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Provide updated information on how to download course data
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02-prerequisites.Rmd

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For this course, we have put together a set of input data for the HCC1395
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cell line. Data from this cell line is commonly used as test data in bioinformatics
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applications.
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applications. The input data consists of the following files:
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For pVACseq:
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- `annotated.expression.vcf.gz`: A somatic (tumor-normal) VCF and its tbi index file. The VCF has been
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annotated with VEP and has coverage and expression information added and.
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- `phased.vcf.gz`: A phased tumor-germline VCF and its tbi index file to provide information about
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in-phase proximal variants that might alter the predicted peptide sequence around a somatic
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mutation of interest
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- `optitype_normal_result.tsv`: A OptiType file with HLA allele typing predictions
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For pVACfuse:
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- `agfusion_results`: A AGFusion output directory with annotated fusion calls
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- `star-fusion.fusion_predictions.tsv`: A STARFusion prediction file with fusion read support
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and expression information
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General:
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- `Homo_sapiens.GRCh38.pep.all.fa.gz`: A reference proteome peptide FASTA to use
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for determining whether there are any reference matches of neoantigen candidates
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To download this data, please run the following command:
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```{r, engine = 'bash', eval = FALSE}
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TODO: download instructions
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wget
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https://raw.githubusercontent.com/griffithlab/pVACtools_Intro_Course/main/HCC1395_inputs.zip
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unzip HCC1395_inputs.zip
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```
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This course will not cover the required pre-processing steps for the pVACtools

HCC1395_inputs.zip

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