Merge branch 'main' of https://github.com/griffithlab/pVACtools_Intro_Course into main

Author: jhudsl-robot

docs/01-intro.md

@@ -3,6 +3,8 @@
 
 # Introduction
 
+This course is currently under development. The topics to be covered are outlined below.
+
 ## Motivation
 
 Identification of neoantigens is a critical step in predicting response to checkpoint blockade therapy and design of personalized cancer vaccines. This is a cross-disciplinary challenge, involving genomics, proteomics, immunology, and computational approaches. We have built a computational framework called pVACtools that, when paired with a well-established genomics pipeline, produces an end-to-end solution for neoantigen characterization. pVACtools supports identification of altered peptides from different mechanisms, including point mutations, in-frame and frameshift insertions and deletions, and gene fusions. Prediction of peptide:MHC binding is accomplished by supporting an ensemble of MHC Class I and II binding algorithms within a framework designed to facilitate the incorporation of additional algorithms. Prioritization of predicted peptides occurs by integrating diverse data, including mutant allele expression, peptide binding affinities, and determination whether a mutation is clonal or subclonal. Interactive visualization via a Web interface allows clinical users to efficiently generate, review, and interpret results, selecting candidate peptides for individual patient vaccine designs. Additional modules support design choices needed for competing vaccine delivery approaches. One such module optimizes peptide ordering to minimize junctional epitopes in DNA vector vaccines. Downstream analysis commands for synthetic long peptide vaccines are available to assess candidates for factors that influence peptide synthesis. All of the aforementioned steps are executed via a modular workflow consisting of tools for neoantigen prediction from somatic alterations (pVACseq and pVACfuse), prioritization, and selection using a graphical Web-based interface (pVACviz), and design of DNA vector–based vaccines (pVACvector) and synthetic long peptide vaccines. pVACtools is available at [https://www.pvactools.org](https://www.pvactools.org).

docs/index.html

@@ -161,7 +161,7 @@ <h1>
 </div>
 <div id="header">
 <h1 class="title">Introduction to pVACtools</h1>
-<p class="date"><em>May, 2023</em></p>
+<p class="date"><em>June, 2023</em></p>
 </div>
 <div id="about-this-course" class="section level1 unnumbered">
 <h1>About this Course</h1>

docs/index.md

@@ -1,6 +1,6 @@
 ---
 title: "Introduction to pVACtools"
-date: "May, 2023"
+date: "June, 2023"
 site: bookdown::bookdown_site
 documentclass: book
 bibliography: [book.bib, packages.bib]

docs/introduction.html

@@ -161,6 +161,7 @@ <h1>
 </div>
 <div id="introduction" class="section level1" number="1">
 <h1><span class="header-section-number">Chapter 1</span> Introduction</h1>
+<p>This course is currently under development. The topics to be covered are outlined below.</p>
 <div id="motivation" class="section level2" number="1.1">
 <h2><span class="header-section-number">1.1</span> Motivation</h2>
 <p>Identification of neoantigens is a critical step in predicting response to checkpoint blockade therapy and design of personalized cancer vaccines. This is a cross-disciplinary challenge, involving genomics, proteomics, immunology, and computational approaches. We have built a computational framework called pVACtools that, when paired with a well-established genomics pipeline, produces an end-to-end solution for neoantigen characterization. pVACtools supports identification of altered peptides from different mechanisms, including point mutations, in-frame and frameshift insertions and deletions, and gene fusions. Prediction of peptide:MHC binding is accomplished by supporting an ensemble of MHC Class I and II binding algorithms within a framework designed to facilitate the incorporation of additional algorithms. Prioritization of predicted peptides occurs by integrating diverse data, including mutant allele expression, peptide binding affinities, and determination whether a mutation is clonal or subclonal. Interactive visualization via a Web interface allows clinical users to efficiently generate, review, and interpret results, selecting candidate peptides for individual patient vaccine designs. Additional modules support design choices needed for competing vaccine delivery approaches. One such module optimizes peptide ordering to minimize junctional epitopes in DNA vector vaccines. Downstream analysis commands for synthetic long peptide vaccines are available to assess candidates for factors that influence peptide synthesis. All of the aforementioned steps are executed via a modular workflow consisting of tools for neoantigen prediction from somatic alterations (pVACseq and pVACfuse), prioritization, and selection using a graphical Web-based interface (pVACviz), and design of DNA vector–based vaccines (pVACvector) and synthetic long peptide vaccines. pVACtools is available at <a href="https://www.pvactools.org">https://www.pvactools.org</a>.</p>

docs/no_toc/01-intro.md

@@ -3,6 +3,8 @@
 
 # Introduction
 
+This course is currently under development. The topics to be covered are outlined below.
+
 ## Motivation
 
 Identification of neoantigens is a critical step in predicting response to checkpoint blockade therapy and design of personalized cancer vaccines. This is a cross-disciplinary challenge, involving genomics, proteomics, immunology, and computational approaches. We have built a computational framework called pVACtools that, when paired with a well-established genomics pipeline, produces an end-to-end solution for neoantigen characterization. pVACtools supports identification of altered peptides from different mechanisms, including point mutations, in-frame and frameshift insertions and deletions, and gene fusions. Prediction of peptide:MHC binding is accomplished by supporting an ensemble of MHC Class I and II binding algorithms within a framework designed to facilitate the incorporation of additional algorithms. Prioritization of predicted peptides occurs by integrating diverse data, including mutant allele expression, peptide binding affinities, and determination whether a mutation is clonal or subclonal. Interactive visualization via a Web interface allows clinical users to efficiently generate, review, and interpret results, selecting candidate peptides for individual patient vaccine designs. Additional modules support design choices needed for competing vaccine delivery approaches. One such module optimizes peptide ordering to minimize junctional epitopes in DNA vector vaccines. Downstream analysis commands for synthetic long peptide vaccines are available to assess candidates for factors that influence peptide synthesis. All of the aforementioned steps are executed via a modular workflow consisting of tools for neoantigen prediction from somatic alterations (pVACseq and pVACfuse), prioritization, and selection using a graphical Web-based interface (pVACviz), and design of DNA vector–based vaccines (pVACvector) and synthetic long peptide vaccines. pVACtools is available at [https://www.pvactools.org](https://www.pvactools.org).

docs/no_toc/index.html

@@ -161,7 +161,7 @@ <h1>
 </div>
 <div id="header">
 <h1 class="title">Introduction to pVACtools</h1>
-<p class="date"><em>May, 2023</em></p>
+<p class="date"><em>June, 2023</em></p>
 </div>
 <div id="about-this-course" class="section level1 unnumbered">
 <h1>About this Course</h1>

docs/no_toc/index.md

@@ -1,6 +1,6 @@
 ---
 title: "Introduction to pVACtools"
-date: "May, 2023"
+date: "June, 2023"
 site: bookdown::bookdown_site
 documentclass: book
 bibliography: [book.bib, packages.bib]

docs/no_toc/introduction.html

@@ -161,6 +161,7 @@ <h1>
 </div>
 <div id="introduction" class="section level1" number="1">
 <h1><span class="header-section-number">Chapter 1</span> Introduction</h1>
+<p>This course is currently under development. The topics to be covered are outlined below.</p>
 <div id="motivation" class="section level2" number="1.1">
 <h2><span class="header-section-number">1.1</span> Motivation</h2>
 <p>Identification of neoantigens is a critical step in predicting response to checkpoint blockade therapy and design of personalized cancer vaccines. This is a cross-disciplinary challenge, involving genomics, proteomics, immunology, and computational approaches. We have built a computational framework called pVACtools that, when paired with a well-established genomics pipeline, produces an end-to-end solution for neoantigen characterization. pVACtools supports identification of altered peptides from different mechanisms, including point mutations, in-frame and frameshift insertions and deletions, and gene fusions. Prediction of peptide:MHC binding is accomplished by supporting an ensemble of MHC Class I and II binding algorithms within a framework designed to facilitate the incorporation of additional algorithms. Prioritization of predicted peptides occurs by integrating diverse data, including mutant allele expression, peptide binding affinities, and determination whether a mutation is clonal or subclonal. Interactive visualization via a Web interface allows clinical users to efficiently generate, review, and interpret results, selecting candidate peptides for individual patient vaccine designs. Additional modules support design choices needed for competing vaccine delivery approaches. One such module optimizes peptide ordering to minimize junctional epitopes in DNA vector vaccines. Downstream analysis commands for synthetic long peptide vaccines are available to assess candidates for factors that influence peptide synthesis. All of the aforementioned steps are executed via a modular workflow consisting of tools for neoantigen prediction from somatic alterations (pVACseq and pVACfuse), prioritization, and selection using a graphical Web-based interface (pVACviz), and design of DNA vector–based vaccines (pVACvector) and synthetic long peptide vaccines. pVACtools is available at <a href="https://www.pvactools.org">https://www.pvactools.org</a>.</p>