diff --git a/packages/deno/package.json b/packages/deno/package.json
index b17f360b8a03..27d4b66e65c0 100644
--- a/packages/deno/package.json
+++ b/packages/deno/package.json
@@ -24,6 +24,7 @@
     "/build"
   ],
   "dependencies": {
+    "@opentelemetry/api": "^1.9.0",
     "@sentry/core": "10.9.0"
   },
   "scripts": {
diff --git a/packages/deno/src/index.ts b/packages/deno/src/index.ts
index 12bcedc35270..3b22fc8b1135 100644
--- a/packages/deno/src/index.ts
+++ b/packages/deno/src/index.ts
@@ -101,3 +101,4 @@ export { normalizePathsIntegration } from './integrations/normalizepaths';
 export { contextLinesIntegration } from './integrations/contextlines';
 export { denoCronIntegration } from './integrations/deno-cron';
 export { breadcrumbsIntegration } from './integrations/breadcrumbs';
+export { vercelAIIntegration } from './integrations/vercelai';
diff --git a/packages/deno/src/integrations/vercelai.ts b/packages/deno/src/integrations/vercelai.ts
new file mode 100644
index 000000000000..442818060124
--- /dev/null
+++ b/packages/deno/src/integrations/vercelai.ts
@@ -0,0 +1,51 @@
+/**
+ * This is a copy of the Vercel AI integration from the cloudflare SDK.
+ *
+ * The only difference is that it does not use `@opentelemetry/instrumentation`
+ * because Deno Workers do not support it in the same way.
+ *
+ * Therefore, we cannot automatically patch setting `experimental_telemetry: { isEnabled: true }`
+ * and users have to manually set this to get spans.
+ */
+
+import type { IntegrationFn } from '@sentry/core';
+import { addVercelAiProcessors, defineIntegration } from '@sentry/core';
+
+const INTEGRATION_NAME = 'VercelAI';
+
+const _vercelAIIntegration = (() => {
+  return {
+    name: INTEGRATION_NAME,
+    setup(client) {
+      addVercelAiProcessors(client);
+    },
+  };
+}) satisfies IntegrationFn;
+
+/**
+ * Adds Sentry tracing instrumentation for the [ai](https://www.npmjs.com/package/ai) library.
+ * This integration is not enabled by default, you need to manually add it.
+ *
+ * For more information, see the [`ai` documentation](https://sdk.vercel.ai/docs/ai-sdk-core/telemetry).
+ *
+ *  You need to enable collecting spans for a specific call by setting
+ * `experimental_telemetry.isEnabled` to `true` in the first argument of the function call.
+ *
+ * ```javascript
+ * const result = await generateText({
+ *   model: openai('gpt-4-turbo'),
+ *   experimental_telemetry: { isEnabled: true },
+ * });
+ * ```
+ *
+ * If you want to collect inputs and outputs for a specific call, you must specifically opt-in to each
+ * function call by setting `experimental_telemetry.recordInputs` and `experimental_telemetry.recordOutputs`
+ * to `true`.
+ *
+ * ```javascript
+ * const result = await generateText({
+ *  model: openai('gpt-4-turbo'),
+ *  experimental_telemetry: { isEnabled: true, recordInputs: true, recordOutputs: true },
+ * });
+ */
+export const vercelAIIntegration = defineIntegration(_vercelAIIntegration);
diff --git a/packages/deno/src/opentelemetry/tracer.ts b/packages/deno/src/opentelemetry/tracer.ts
new file mode 100644
index 000000000000..801badefa19f
--- /dev/null
+++ b/packages/deno/src/opentelemetry/tracer.ts
@@ -0,0 +1,110 @@
+import type { Context, Span, SpanOptions, Tracer, TracerProvider } from '@opentelemetry/api';
+import { SpanKind, trace } from '@opentelemetry/api';
+import {
+  SEMANTIC_ATTRIBUTE_SENTRY_OP,
+  SEMANTIC_ATTRIBUTE_SENTRY_ORIGIN,
+  startInactiveSpan,
+  startSpanManual,
+} from '@sentry/core';
+
+/**
+ * Set up a mock OTEL tracer to allow inter-op with OpenTelemetry emitted spans.
+ * This is not perfect but handles easy/common use cases.
+ */
+export function setupOpenTelemetryTracer(): void {
+  trace.setGlobalTracerProvider(new SentryDenoTraceProvider());
+}
+
+class SentryDenoTraceProvider implements TracerProvider {
+  private readonly _tracers: Map<string, Tracer> = new Map();
+
+  public getTracer(name: string, version?: string, options?: { schemaUrl?: string }): Tracer {
+    const key = `${name}@${version || ''}:${options?.schemaUrl || ''}`;
+    if (!this._tracers.has(key)) {
+      this._tracers.set(key, new SentryDenoTracer());
+    }
+
+    // eslint-disable-next-line @typescript-eslint/no-non-null-assertion
+    return this._tracers.get(key)!;
+  }
+}
+
+class SentryDenoTracer implements Tracer {
+  public startSpan(name: string, options?: SpanOptions): Span {
+    // Map OpenTelemetry SpanKind to Sentry operation
+    const op = this._mapSpanKindToOp(options?.kind);
+
+    return startInactiveSpan({
+      name,
+      ...options,
+      attributes: {
+        ...options?.attributes,
+        [SEMANTIC_ATTRIBUTE_SENTRY_ORIGIN]: 'manual',
+        [SEMANTIC_ATTRIBUTE_SENTRY_OP]: op,
+        'sentry.deno_tracer': true,
+      },
+    });
+  }
+
+  /**
+   * NOTE: This does not handle `context` being passed in. It will always put spans on the current scope.
+   */
+  public startActiveSpan<F extends (span: Span) => unknown>(name: string, fn: F): ReturnType<F>;
+  public startActiveSpan<F extends (span: Span) => unknown>(name: string, options: SpanOptions, fn: F): ReturnType<F>;
+  public startActiveSpan<F extends (span: Span) => unknown>(
+    name: string,
+    options: SpanOptions,
+    context: Context,
+    fn: F,
+  ): ReturnType<F>;
+  public startActiveSpan<F extends (span: Span) => unknown>(
+    name: string,
+    options: unknown,
+    context?: unknown,
+    fn?: F,
+  ): ReturnType<F> {
+    const opts = (typeof options === 'object' && options !== null ? options : {}) as SpanOptions;
+
+    // Map OpenTelemetry SpanKind to Sentry operation
+    const op = this._mapSpanKindToOp(opts.kind);
+
+    const spanOpts = {
+      name,
+      ...opts,
+      attributes: {
+        ...opts.attributes,
+        [SEMANTIC_ATTRIBUTE_SENTRY_ORIGIN]: 'manual',
+        [SEMANTIC_ATTRIBUTE_SENTRY_OP]: op,
+        'sentry.deno_tracer': true,
+      },
+    };
+
+    const callback = (
+      typeof options === 'function'
+        ? options
+        : typeof context === 'function'
+          ? context
+          : typeof fn === 'function'
+            ? fn
+            : () => {}
+    ) as F;
+
+    // In OTEL the semantic matches `startSpanManual` because spans are not auto-ended
+    return startSpanManual(spanOpts, callback) as ReturnType<F>;
+  }
+
+  private _mapSpanKindToOp(kind?: SpanKind): string {
+    switch (kind) {
+      case SpanKind.CLIENT:
+        return 'http.client';
+      case SpanKind.SERVER:
+        return 'http.server';
+      case SpanKind.PRODUCER:
+        return 'message.produce';
+      case SpanKind.CONSUMER:
+        return 'message.consume';
+      default:
+        return 'otel.span';
+    }
+  }
+}
diff --git a/packages/deno/src/sdk.ts b/packages/deno/src/sdk.ts
index 955417842f94..e670a86f50d6 100644
--- a/packages/deno/src/sdk.ts
+++ b/packages/deno/src/sdk.ts
@@ -16,6 +16,7 @@ import { denoContextIntegration } from './integrations/context';
 import { contextLinesIntegration } from './integrations/contextlines';
 import { globalHandlersIntegration } from './integrations/globalhandlers';
 import { normalizePathsIntegration } from './integrations/normalizepaths';
+import { setupOpenTelemetryTracer } from './opentelemetry/tracer';
 import { makeFetchTransport } from './transports';
 import type { DenoOptions } from './types';
 
@@ -97,5 +98,13 @@ export function init(options: DenoOptions = {}): Client {
     transport: options.transport || makeFetchTransport,
   };
 
-  return initAndBind(DenoClient, clientOptions);
+  const client = initAndBind(DenoClient, clientOptions);
+
+  // Set up OpenTelemetry compatibility to capture spans from libraries using @opentelemetry/api
+  // Note: This is separate from Deno's native OTEL support and doesn't capture auto-instrumented spans
+  if (!options.skipOpenTelemetrySetup) {
+    setupOpenTelemetryTracer();
+  }
+
+  return client;
 }
diff --git a/packages/deno/src/types.ts b/packages/deno/src/types.ts
index 422e561bb644..1659e7a635e1 100644
--- a/packages/deno/src/types.ts
+++ b/packages/deno/src/types.ts
@@ -23,6 +23,19 @@ export interface BaseDenoOptions {
   /** Sets an optional server name (device name) */
   serverName?: string;
 
+  /**
+   * The Deno SDK is not OpenTelemetry native, however, we set up some OpenTelemetry compatibility
+   * via a custom trace provider.
+   * This ensures that any spans emitted via `@opentelemetry/api` will be captured by Sentry.
+   * HOWEVER, big caveat: This does not handle custom context handling, it will always work off the current scope.
+   * This should be good enough for many, but not all integrations.
+   *
+   * If you want to opt-out of setting up the OpenTelemetry compatibility tracer, set this to `true`.
+   *
+   * @default false
+   */
+  skipOpenTelemetrySetup?: boolean;
+
   /** Callback that is executed when a fatal global error occurs. */
   onFatalError?(this: void, error: Error): void;
 }
diff --git a/packages/deno/test/opentelemetry.test.ts b/packages/deno/test/opentelemetry.test.ts
new file mode 100644
index 000000000000..57fc020a9219
--- /dev/null
+++ b/packages/deno/test/opentelemetry.test.ts
@@ -0,0 +1,145 @@
+import { assertEquals } from 'https://deno.land/std@0.212.0/assert/mod.ts';
+import { context, propagation, trace } from 'npm:@opentelemetry/api@1';
+import type { DenoClient } from '../build/esm/index.js';
+import { getCurrentScope, getGlobalScope, getIsolationScope, init, startSpan } from '../build/esm/index.js';
+
+function resetGlobals(): void {
+  getCurrentScope().clear();
+  getCurrentScope().setClient(undefined);
+  getIsolationScope().clear();
+  getGlobalScope().clear();
+}
+
+function cleanupOtel(): void {
+  // Disable all globally registered APIs
+  trace.disable();
+  context.disable();
+  propagation.disable();
+}
+
+function resetSdk(): void {
+  resetGlobals();
+  cleanupOtel();
+}
+
+Deno.test('should not capture spans emitted via @opentelemetry/api when skipOpenTelemetrySetup is true', async () => {
+  resetSdk();
+  const transactionEvents: any[] = [];
+
+  const client = init({
+    dsn: 'https://username@domain/123',
+    tracesSampleRate: 1,
+    skipOpenTelemetrySetup: true,
+    beforeSendTransaction: event => {
+      transactionEvents.push(event);
+      return null;
+    },
+  }) as DenoClient;
+
+  const tracer = trace.getTracer('test');
+  const span = tracer.startSpan('test');
+  span.end();
+
+  await client.flush();
+
+  tracer.startActiveSpan('test 2', { attributes: { 'test.attribute': 'test' } }, span2 => {
+    const span = tracer.startSpan('test 3', { attributes: { 'test.attribute': 'test2' } });
+    span.end();
+    span2.end();
+  });
+
+  await client.flush();
+
+  assertEquals(transactionEvents.length, 0);
+});
+
+Deno.test('should capture spans emitted via @opentelemetry/api', async () => {
+  resetSdk();
+  const transactionEvents: any[] = [];
+
+  const client = init({
+    dsn: 'https://username@domain/123',
+    tracesSampleRate: 1,
+    beforeSendTransaction: event => {
+      transactionEvents.push(event);
+      return null;
+    },
+  }) as DenoClient;
+
+  const tracer = trace.getTracer('test');
+  const span = tracer.startSpan('test');
+  span.end();
+
+  await client.flush();
+
+  tracer.startActiveSpan('test 2', { attributes: { 'test.attribute': 'test' } }, span2 => {
+    const span = tracer.startSpan('test 3', { attributes: { 'test.attribute': 'test2' } });
+    span.end();
+    span2.end();
+  });
+
+  await client.flush();
+
+  assertEquals(transactionEvents.length, 2);
+  const [transactionEvent, transactionEvent2] = transactionEvents;
+
+  assertEquals(transactionEvent?.spans?.length, 0);
+  assertEquals(transactionEvent?.transaction, 'test');
+  assertEquals(transactionEvent?.contexts?.trace?.data?.['sentry.deno_tracer'], true);
+  assertEquals(transactionEvent?.contexts?.trace?.data?.['sentry.origin'], 'manual');
+  assertEquals(transactionEvent?.contexts?.trace?.data?.['sentry.sample_rate'], 1);
+  assertEquals(transactionEvent?.contexts?.trace?.data?.['sentry.source'], 'custom');
+
+  assertEquals(transactionEvent2?.spans?.length, 1);
+  assertEquals(transactionEvent2?.transaction, 'test 2');
+  assertEquals(transactionEvent2?.contexts?.trace?.data?.['sentry.deno_tracer'], true);
+  assertEquals(transactionEvent2?.contexts?.trace?.data?.['sentry.origin'], 'manual');
+  assertEquals(transactionEvent2?.contexts?.trace?.data?.['sentry.sample_rate'], 1);
+  assertEquals(transactionEvent2?.contexts?.trace?.data?.['sentry.source'], 'custom');
+  assertEquals(transactionEvent2?.contexts?.trace?.data?.['test.attribute'], 'test');
+
+  const childSpan = transactionEvent2?.spans?.[0];
+  assertEquals(childSpan?.description, 'test 3');
+  assertEquals(childSpan?.data?.['sentry.deno_tracer'], true);
+  assertEquals(childSpan?.data?.['sentry.origin'], 'manual');
+  assertEquals(childSpan?.data?.['test.attribute'], 'test2');
+});
+
+Deno.test('opentelemetry spans should interop with Sentry spans', async () => {
+  resetSdk();
+  const transactionEvents: any[] = [];
+
+  const client = init({
+    dsn: 'https://username@domain/123',
+    tracesSampleRate: 1,
+    beforeSendTransaction: event => {
+      transactionEvents.push(event);
+      return null;
+    },
+  }) as DenoClient;
+
+  const tracer = trace.getTracer('test');
+
+  startSpan({ name: 'sentry span' }, () => {
+    const span = tracer.startSpan('otel span');
+    span.end();
+  });
+
+  await client.flush();
+
+  assertEquals(transactionEvents.length, 1);
+  const [transactionEvent] = transactionEvents;
+
+  assertEquals(transactionEvent?.spans?.length, 1);
+  assertEquals(transactionEvent?.transaction, 'sentry span');
+  assertEquals(transactionEvent?.contexts?.trace?.data?.['sentry.origin'], 'manual');
+  assertEquals(transactionEvent?.contexts?.trace?.data?.['sentry.sample_rate'], 1);
+  assertEquals(transactionEvent?.contexts?.trace?.data?.['sentry.source'], 'custom');
+  // Note: Sentry-created spans don't have the deno_tracer marker
+  assertEquals(transactionEvent?.contexts?.trace?.data?.['sentry.deno_tracer'], undefined);
+
+  const otelSpan = transactionEvent?.spans?.[0];
+  assertEquals(otelSpan?.description, 'otel span');
+  assertEquals(otelSpan?.data?.['sentry.deno_tracer'], true);
+  assertEquals(otelSpan?.data?.['sentry.origin'], 'manual');
+});