { NodeTracerProvider } from '@opentelemetry/sdk-trace-node';
+import { HttpInstrumentation } from '@opentelemetry/instrumentation-http';
+import * as Sentry from '@sentry/node-core';
+import { SentryPropagator, SentrySpanProcessor } from '@sentry/opentelemetry';
+import { CustomSampler } from './custom-sampler';
+
+Sentry.init({
+  environment: 'qa', // dynamic sampling bias to keep transactions
+  dsn: process.env.E2E_TEST_DSN,
+  includeLocalVariables: true,
+  debug: !!process.env.DEBUG,
+  tunnel: `http://localhost:3031/`, // proxy server
+  tracesSampleRate: 1,
+  openTelemetryInstrumentations: [new HttpInstrumentation()],
+});
+
+const provider = new NodeTracerProvider({
+  sampler: new CustomSampler(),
+  spanProcessors: [new SentrySpanProcessor()],
+});
+
+provider.register({
+  propagator: new SentryPropagator(),
+  contextManager: new Sentry.SentryContextManager(),
+});
+
+Sentry.validateOpenTelemetrySetup();
diff --git a/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-custom-sampler/start-event-proxy.mjs b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-custom-sampler/start-event-proxy.mjs
new file mode 100644
index 000000000000..fd7ba2bfcbc2
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-custom-sampler/start-event-proxy.mjs
@@ -0,0 +1,6 @@
+import { startEventProxyServer } from '@sentry-internal/test-utils';
+
+startEventProxyServer({
+  port: 3031,
+  proxyServerName: 'node-core-express-otel-v1-custom-sampler',
+});
diff --git a/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-custom-sampler/tests/errors.test.ts b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-custom-sampler/tests/errors.test.ts
new file mode 100644
index 000000000000..a5f45ddc4b52
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-custom-sampler/tests/errors.test.ts
@@ -0,0 +1,30 @@
+import { expect, test } from '@playwright/test';
+import { waitForError } from '@sentry-internal/test-utils';
+
+test('Sends correct error event', async ({ baseURL }) => {
+  const errorEventPromise = waitForError('node-core-express-otel-v1-custom-sampler', event => {
+    return !event.type && event.exception?.values?.[0]?.value === 'This is an exception with id 123';
+  });
+
+  await fetch(`${baseURL}/test-exception/123`);
+
+  const errorEvent = await errorEventPromise;
+
+  expect(errorEvent.exception?.values).toHaveLength(1);
+  expect(errorEvent.exception?.values?.[0]?.value).toBe('This is an exception with id 123');
+
+  expect(errorEvent.request).toEqual({
+    method: 'GET',
+    cookies: {},
+    headers: expect.any(Object),
+    url: 'http://localhost:3030/test-exception/123',
+  });
+
+  // For node-core without Express integration, transaction name is the actual URL
+  expect(errorEvent.transaction).toEqual('GET /test-exception/123');
+
+  expect(errorEvent.contexts?.trace).toEqual({
+    trace_id: expect.stringMatching(/[a-f0-9]{32}/),
+    span_id: expect.stringMatching(/[a-f0-9]{16}/),
+  });
+});
diff --git a/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-custom-sampler/tests/sampling.test.ts b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-custom-sampler/tests/sampling.test.ts
new file mode 100644
index 000000000000..60e2424552cd
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-custom-sampler/tests/sampling.test.ts
@@ -0,0 +1,95 @@
+import { expect, test } from '@playwright/test';
+import { waitForTransaction } from '@sentry-internal/test-utils';
+
+test('Sends a sampled API route transaction', async ({ baseURL }) => {
+  const transactionEventPromise = waitForTransaction('node-core-express-otel-v1-custom-sampler', transactionEvent => {
+    return transactionEvent?.contexts?.trace?.op === 'http.server' && transactionEvent?.transaction === 'GET /task';
+  });
+
+  await fetch(`${baseURL}/task`);
+
+  const transactionEvent = await transactionEventPromise;
+
+  expect(transactionEvent.contexts?.trace).toEqual({
+    span_id: expect.stringMatching(/[a-f0-9]{16}/),
+    trace_id: expect.stringMatching(/[a-f0-9]{32}/),
+    data: {
+      'sentry.source': 'url',
+      'sentry.op': 'http.server',
+      'sentry.origin': 'manual',
+      url: 'http://localhost:3030/task',
+      'otel.kind': 'SERVER',
+      'http.response.status_code': 200,
+      'http.url': 'http://localhost:3030/task',
+      'http.host': 'localhost:3030',
+      'net.host.name': 'localhost',
+      'http.method': 'GET',
+      'http.scheme': 'http',
+      'http.target': '/task',
+      'http.user_agent': 'node',
+      'http.flavor': '1.1',
+      'net.transport': 'ip_tcp',
+      'net.host.ip': expect.any(String),
+      'net.host.port': 3030,
+      'net.peer.ip': expect.any(String),
+      'net.peer.port': expect.any(Number),
+      'http.status_code': 200,
+      'http.status_text': 'OK',
+    },
+    origin: 'manual',
+    op: 'http.server',
+    status: 'ok',
+  });
+
+  expect(transactionEvent.spans?.length).toBe(1);
+
+  expect(transactionEvent.spans).toContainEqual({
+    span_id: expect.stringMatching(/[a-f0-9]{16}/),
+    trace_id: expect.stringMatching(/[a-f0-9]{32}/),
+    data: {
+      'sentry.origin': 'manual',
+      'sentry.op': 'custom.op',
+    },
+    description: 'Long task',
+    parent_span_id: expect.stringMatching(/[a-f0-9]{16}/),
+    start_timestamp: expect.any(Number),
+    timestamp: expect.any(Number),
+    status: 'ok',
+    op: 'custom.op',
+    origin: 'manual',
+  });
+});
+
+test('Does not send an unsampled API route transaction', async ({ baseURL }) => {
+  const unsampledTransactionEventPromise = waitForTransaction(
+    'node-core-express-otel-v1-custom-sampler',
+    transactionEvent => {
+      return (
+        transactionEvent?.contexts?.trace?.op === 'http.server' &&
+        transactionEvent?.transaction === 'GET /unsampled/task'
+      );
+    },
+  );
+
+  await fetch(`${baseURL}/unsampled/task`);
+
+  const promiseShouldNotResolve = () =>
+    new Promise<void>((resolve, reject) => {
+      const timeout = setTimeout(() => {
+        resolve(); // Test passes because promise did not resolve within timeout
+      }, 1000);
+
+      unsampledTransactionEventPromise.then(
+        () => {
+          clearTimeout(timeout);
+          reject(new Error('Promise should not have resolved'));
+        },
+        () => {
+          clearTimeout(timeout);
+          reject(new Error('Promise should not have been rejected'));
+        },
+      );
+    });
+
+  expect(promiseShouldNotResolve()).resolves.not.toThrow();
+});
diff --git a/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-custom-sampler/tsconfig.json b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-custom-sampler/tsconfig.json
new file mode 100644
index 000000000000..2887ec11a81d
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-custom-sampler/tsconfig.json
@@ -0,0 +1,11 @@
+{
+  "compilerOptions": {
+    "types": ["node"],
+    "esModuleInterop": true,
+    "lib": ["es2018"],
+    "strict": true,
+    "outDir": "dist",
+    "skipLibCheck": true
+  },
+  "include": ["src/**/*.ts"]
+}
diff --git a/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-sdk-node/.gitignore b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-sdk-node/.gitignore
new file mode 100644
index 000000000000..1521c8b7652b
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-sdk-node/.gitignore
@@ -0,0 +1 @@
+dist
diff --git a/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-sdk-node/.npmrc b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-sdk-node/.npmrc
new file mode 100644
index 000000000000..070f80f05092
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-sdk-node/.npmrc
@@ -0,0 +1,2 @@
+@sentry:registry=http://127.0.0.1:4873
+@sentry-internal:registry=http://127.0.0.1:4873
diff --git a/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-sdk-node/package.json b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-sdk-node/package.json
new file mode 100644
index 000000000000..868334df93fa
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-sdk-node/package.json
@@ -0,0 +1,38 @@
+{
+  "name": "node-core-express-otel-v1-sdk-node",
+  "version": "1.0.0",
+  "private": true,
+  "scripts": {
+    "build": "tsc",
+    "start": "node dist/app.js",
+    "test": "playwright test",
+    "clean": "npx rimraf node_modules pnpm-lock.yaml",
+    "test:build": "pnpm install && pnpm build",
+    "test:assert": "pnpm test"
+  },
+  "dependencies": {
+    "@opentelemetry/api": "^1.9.0",
+    "@opentelemetry/context-async-hooks": "^1.30.1",
+    "@opentelemetry/core": "^1.30.1",
+    "@opentelemetry/instrumentation": "^0.57.2",
+    "@opentelemetry/instrumentation-http": "^0.57.2",
+    "@opentelemetry/resources": "^1.30.1",
+    "@opentelemetry/sdk-trace-node": "^1.30.1",
+    "@opentelemetry/semantic-conventions": "^1.30.0",
+    "@opentelemetry/sdk-node": "^0.57.2",
+    "@opentelemetry/exporter-trace-otlp-http": "^0.57.2",
+    "@sentry/node-core": "latest || *",
+    "@sentry/opentelemetry": "latest || *",
+    "@types/express": "4.17.17",
+    "@types/node": "^18.19.1",
+    "express": "4.19.2",
+    "typescript": "~5.0.0"
+  },
+  "devDependencies": {
+    "@playwright/test": "~1.53.2",
+    "@sentry-internal/test-utils": "link:../../../test-utils"
+  },
+  "volta": {
+    "extends": "../../package.json"
+  }
+}
diff --git a/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-sdk-node/playwright.config.mjs b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-sdk-node/playwright.config.mjs
new file mode 100644
index 000000000000..888e61cfb2dc
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-sdk-node/playwright.config.mjs
@@ -0,0 +1,34 @@
+import { getPlaywrightConfig } from '@sentry-internal/test-utils';
+
+const config = getPlaywrightConfig(
+  {
+    startCommand: `pnpm start`,
+  },
+  {
+    webServer: [
+      {
+        command: `node ./start-event-proxy.mjs`,
+        port: 3031,
+        stdout: 'pipe',
+        stderr: 'pipe',
+      },
+      {
+        command: `node ./start-otel-proxy.mjs`,
+        port: 3032,
+        stdout: 'pipe',
+        stderr: 'pipe',
+      },
+      {
+        command: 'pnpm start',
+        port: 3030,
+        stdout: 'pipe',
+        stderr: 'pipe',
+        env: {
+          PORT: 3030,
+        },
+      },
+    ],
+  },
+);
+
+export default config;
diff --git a/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-sdk-node/src/app.ts b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-sdk-node/src/app.ts
new file mode 100644
index 000000000000..69f55b25e6ce
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-sdk-node/src/app.ts
@@ -0,0 +1,55 @@
+import './instrument';
+
+// Other imports below
+import * as Sentry from '@sentry/node-core';
+import express from 'express';
+
+const app = express();
+const port = 3030;
+
+app.get('/test-success', function (req, res) {
+  res.send({ version: 'v1' });
+});
+
+app.get('/test-param/:param', function (req, res) {
+  res.send({ paramWas: req.params.param });
+});
+
+app.get('/test-transaction', function (req, res) {
+  Sentry.withActiveSpan(null, async () => {
+    Sentry.startSpan({ name: 'test-transaction', op: 'e2e-test' }, () => {
+      Sentry.startSpan({ name: 'test-span' }, () => undefined);
+    });
+
+    await Sentry.flush();
+
+    res.send({});
+  });
+});
+
+app.get('/test-error', async function (req, res) {
+  const exceptionId = Sentry.captureException(new Error('This is an error'));
+
+  await Sentry.flush(2000);
+
+  res.send({ exceptionId });
+});
+
+app.get('/test-exception/:id', function (req, _res) {
+  throw new Error(`This is an exception with id ${req.params.id}`);
+});
+
+app.use(function onError(err: unknown, req: any, res: any, next: any) {
+  // Explicitly capture the error with Sentry because @sentry/node-core doesn't have
+  // a way to capture errors from express like @sentry/node does.
+  res.sentry = Sentry.captureException(err);
+
+  // The error id is attached to `res.sentry` to be returned
+  // and optionally displayed to the user for support.
+  res.statusCode = 500;
+  res.end(res.sentry + '\n');
+});
+
+app.listen(port, () => {
+  console.log(`Example app listening on port ${port}`);
+});
diff --git a/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-sdk-node/src/instrument.ts b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-sdk-node/src/instrument.ts
new file mode 100644
index 000000000000..276b4f55ac73
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-sdk-node/src/instrument.ts
@@ -0,0 +1,35 @@
+const opentelemetry = require('@opentelemetry/sdk-node');
+const { OTLPTraceExporter } = require('@opentelemetry/exporter-trace-otlp-http');
+const Sentry = require('@sentry/node-core');
+const { HttpInstrumentation } = require('@opentelemetry/instrumentation-http');
+const { SentrySpanProcessor, SentryPropagator, SentrySampler } = require('@sentry/opentelemetry');
+
+const sentryClient = Sentry.init({
+  environment: 'qa', // dynamic sampling bias to keep transactions
+  dsn: process.env.E2E_TEST_DSN,
+  includeLocalVariables: true,
+  debug: true,
+  tunnel: `http://localhost:3031/`, // proxy server
+  tracesSampleRate: 1,
+});
+
+if (sentryClient) {
+  const sdk = new opentelemetry.NodeSDK({
+    sampler: new SentrySampler(sentryClient),
+    textMapPropagator: new SentryPropagator(),
+    contextManager: new Sentry.SentryContextManager(),
+    spanProcessors: [
+      new SentrySpanProcessor(),
+      new opentelemetry.node.BatchSpanProcessor(
+        new OTLPTraceExporter({
+          url: 'http://localhost:3032/',
+        }),
+      ),
+    ],
+    instrumentations: [new HttpInstrumentation()],
+  });
+
+  sdk.start();
+
+  Sentry.validateOpenTelemetrySetup();
+}
diff --git a/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-sdk-node/start-event-proxy.mjs b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-sdk-node/start-event-proxy.mjs
new file mode 100644
index 000000000000..815dabeb77f5
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-sdk-node/start-event-proxy.mjs
@@ -0,0 +1,6 @@
+import { startEventProxyServer } from '@sentry-internal/test-utils';
+
+startEventProxyServer({
+  port: 3031,
+  proxyServerName: 'node-core-express-otel-v1-sdk-node',
+});
diff --git a/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-sdk-node/start-otel-proxy.mjs b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-sdk-node/start-otel-proxy.mjs
new file mode 100644
index 000000000000..ecbbbabea624
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-sdk-node/start-otel-proxy.mjs
@@ -0,0 +1,6 @@
+import { startProxyServer } from '@sentry-internal/test-utils';
+
+startProxyServer({
+  port: 3032,
+  proxyServerName: 'node-core-express-otel-v1-sdk-node-otel',
+});
diff --git a/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-sdk-node/tests/errors.test.ts b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-sdk-node/tests/errors.test.ts
new file mode 100644
index 000000000000..7377bd3d91ce
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-sdk-node/tests/errors.test.ts
@@ -0,0 +1,63 @@
+import { expect, test } from '@playwright/test';
+import { waitForError, waitForTransaction } from '@sentry-internal/test-utils';
+
+test('Sends correct error event', async ({ baseURL }) => {
+  const errorEventPromise = waitForError('node-core-express-otel-v1-sdk-node', event => {
+    return !event.type && event.exception?.values?.[0]?.value === 'This is an exception with id 123';
+  });
+
+  await fetch(`${baseURL}/test-exception/123`);
+
+  const errorEvent = await errorEventPromise;
+
+  expect(errorEvent.exception?.values).toHaveLength(1);
+  expect(errorEvent.exception?.values?.[0]?.value).toBe('This is an exception with id 123');
+
+  expect(errorEvent.request).toEqual({
+    method: 'GET',
+    cookies: {},
+    headers: expect.any(Object),
+    url: 'http://localhost:3030/test-exception/123',
+  });
+
+  expect(errorEvent.transaction).toEqual('GET /test-exception/123');
+
+  expect(errorEvent.contexts?.trace).toEqual({
+    trace_id: expect.stringMatching(/[a-f0-9]{32}/),
+    span_id: expect.stringMatching(/[a-f0-9]{16}/),
+  });
+});
+
+test('Errors do not leak between requests', async ({ baseURL }) => {
+  // Set up promises to capture errors for both requests
+  const firstErrorPromise = waitForError('node-core-express-otel-v1-sdk-node', event => {
+    return !event.type && event.exception?.values?.[0]?.value === 'This is an exception with id 111';
+  });
+
+  const secondErrorPromise = waitForError('node-core-express-otel-v1-sdk-node', event => {
+    return !event.type && event.exception?.values?.[0]?.value === 'This is an exception with id 222';
+  });
+
+  // Make first error request
+  await fetch(`${baseURL}/test-exception/111`);
+
+  // Make second error request
+  await fetch(`${baseURL}/test-exception/222`);
+
+  // Wait for both error events to be captured
+  const [firstError, secondError] = await Promise.all([firstErrorPromise, secondErrorPromise]);
+
+  // Verify first error has correct data and doesn't contain data from second error
+  expect(firstError.exception?.values?.[0]?.value).toBe('This is an exception with id 111');
+  expect(firstError.transaction).toEqual('GET /test-exception/111');
+  expect(firstError.request?.url).toBe('http://localhost:3030/test-exception/111');
+
+  // Verify second error has correct data and doesn't contain data from first error
+  expect(secondError.exception?.values?.[0]?.value).toBe('This is an exception with id 222');
+  expect(secondError.transaction).toEqual('GET /test-exception/222');
+  expect(secondError.request?.url).toBe('http://localhost:3030/test-exception/222');
+
+  // Verify errors have different trace contexts (no leakage)
+  expect(firstError.contexts?.trace?.trace_id).not.toEqual(secondError.contexts?.trace?.trace_id);
+  expect(firstError.contexts?.trace?.span_id).not.toEqual(secondError.contexts?.trace?.span_id);
+});
diff --git a/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-sdk-node/tests/transactions.test.ts b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-sdk-node/tests/transactions.test.ts
new file mode 100644
index 000000000000..6141261d8954
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-sdk-node/tests/transactions.test.ts
@@ -0,0 +1,88 @@
+import { expect, test } from '@playwright/test';
+import { waitForPlainRequest, waitForTransaction } from '@sentry-internal/test-utils';
+
+test('Sends an API route transaction', async ({ baseURL }) => {
+  const pageloadTransactionEventPromise = waitForTransaction('node-core-express-otel-v1-sdk-node', transactionEvent => {
+    return (
+      transactionEvent?.contexts?.trace?.op === 'http.server' &&
+      transactionEvent?.transaction === 'GET /test-transaction'
+    );
+  });
+
+  // Ensure we also send data to the OTLP endpoint
+  const otelPromise = waitForPlainRequest('node-core-express-otel-v1-sdk-node-otel', data => {
+    const json = JSON.parse(data) as any;
+
+    return json.resourceSpans.length > 0;
+  });
+
+  await fetch(`${baseURL}/test-transaction`);
+
+  const transactionEvent = await pageloadTransactionEventPromise;
+
+  const otelData = await otelPromise;
+
+  // For now we do not test the actual shape of this, but only existence
+  expect(otelData).toBeDefined();
+
+  expect(transactionEvent.contexts?.trace).toEqual({
+    data: {
+      'sentry.source': 'url',
+      'sentry.origin': 'manual',
+      'sentry.op': 'http.server',
+      'sentry.sample_rate': 1,
+      url: 'http://localhost:3030/test-transaction',
+      'otel.kind': 'SERVER',
+      'http.response.status_code': 200,
+      'http.url': 'http://localhost:3030/test-transaction',
+      'http.host': 'localhost:3030',
+      'net.host.name': 'localhost',
+      'http.method': 'GET',
+      'http.scheme': 'http',
+      'http.target': '/test-transaction',
+      'http.user_agent': 'node',
+      'http.flavor': '1.1',
+      'net.transport': 'ip_tcp',
+      'net.host.ip': expect.any(String),
+      'net.host.port': expect.any(Number),
+      'net.peer.ip': expect.any(String),
+      'net.peer.port': expect.any(Number),
+      'http.status_code': 200,
+      'http.status_text': 'OK',
+    },
+    op: 'http.server',
+    span_id: expect.stringMatching(/[a-f0-9]{16}/),
+    status: 'ok',
+    trace_id: expect.stringMatching(/[a-f0-9]{32}/),
+    origin: 'manual',
+  });
+
+  expect(transactionEvent).toEqual(
+    expect.objectContaining({
+      transaction: 'GET /test-transaction',
+      type: 'transaction',
+      transaction_info: {
+        source: 'url',
+      },
+    }),
+  );
+});
+
+test('Sends an API route transaction for an errored route', async ({ baseURL }) => {
+  const transactionEventPromise = waitForTransaction('node-core-express-otel-v1-sdk-node', transactionEvent => {
+    return (
+      transactionEvent.contexts?.trace?.op === 'http.server' &&
+      transactionEvent.transaction === 'GET /test-exception/777' &&
+      transactionEvent.request?.url === 'http://localhost:3030/test-exception/777'
+    );
+  });
+
+  await fetch(`${baseURL}/test-exception/777`);
+
+  const transactionEvent = await transactionEventPromise;
+
+  expect(transactionEvent.contexts?.trace?.op).toEqual('http.server');
+  expect(transactionEvent.transaction).toEqual('GET /test-exception/777');
+  expect(transactionEvent.contexts?.trace?.status).toEqual('internal_error');
+  expect(transactionEvent.contexts?.trace?.data?.['http.status_code']).toEqual(500);
+});
diff --git a/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-sdk-node/tsconfig.json b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-sdk-node/tsconfig.json
new file mode 100644
index 000000000000..2887ec11a81d
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v1-sdk-node/tsconfig.json
@@ -0,0 +1,11 @@
+{
+  "compilerOptions": {
+    "types": ["node"],
+    "esModuleInterop": true,
+    "lib": ["es2018"],
+    "strict": true,
+    "outDir": "dist",
+    "skipLibCheck": true
+  },
+  "include": ["src/**/*.ts"]
+}
diff --git a/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-custom-sampler/.gitignore b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-custom-sampler/.gitignore
new file mode 100644
index 000000000000..686a0277246c
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-custom-sampler/.gitignore
@@ -0,0 +1,2 @@
+dist
+.vscode
diff --git a/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-custom-sampler/.npmrc b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-custom-sampler/.npmrc
new file mode 100644
index 000000000000..070f80f05092
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-custom-sampler/.npmrc
@@ -0,0 +1,2 @@
+@sentry:registry=http://127.0.0.1:4873
+@sentry-internal:registry=http://127.0.0.1:4873
diff --git a/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-custom-sampler/package.json b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-custom-sampler/package.json
new file mode 100644
index 000000000000..3b6c974f44b2
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-custom-sampler/package.json
@@ -0,0 +1,36 @@
+{
+  "name": "node-core-express-otel-v2-custom-sampler",
+  "version": "1.0.0",
+  "private": true,
+  "scripts": {
+    "build": "tsc",
+    "start": "node dist/app.js",
+    "test": "playwright test",
+    "clean": "npx rimraf node_modules pnpm-lock.yaml",
+    "test:build": "pnpm install && pnpm build",
+    "test:assert": "pnpm test"
+  },
+  "dependencies": {
+    "@opentelemetry/api": "^1.9.0",
+    "@opentelemetry/context-async-hooks": "^2.0.0",
+    "@opentelemetry/core": "^2.0.0",
+    "@opentelemetry/instrumentation": "^0.203.0",
+    "@opentelemetry/instrumentation-http": "^0.203.0",
+    "@opentelemetry/resources": "^2.0.0",
+    "@opentelemetry/sdk-trace-node": "^2.0.0",
+    "@opentelemetry/semantic-conventions": "^1.34.0",
+    "@sentry/node-core": "latest || *",
+    "@sentry/opentelemetry": "latest || *",
+    "@types/express": "4.17.17",
+    "@types/node": "^18.19.1",
+    "express": "4.19.2",
+    "typescript": "~5.0.0"
+  },
+  "devDependencies": {
+    "@playwright/test": "~1.53.2",
+    "@sentry-internal/test-utils": "link:../../../test-utils"
+  },
+  "volta": {
+    "extends": "../../package.json"
+  }
+}
diff --git a/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-custom-sampler/playwright.config.mjs b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-custom-sampler/playwright.config.mjs
new file mode 100644
index 000000000000..31f2b913b58b
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-custom-sampler/playwright.config.mjs
@@ -0,0 +1,7 @@
+import { getPlaywrightConfig } from '@sentry-internal/test-utils';
+
+const config = getPlaywrightConfig({
+  startCommand: `pnpm start`,
+});
+
+export default config;
diff --git a/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-custom-sampler/src/app.ts b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-custom-sampler/src/app.ts
new file mode 100644
index 000000000000..e5da185262ad
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-custom-sampler/src/app.ts
@@ -0,0 +1,51 @@
+import './instrument';
+
+import * as Sentry from '@sentry/node-core';
+import express from 'express';
+
+const PORT = 3030;
+const app = express();
+
+const wait = (duration: number) => {
+  return new Promise<void>(res => {
+    setTimeout(() => res(), duration);
+  });
+};
+
+app.get('/task', async (_req, res) => {
+  await Sentry.startSpan({ name: 'Long task', op: 'custom.op' }, async () => {
+    await wait(200);
+  });
+  res.send('ok');
+});
+
+app.get('/unsampled/task', async (_req, res) => {
+  await wait(200);
+  res.send('ok');
+});
+
+app.get('/test-error', async function (req, res) {
+  const exceptionId = Sentry.captureException(new Error('This is an error'));
+
+  await Sentry.flush(2000);
+
+  res.send({ exceptionId });
+});
+
+app.get('/test-exception/:id', function (req, _res) {
+  throw new Error(`This is an exception with id ${req.params.id}`);
+});
+
+app.use(function onError(err: unknown, req: any, res: any, next: any) {
+  // Explicitly capture the error with Sentry
+  Sentry.captureException(err);
+
+  // The error id is attached to `res.sentry` to be returned
+  // and optionally displayed to the user for support.
+  res.statusCode = 500;
+  res.end(res.sentry + '\n');
+});
+
+app.listen(PORT, () => {
+  console.log('App listening on ', PORT);
+});
diff --git a/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-custom-sampler/src/custom-sampler.ts b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-custom-sampler/src/custom-sampler.ts
new file mode 100644
index 000000000000..cbaaac57c8ea
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-custom-sampler/src/custom-sampler.ts
@@ -0,0 +1,31 @@
+import { Attributes, Context, Link, SpanKind } from '@opentelemetry/api';
+import { Sampler, SamplingResult } from '@opentelemetry/sdk-trace-node';
+import { wrapSamplingDecision } from '@sentry/opentelemetry';
+
+export class CustomSampler implements Sampler {
+  public shouldSample(
+    context: Context,
+    _traceId: string,
+    _spanName: string,
+    _spanKind: SpanKind,
+    attributes: Attributes,
+    _links: Link[],
+  ): SamplingResult {
+    const route = attributes['http.route'];
+    const target = attributes['http.target'];
+    const decision =
+      (typeof route === 'string' && route.includes('/unsampled')) ||
+      (typeof target === 'string' && target.includes('/unsampled'))
+        ? 0
+        : 1;
+    return wrapSamplingDecision({
+      decision,
+      context,
+      spanAttributes: attributes,
+    });
+  }
+
+  public toString(): string {
+    return CustomSampler.name;
+  }
+}
diff --git a/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-custom-sampler/src/instrument.ts b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-custom-sampler/src/instrument.ts
new file mode 100644
index 000000000000..b01601ad8910
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-custom-sampler/src/instrument.ts
@@ -0,0 +1,27 @@
+import { NodeTracerProvider } from '@opentelemetry/sdk-trace-node';
+import { HttpInstrumentation } from '@opentelemetry/instrumentation-http';
+import * as Sentry from '@sentry/node-core';
+import { SentryPropagator, SentrySpanProcessor } from '@sentry/opentelemetry';
+import { CustomSampler } from './custom-sampler';
+
+Sentry.init({
+  environment: 'qa', // dynamic sampling bias to keep transactions
+  dsn: process.env.E2E_TEST_DSN,
+  includeLocalVariables: true,
+  debug: !!process.env.DEBUG,
+  tunnel: `http://localhost:3031/`, // proxy server
+  tracesSampleRate: 1,
+  openTelemetryInstrumentations: [new HttpInstrumentation()],
+});
+
+const provider = new NodeTracerProvider({
+  sampler: new CustomSampler(),
+  spanProcessors: [new SentrySpanProcessor()],
+});
+
+provider.register({
+  propagator: new SentryPropagator(),
+  contextManager: new Sentry.SentryContextManager(),
+});
+
+Sentry.validateOpenTelemetrySetup();
diff --git a/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-custom-sampler/start-event-proxy.mjs b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-custom-sampler/start-event-proxy.mjs
new file mode 100644
index 000000000000..1c678218dde5
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-custom-sampler/start-event-proxy.mjs
@@ -0,0 +1,6 @@
+import { startEventProxyServer } from '@sentry-internal/test-utils';
+
+startEventProxyServer({
+  port: 3031,
+  proxyServerName: 'node-core-express-otel-v2-custom-sampler',
+});
diff --git a/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-custom-sampler/tests/errors.test.ts b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-custom-sampler/tests/errors.test.ts
new file mode 100644
index 000000000000..f2de2878ed55
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-custom-sampler/tests/errors.test.ts
@@ -0,0 +1,30 @@
+import { expect, test } from '@playwright/test';
+import { waitForError } from '@sentry-internal/test-utils';
+
+test('Sends correct error event', async ({ baseURL }) => {
+  const errorEventPromise = waitForError('node-core-express-otel-v2-custom-sampler', event => {
+    return !event.type && event.exception?.values?.[0]?.value === 'This is an exception with id 123';
+  });
+
+  await fetch(`${baseURL}/test-exception/123`);
+
+  const errorEvent = await errorEventPromise;
+
+  expect(errorEvent.exception?.values).toHaveLength(1);
+  expect(errorEvent.exception?.values?.[0]?.value).toBe('This is an exception with id 123');
+
+  expect(errorEvent.request).toEqual({
+    method: 'GET',
+    cookies: {},
+    headers: expect.any(Object),
+    url: 'http://localhost:3030/test-exception/123',
+  });
+
+  // For node-core without Express integration, transaction name is the actual URL
+  expect(errorEvent.transaction).toEqual('GET /test-exception/123');
+
+  expect(errorEvent.contexts?.trace).toEqual({
+    trace_id: expect.stringMatching(/[a-f0-9]{32}/),
+    span_id: expect.stringMatching(/[a-f0-9]{16}/),
+  });
+});
diff --git a/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-custom-sampler/tests/sampling.test.ts b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-custom-sampler/tests/sampling.test.ts
new file mode 100644
index 000000000000..134f9f22b429
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-custom-sampler/tests/sampling.test.ts
@@ -0,0 +1,95 @@
+import { expect, test } from '@playwright/test';
+import { waitForTransaction } from '@sentry-internal/test-utils';
+
+test('Sends a sampled API route transaction', async ({ baseURL }) => {
+  const transactionEventPromise = waitForTransaction('node-core-express-otel-v2-custom-sampler', transactionEvent => {
+    return transactionEvent?.contexts?.trace?.op === 'http.server' && transactionEvent?.transaction === 'GET /task';
+  });
+
+  await fetch(`${baseURL}/task`);
+
+  const transactionEvent = await transactionEventPromise;
+
+  expect(transactionEvent.contexts?.trace).toEqual({
+    span_id: expect.stringMatching(/[a-f0-9]{16}/),
+    trace_id: expect.stringMatching(/[a-f0-9]{32}/),
+    data: {
+      'sentry.source': 'url',
+      'sentry.op': 'http.server',
+      'sentry.origin': 'manual',
+      url: 'http://localhost:3030/task',
+      'otel.kind': 'SERVER',
+      'http.response.status_code': 200,
+      'http.url': 'http://localhost:3030/task',
+      'http.host': 'localhost:3030',
+      'net.host.name': 'localhost',
+      'http.method': 'GET',
+      'http.scheme': 'http',
+      'http.target': '/task',
+      'http.user_agent': 'node',
+      'http.flavor': '1.1',
+      'net.transport': 'ip_tcp',
+      'net.host.ip': expect.any(String),
+      'net.host.port': 3030,
+      'net.peer.ip': expect.any(String),
+      'net.peer.port': expect.any(Number),
+      'http.status_code': 200,
+      'http.status_text': 'OK',
+    },
+    origin: 'manual',
+    op: 'http.server',
+    status: 'ok',
+  });
+
+  expect(transactionEvent.spans?.length).toBe(1);
+
+  expect(transactionEvent.spans).toContainEqual({
+    span_id: expect.stringMatching(/[a-f0-9]{16}/),
+    trace_id: expect.stringMatching(/[a-f0-9]{32}/),
+    data: {
+      'sentry.origin': 'manual',
+      'sentry.op': 'custom.op',
+    },
+    description: 'Long task',
+    parent_span_id: expect.stringMatching(/[a-f0-9]{16}/),
+    start_timestamp: expect.any(Number),
+    timestamp: expect.any(Number),
+    status: 'ok',
+    op: 'custom.op',
+    origin: 'manual',
+  });
+});
+
+test('Does not send an unsampled API route transaction', async ({ baseURL }) => {
+  const unsampledTransactionEventPromise = waitForTransaction(
+    'node-core-express-otel-v2-custom-sampler',
+    transactionEvent => {
+      return (
+        transactionEvent?.contexts?.trace?.op === 'http.server' &&
+        transactionEvent?.transaction === 'GET /unsampled/task'
+      );
+    },
+  );
+
+  await fetch(`${baseURL}/unsampled/task`);
+
+  const promiseShouldNotResolve = () =>
+    new Promise<void>((resolve, reject) => {
+      const timeout = setTimeout(() => {
+        resolve(); // Test passes because promise did not resolve within timeout
+      }, 1000);
+
+      unsampledTransactionEventPromise.then(
+        () => {
+          clearTimeout(timeout);
+          reject(new Error('Promise should not have resolved'));
+        },
+        () => {
+          clearTimeout(timeout);
+          reject(new Error('Promise should not have been rejected'));
+        },
+      );
+    });
+
+  expect(promiseShouldNotResolve()).resolves.not.toThrow();
+});
diff --git a/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-custom-sampler/tsconfig.json b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-custom-sampler/tsconfig.json
new file mode 100644
index 000000000000..2887ec11a81d
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-custom-sampler/tsconfig.json
@@ -0,0 +1,11 @@
+{
+  "compilerOptions": {
+    "types": ["node"],
+    "esModuleInterop": true,
+    "lib": ["es2018"],
+    "strict": true,
+    "outDir": "dist",
+    "skipLibCheck": true
+  },
+  "include": ["src/**/*.ts"]
+}
diff --git a/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-sdk-node/.gitignore b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-sdk-node/.gitignore
new file mode 100644
index 000000000000..1521c8b7652b
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-sdk-node/.gitignore
@@ -0,0 +1 @@
+dist
diff --git a/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-sdk-node/.npmrc b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-sdk-node/.npmrc
new file mode 100644
index 000000000000..070f80f05092
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-sdk-node/.npmrc
@@ -0,0 +1,2 @@
+@sentry:registry=http://127.0.0.1:4873
+@sentry-internal:registry=http://127.0.0.1:4873
diff --git a/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-sdk-node/package.json b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-sdk-node/package.json
new file mode 100644
index 000000000000..9da336fdf8d1
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-sdk-node/package.json
@@ -0,0 +1,38 @@
+{
+  "name": "node-core-express-otel-v2-sdk-node",
+  "version": "1.0.0",
+  "private": true,
+  "scripts": {
+    "build": "tsc",
+    "start": "node dist/app.js",
+    "test": "playwright test",
+    "clean": "npx rimraf node_modules pnpm-lock.yaml",
+    "test:build": "pnpm install && pnpm build",
+    "test:assert": "pnpm test"
+  },
+  "dependencies": {
+    "@opentelemetry/api": "^1.9.0",
+    "@opentelemetry/context-async-hooks": "^2.0.0",
+    "@opentelemetry/core": "^2.0.0",
+    "@opentelemetry/instrumentation": "^0.203.0",
+    "@opentelemetry/instrumentation-http": "^0.203.0",
+    "@opentelemetry/resources": "^2.0.0",
+    "@opentelemetry/sdk-trace-node": "^2.0.0",
+    "@opentelemetry/semantic-conventions": "^1.34.0",
+    "@opentelemetry/sdk-node": "^0.203.0",
+    "@opentelemetry/exporter-trace-otlp-http": "^0.203.0",
+    "@sentry/node-core": "latest || *",
+    "@sentry/opentelemetry": "latest || *",
+    "@types/express": "4.17.17",
+    "@types/node": "^18.19.1",
+    "express": "4.19.2",
+    "typescript": "~5.0.0"
+  },
+  "devDependencies": {
+    "@playwright/test": "~1.53.2",
+    "@sentry-internal/test-utils": "link:../../../test-utils"
+  },
+  "volta": {
+    "extends": "../../package.json"
+  }
+}
diff --git a/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-sdk-node/playwright.config.mjs b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-sdk-node/playwright.config.mjs
new file mode 100644
index 000000000000..888e61cfb2dc
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-sdk-node/playwright.config.mjs
@@ -0,0 +1,34 @@
+import { getPlaywrightConfig } from '@sentry-internal/test-utils';
+
+const config = getPlaywrightConfig(
+  {
+    startCommand: `pnpm start`,
+  },
+  {
+    webServer: [
+      {
+        command: `node ./start-event-proxy.mjs`,
+        port: 3031,
+        stdout: 'pipe',
+        stderr: 'pipe',
+      },
+      {
+        command: `node ./start-otel-proxy.mjs`,
+        port: 3032,
+        stdout: 'pipe',
+        stderr: 'pipe',
+      },
+      {
+        command: 'pnpm start',
+        port: 3030,
+        stdout: 'pipe',
+        stderr: 'pipe',
+        env: {
+          PORT: 3030,
+        },
+      },
+    ],
+  },
+);
+
+export default config;
diff --git a/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-sdk-node/src/app.ts b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-sdk-node/src/app.ts
new file mode 100644
index 000000000000..69f55b25e6ce
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-sdk-node/src/app.ts
@@ -0,0 +1,55 @@
+import './instrument';
+
+// Other imports below
+import * as Sentry from '@sentry/node-core';
+import express from 'express';
+
+const app = express();
+const port = 3030;
+
+app.get('/test-success', function (req, res) {
+  res.send({ version: 'v1' });
+});
+
+app.get('/test-param/:param', function (req, res) {
+  res.send({ paramWas: req.params.param });
+});
+
+app.get('/test-transaction', function (req, res) {
+  Sentry.withActiveSpan(null, async () => {
+    Sentry.startSpan({ name: 'test-transaction', op: 'e2e-test' }, () => {
+      Sentry.startSpan({ name: 'test-span' }, () => undefined);
+    });
+
+    await Sentry.flush();
+
+    res.send({});
+  });
+});
+
+app.get('/test-error', async function (req, res) {
+  const exceptionId = Sentry.captureException(new Error('This is an error'));
+
+  await Sentry.flush(2000);
+
+  res.send({ exceptionId });
+});
+
+app.get('/test-exception/:id', function (req, _res) {
+  throw new Error(`This is an exception with id ${req.params.id}`);
+});
+
+app.use(function onError(err: unknown, req: any, res: any, next: any) {
+  // Explicitly capture the error with Sentry because @sentry/node-core doesn't have
+  // a way to capture errors from express like @sentry/node does.
+  res.sentry = Sentry.captureException(err);
+
+  // The error id is attached to `res.sentry` to be returned
+  // and optionally displayed to the user for support.
+  res.statusCode = 500;
+  res.end(res.sentry + '\n');
+});
+
+app.listen(port, () => {
+  console.log(`Example app listening on port ${port}`);
+});
diff --git a/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-sdk-node/src/instrument.ts b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-sdk-node/src/instrument.ts
new file mode 100644
index 000000000000..276b4f55ac73
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-sdk-node/src/instrument.ts
@@ -0,0 +1,35 @@
+const opentelemetry = require('@opentelemetry/sdk-node');
+const { OTLPTraceExporter } = require('@opentelemetry/exporter-trace-otlp-http');
+const Sentry = require('@sentry/node-core');
+const { HttpInstrumentation } = require('@opentelemetry/instrumentation-http');
+const { SentrySpanProcessor, SentryPropagator, SentrySampler } = require('@sentry/opentelemetry');
+
+const sentryClient = Sentry.init({
+  environment: 'qa', // dynamic sampling bias to keep transactions
+  dsn: process.env.E2E_TEST_DSN,
+  includeLocalVariables: true,
+  debug: true,
+  tunnel: `http://localhost:3031/`, // proxy server
+  tracesSampleRate: 1,
+});
+
+if (sentryClient) {
+  const sdk = new opentelemetry.NodeSDK({
+    sampler: new SentrySampler(sentryClient),
+    textMapPropagator: new SentryPropagator(),
+    contextManager: new Sentry.SentryContextManager(),
+    spanProcessors: [
+      new SentrySpanProcessor(),
+      new opentelemetry.node.BatchSpanProcessor(
+        new OTLPTraceExporter({
+          url: 'http://localhost:3032/',
+        }),
+      ),
+    ],
+    instrumentations: [new HttpInstrumentation()],
+  });
+
+  sdk.start();
+
+  Sentry.validateOpenTelemetrySetup();
+}
diff --git a/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-sdk-node/start-event-proxy.mjs b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-sdk-node/start-event-proxy.mjs
new file mode 100644
index 000000000000..5c5352234039
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-sdk-node/start-event-proxy.mjs
@@ -0,0 +1,6 @@
+import { startEventProxyServer } from '@sentry-internal/test-utils';
+
+startEventProxyServer({
+  port: 3031,
+  proxyServerName: 'node-core-express-otel-v2-sdk-node',
+});
diff --git a/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-sdk-node/start-otel-proxy.mjs b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-sdk-node/start-otel-proxy.mjs
new file mode 100644
index 000000000000..8875601e95bc
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-sdk-node/start-otel-proxy.mjs
@@ -0,0 +1,6 @@
+import { startProxyServer } from '@sentry-internal/test-utils';
+
+startProxyServer({
+  port: 3032,
+  proxyServerName: 'node-core-express-otel-v2-sdk-node-otel',
+});
diff --git a/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-sdk-node/tests/errors.test.ts b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-sdk-node/tests/errors.test.ts
new file mode 100644
index 000000000000..ec43573dc910
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-sdk-node/tests/errors.test.ts
@@ -0,0 +1,63 @@
+import { expect, test } from '@playwright/test';
+import { waitForError } from '@sentry-internal/test-utils';
+
+test('Sends correct error event', async ({ baseURL }) => {
+  const errorEventPromise = waitForError('node-core-express-otel-v2-sdk-node', event => {
+    return !event.type && event.exception?.values?.[0]?.value === 'This is an exception with id 123';
+  });
+
+  await fetch(`${baseURL}/test-exception/123`);
+
+  const errorEvent = await errorEventPromise;
+
+  expect(errorEvent.exception?.values).toHaveLength(1);
+  expect(errorEvent.exception?.values?.[0]?.value).toBe('This is an exception with id 123');
+
+  expect(errorEvent.request).toEqual({
+    method: 'GET',
+    cookies: {},
+    headers: expect.any(Object),
+    url: 'http://localhost:3030/test-exception/123',
+  });
+
+  expect(errorEvent.transaction).toEqual('GET /test-exception/123');
+
+  expect(errorEvent.contexts?.trace).toEqual({
+    trace_id: expect.stringMatching(/[a-f0-9]{32}/),
+    span_id: expect.stringMatching(/[a-f0-9]{16}/),
+  });
+});
+
+test('Errors do not leak between requests', async ({ baseURL }) => {
+  // Set up promises to capture errors for both requests
+  const firstErrorPromise = waitForError('node-core-express-otel-v2-sdk-node', event => {
+    return !event.type && event.exception?.values?.[0]?.value === 'This is an exception with id 111';
+  });
+
+  const secondErrorPromise = waitForError('node-core-express-otel-v2-sdk-node', event => {
+    return !event.type && event.exception?.values?.[0]?.value === 'This is an exception with id 222';
+  });
+
+  // Make first error request
+  await fetch(`${baseURL}/test-exception/111`);
+
+  // Make second error request
+  await fetch(`${baseURL}/test-exception/222`);
+
+  // Wait for both error events to be captured
+  const [firstError, secondError] = await Promise.all([firstErrorPromise, secondErrorPromise]);
+
+  // Verify first error has correct data and doesn't contain data from second error
+  expect(firstError.exception?.values?.[0]?.value).toBe('This is an exception with id 111');
+  expect(firstError.transaction).toEqual('GET /test-exception/111');
+  expect(firstError.request?.url).toBe('http://localhost:3030/test-exception/111');
+
+  // Verify second error has correct data and doesn't contain data from first error
+  expect(secondError.exception?.values?.[0]?.value).toBe('This is an exception with id 222');
+  expect(secondError.transaction).toEqual('GET /test-exception/222');
+  expect(secondError.request?.url).toBe('http://localhost:3030/test-exception/222');
+
+  // Verify errors have different trace contexts (no leakage)
+  expect(firstError.contexts?.trace?.trace_id).not.toEqual(secondError.contexts?.trace?.trace_id);
+  expect(firstError.contexts?.trace?.span_id).not.toEqual(secondError.contexts?.trace?.span_id);
+});
diff --git a/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-sdk-node/tests/transactions.test.ts b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-sdk-node/tests/transactions.test.ts
new file mode 100644
index 000000000000..08c8f80cd9f0
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-sdk-node/tests/transactions.test.ts
@@ -0,0 +1,88 @@
+import { expect, test } from '@playwright/test';
+import { waitForPlainRequest, waitForTransaction } from '@sentry-internal/test-utils';
+
+test('Sends an API route transaction', async ({ baseURL }) => {
+  const pageloadTransactionEventPromise = waitForTransaction('node-core-express-otel-v2-sdk-node', transactionEvent => {
+    return (
+      transactionEvent?.contexts?.trace?.op === 'http.server' &&
+      transactionEvent?.transaction === 'GET /test-transaction'
+    );
+  });
+
+  // Ensure we also send data to the OTLP endpoint
+  const otelPromise = waitForPlainRequest('node-core-express-otel-v2-sdk-node-otel', data => {
+    const json = JSON.parse(data) as any;
+
+    return json.resourceSpans.length > 0;
+  });
+
+  await fetch(`${baseURL}/test-transaction`);
+
+  const transactionEvent = await pageloadTransactionEventPromise;
+
+  const otelData = await otelPromise;
+
+  // For now we do not test the actual shape of this, but only existence
+  expect(otelData).toBeDefined();
+
+  expect(transactionEvent.contexts?.trace).toEqual({
+    data: {
+      'sentry.source': 'url',
+      'sentry.origin': 'manual',
+      'sentry.op': 'http.server',
+      'sentry.sample_rate': 1,
+      url: 'http://localhost:3030/test-transaction',
+      'otel.kind': 'SERVER',
+      'http.response.status_code': 200,
+      'http.url': 'http://localhost:3030/test-transaction',
+      'http.host': 'localhost:3030',
+      'net.host.name': 'localhost',
+      'http.method': 'GET',
+      'http.scheme': 'http',
+      'http.target': '/test-transaction',
+      'http.user_agent': 'node',
+      'http.flavor': '1.1',
+      'net.transport': 'ip_tcp',
+      'net.host.ip': expect.any(String),
+      'net.host.port': expect.any(Number),
+      'net.peer.ip': expect.any(String),
+      'net.peer.port': expect.any(Number),
+      'http.status_code': 200,
+      'http.status_text': 'OK',
+    },
+    op: 'http.server',
+    span_id: expect.stringMatching(/[a-f0-9]{16}/),
+    status: 'ok',
+    trace_id: expect.stringMatching(/[a-f0-9]{32}/),
+    origin: 'manual',
+  });
+
+  expect(transactionEvent).toEqual(
+    expect.objectContaining({
+      transaction: 'GET /test-transaction',
+      type: 'transaction',
+      transaction_info: {
+        source: 'url',
+      },
+    }),
+  );
+});
+
+test('Sends an API route transaction for an errored route', async ({ baseURL }) => {
+  const transactionEventPromise = waitForTransaction('node-core-express-otel-v2-sdk-node', transactionEvent => {
+    return (
+      transactionEvent.contexts?.trace?.op === 'http.server' &&
+      transactionEvent.transaction === 'GET /test-exception/777' &&
+      transactionEvent.request?.url === 'http://localhost:3030/test-exception/777'
+    );
+  });
+
+  await fetch(`${baseURL}/test-exception/777`);
+
+  const transactionEvent = await transactionEventPromise;
+
+  expect(transactionEvent.contexts?.trace?.op).toEqual('http.server');
+  expect(transactionEvent.transaction).toEqual('GET /test-exception/777');
+  expect(transactionEvent.contexts?.trace?.status).toEqual('internal_error');
+  expect(transactionEvent.contexts?.trace?.data?.['http.status_code']).toEqual(500);
+});
diff --git a/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-sdk-node/tsconfig.json b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-sdk-node/tsconfig.json
new file mode 100644
index 000000000000..2887ec11a81d
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/node-core-express-otel-v2-sdk-node/tsconfig.json
@@ -0,0 +1,11 @@
+{
+  "compilerOptions": {
+    "types": ["node"],
+    "esModuleInterop": true,
+    "lib": ["es2018"],
+    "strict": true,
+    "outDir": "dist",
+    "skipLibCheck": true
+  },
+  "include": ["src/**/*.ts"]
+}