diff --git a/packages/core/src/tracing/utils.ts b/packages/core/src/tracing/utils.ts
index 5fa0a4b34420..6ca5594b3da6 100644
--- a/packages/core/src/tracing/utils.ts
+++ b/packages/core/src/tracing/utils.ts
@@ -1,29 +1,71 @@
 import type { Scope } from '../scope';
 import type { Span } from '../types-hoist/span';
 import { addNonEnumerableProperty } from '../utils/object';
+import { GLOBAL_OBJ } from '../utils/worldwide';
 
 const SCOPE_ON_START_SPAN_FIELD = '_sentryScope';
 const ISOLATION_SCOPE_ON_START_SPAN_FIELD = '_sentryIsolationScope';
 
+type ScopeWeakRef = { deref(): Scope | undefined } | Scope;
+
 type SpanWithScopes = Span & {
   [SCOPE_ON_START_SPAN_FIELD]?: Scope;
-  [ISOLATION_SCOPE_ON_START_SPAN_FIELD]?: Scope;
+  [ISOLATION_SCOPE_ON_START_SPAN_FIELD]?: ScopeWeakRef;
 };
 
+/** Wrap a scope with a WeakRef if available, falling back to a direct scope. */
+function wrapScopeWithWeakRef(scope: Scope): ScopeWeakRef {
+  try {
+    // @ts-expect-error - WeakRef is not available in all environments
+    const WeakRefClass = GLOBAL_OBJ.WeakRef;
+    if (typeof WeakRefClass === 'function') {
+      return new WeakRefClass(scope);
+    }
+  } catch {
+    // WeakRef not available or failed to create
+    // We'll fall back to a direct scope
+  }
+
+  return scope;
+}
+
+/** Try to unwrap a scope from a potential WeakRef wrapper. */
+function unwrapScopeFromWeakRef(scopeRef: ScopeWeakRef | undefined): Scope | undefined {
+  if (!scopeRef) {
+    return undefined;
+  }
+
+  if (typeof scopeRef === 'object' && 'deref' in scopeRef && typeof scopeRef.deref === 'function') {
+    try {
+      return scopeRef.deref();
+    } catch {
+      return undefined;
+    }
+  }
+
+  // Fallback to a direct scope
+  return scopeRef as Scope;
+}
+
 /** Store the scope & isolation scope for a span, which can the be used when it is finished. */
 export function setCapturedScopesOnSpan(span: Span | undefined, scope: Scope, isolationScope: Scope): void {
   if (span) {
-    addNonEnumerableProperty(span, ISOLATION_SCOPE_ON_START_SPAN_FIELD, isolationScope);
+    addNonEnumerableProperty(span, ISOLATION_SCOPE_ON_START_SPAN_FIELD, wrapScopeWithWeakRef(isolationScope));
+    // We don't wrap the scope with a WeakRef here because webkit aggressively garbage collects
+    // and scopes are not held in memory for long periods of time.
     addNonEnumerableProperty(span, SCOPE_ON_START_SPAN_FIELD, scope);
   }
 }
 
 /**
  * Grabs the scope and isolation scope off a span that were active when the span was started.
+ * If WeakRef was used and scopes have been garbage collected, returns undefined for those scopes.
  */
 export function getCapturedScopesOnSpan(span: Span): { scope?: Scope; isolationScope?: Scope } {
+  const spanWithScopes = span as SpanWithScopes;
+
   return {
-    scope: (span as SpanWithScopes)[SCOPE_ON_START_SPAN_FIELD],
-    isolationScope: (span as SpanWithScopes)[ISOLATION_SCOPE_ON_START_SPAN_FIELD],
+    scope: spanWithScopes[SCOPE_ON_START_SPAN_FIELD],
+    isolationScope: unwrapScopeFromWeakRef(spanWithScopes[ISOLATION_SCOPE_ON_START_SPAN_FIELD]),
   };
 }
diff --git a/packages/core/test/lib/tracing/utils.test.ts b/packages/core/test/lib/tracing/utils.test.ts
new file mode 100644
index 000000000000..63aba8c35529
--- /dev/null
+++ b/packages/core/test/lib/tracing/utils.test.ts
@@ -0,0 +1,244 @@
+import { describe, expect, it, vi } from 'vitest';
+import { Scope } from '../../../src/scope';
+import { getCapturedScopesOnSpan, setCapturedScopesOnSpan } from '../../../src/tracing/utils';
+import type { Span } from '../../../src/types-hoist/span';
+
+// Mock span object that implements the minimum needed interface
+function createMockSpan(): Span {
+  return {} as Span;
+}
+
+describe('tracing utils', () => {
+  describe('setCapturedScopesOnSpan / getCapturedScopesOnSpan', () => {
+    it('stores and retrieves scopes correctly', () => {
+      const span = createMockSpan();
+      const scope = new Scope();
+      const isolationScope = new Scope();
+
+      scope.setTag('test-scope', 'value1');
+      isolationScope.setTag('test-isolation-scope', 'value2');
+
+      setCapturedScopesOnSpan(span, scope, isolationScope);
+      const retrieved = getCapturedScopesOnSpan(span);
+
+      expect(retrieved.scope).toBe(scope);
+      expect(retrieved.isolationScope).toBe(isolationScope);
+      expect(retrieved.scope?.getScopeData().tags).toEqual({ 'test-scope': 'value1' });
+      expect(retrieved.isolationScope?.getScopeData().tags).toEqual({ 'test-isolation-scope': 'value2' });
+    });
+
+    it('handles undefined span gracefully in setCapturedScopesOnSpan', () => {
+      const scope = new Scope();
+      const isolationScope = new Scope();
+
+      expect(() => {
+        setCapturedScopesOnSpan(undefined, scope, isolationScope);
+      }).not.toThrow();
+    });
+
+    it('returns undefined scopes when span has no captured scopes', () => {
+      const span = createMockSpan();
+      const retrieved = getCapturedScopesOnSpan(span);
+
+      expect(retrieved.scope).toBeUndefined();
+      expect(retrieved.isolationScope).toBeUndefined();
+    });
+
+    it('uses WeakRef only for isolation scopes', () => {
+      const span = createMockSpan();
+      const scope = new Scope();
+      const isolationScope = new Scope();
+
+      setCapturedScopesOnSpan(span, scope, isolationScope);
+
+      // Check that only isolation scope is wrapped with WeakRef
+      // eslint-disable-next-line @typescript-eslint/no-explicit-any
+      const spanWithScopes = span as any;
+      expect(spanWithScopes._sentryScope).toBe(scope); // Regular scope stored directly
+      expect(spanWithScopes._sentryIsolationScope).toBeInstanceOf(WeakRef); // Isolation scope wrapped
+
+      // Verify we can still retrieve the scopes
+      const retrieved = getCapturedScopesOnSpan(span);
+      expect(retrieved.scope).toBe(scope);
+      expect(retrieved.isolationScope).toBe(isolationScope);
+    });
+
+    it('falls back to direct storage when WeakRef is not available', () => {
+      // Temporarily disable WeakRef
+      const originalWeakRef = globalThis.WeakRef;
+      // eslint-disable-next-line @typescript-eslint/no-explicit-any
+      (globalThis as any).WeakRef = undefined;
+
+      try {
+        const span = createMockSpan();
+        const scope = new Scope();
+        const isolationScope = new Scope();
+
+        setCapturedScopesOnSpan(span, scope, isolationScope);
+
+        // Check that both scopes are stored directly when WeakRef is not available
+        // eslint-disable-next-line @typescript-eslint/no-explicit-any
+        const spanWithScopes = span as any;
+        expect(spanWithScopes._sentryScope).toBe(scope); // Regular scope always stored directly
+        expect(spanWithScopes._sentryIsolationScope).toBe(isolationScope); // Isolation scope falls back to direct storage
+
+        // When WeakRef is available, ensure regular scope is not wrapped but isolation scope would be
+        if (originalWeakRef) {
+          expect(spanWithScopes._sentryScope).not.toBeInstanceOf(originalWeakRef);
+          expect(spanWithScopes._sentryIsolationScope).not.toBeInstanceOf(originalWeakRef);
+        }
+
+        // Verify we can still retrieve the scopes
+        const retrieved = getCapturedScopesOnSpan(span);
+        expect(retrieved.scope).toBe(scope);
+        expect(retrieved.isolationScope).toBe(isolationScope);
+      } finally {
+        // Restore WeakRef
+        // eslint-disable-next-line @typescript-eslint/no-explicit-any
+        (globalThis as any).WeakRef = originalWeakRef;
+      }
+    });
+
+    it('handles WeakRef deref returning undefined gracefully', () => {
+      const span = createMockSpan();
+      const scope = new Scope();
+      const isolationScope = new Scope();
+
+      setCapturedScopesOnSpan(span, scope, isolationScope);
+
+      // Mock WeakRef.deref to return undefined for isolation scope (simulating garbage collection)
+      // Regular scope is stored directly, so it should always be available
+      // eslint-disable-next-line @typescript-eslint/no-explicit-any
+      const spanWithScopes = span as any;
+      const mockIsolationScopeWeakRef = {
+        deref: vi.fn().mockReturnValue(undefined),
+      };
+
+      // Keep the regular scope as is (stored directly)
+      // Only replace the isolation scope with a mock WeakRef
+      spanWithScopes._sentryIsolationScope = mockIsolationScopeWeakRef;
+
+      const retrieved = getCapturedScopesOnSpan(span);
+      expect(retrieved.scope).toBe(scope); // Regular scope should still be available
+      expect(retrieved.isolationScope).toBeUndefined(); // Isolation scope should be undefined due to GC
+      expect(mockIsolationScopeWeakRef.deref).toHaveBeenCalled();
+    });
+
+    it('handles corrupted WeakRef objects gracefully', () => {
+      const span = createMockSpan();
+      const scope = new Scope();
+
+      // Set up a regular scope (stored directly) and a corrupted isolation scope WeakRef
+      // eslint-disable-next-line @typescript-eslint/no-explicit-any
+      const spanWithScopes = span as any;
+      spanWithScopes._sentryScope = scope; // Regular scope stored directly
+      spanWithScopes._sentryIsolationScope = {
+        deref: vi.fn().mockImplementation(() => {
+          throw new Error('WeakRef deref failed');
+        }),
+      };
+
+      const retrieved = getCapturedScopesOnSpan(span);
+      expect(retrieved.scope).toBe(scope); // Regular scope should still be available
+      expect(retrieved.isolationScope).toBeUndefined(); // Isolation scope should be undefined due to error
+    });
+
+    it('preserves scope data when using WeakRef', () => {
+      const span = createMockSpan();
+      const scope = new Scope();
+      const isolationScope = new Scope();
+
+      // Add various types of data to scopes
+      scope.setTag('string-tag', 'value');
+      scope.setTag('number-tag', 123);
+      scope.setTag('boolean-tag', true);
+      scope.setContext('test-context', { key: 'value' });
+      scope.setUser({ id: 'test-user' });
+
+      isolationScope.setExtra('extra-data', { complex: { nested: 'object' } });
+      isolationScope.setLevel('warning');
+
+      setCapturedScopesOnSpan(span, scope, isolationScope);
+      const retrieved = getCapturedScopesOnSpan(span);
+
+      // Verify all data is preserved
+      expect(retrieved.scope?.getScopeData().tags).toEqual({
+        'string-tag': 'value',
+        'number-tag': 123,
+        'boolean-tag': true,
+      });
+      expect(retrieved.scope?.getScopeData().contexts).toEqual({
+        'test-context': { key: 'value' },
+      });
+      expect(retrieved.scope?.getScopeData().user).toEqual({ id: 'test-user' });
+
+      expect(retrieved.isolationScope?.getScopeData().extra).toEqual({
+        'extra-data': { complex: { nested: 'object' } },
+      });
+      expect(retrieved.isolationScope?.getScopeData().level).toBe('warning');
+    });
+
+    it('handles multiple spans with different scopes', () => {
+      const span1 = createMockSpan();
+      const span2 = createMockSpan();
+
+      const scope1 = new Scope();
+      const scope2 = new Scope();
+      const isolationScope1 = new Scope();
+      const isolationScope2 = new Scope();
+
+      scope1.setTag('span', '1');
+      scope2.setTag('span', '2');
+      isolationScope1.setTag('isolation', '1');
+      isolationScope2.setTag('isolation', '2');
+
+      setCapturedScopesOnSpan(span1, scope1, isolationScope1);
+      setCapturedScopesOnSpan(span2, scope2, isolationScope2);
+
+      const retrieved1 = getCapturedScopesOnSpan(span1);
+      const retrieved2 = getCapturedScopesOnSpan(span2);
+
+      expect(retrieved1.scope?.getScopeData().tags).toEqual({ span: '1' });
+      expect(retrieved1.isolationScope?.getScopeData().tags).toEqual({ isolation: '1' });
+
+      expect(retrieved2.scope?.getScopeData().tags).toEqual({ span: '2' });
+      expect(retrieved2.isolationScope?.getScopeData().tags).toEqual({ isolation: '2' });
+
+      // Ensure they are different scope instances
+      expect(retrieved1.scope).not.toBe(retrieved2.scope);
+      expect(retrieved1.isolationScope).not.toBe(retrieved2.isolationScope);
+    });
+
+    it('handles span reuse correctly', () => {
+      const span = createMockSpan();
+
+      // First use
+      const scope1 = new Scope();
+      const isolationScope1 = new Scope();
+      scope1.setTag('first', 'use');
+      isolationScope1.setTag('first-isolation', 'use');
+
+      setCapturedScopesOnSpan(span, scope1, isolationScope1);
+      const retrieved1 = getCapturedScopesOnSpan(span);
+
+      expect(retrieved1.scope?.getScopeData().tags).toEqual({ first: 'use' });
+      expect(retrieved1.isolationScope?.getScopeData().tags).toEqual({ 'first-isolation': 'use' });
+
+      // Reuse with different scopes (overwrite)
+      const scope2 = new Scope();
+      const isolationScope2 = new Scope();
+      scope2.setTag('second', 'use');
+      isolationScope2.setTag('second-isolation', 'use');
+
+      setCapturedScopesOnSpan(span, scope2, isolationScope2);
+      const retrieved2 = getCapturedScopesOnSpan(span);
+
+      expect(retrieved2.scope?.getScopeData().tags).toEqual({ second: 'use' });
+      expect(retrieved2.isolationScope?.getScopeData().tags).toEqual({ 'second-isolation': 'use' });
+
+      // Should be the new scopes, not the old ones
+      expect(retrieved2.scope).toBe(scope2);
+      expect(retrieved2.isolationScope).toBe(isolationScope2);
+    });
+  });
+});