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docs/workflow.md

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@@ -83,13 +83,13 @@ for i in samples/*/; do bsub -oo $i/logs/regtools_compare_$tag.lsf regtools cis-
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## Statistical analysis
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### Make directory to store comparison results
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**Make directory to store comparison results**
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```bash
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mkdir -p compare_junctions/hist
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```
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### Run `compare_junctions_hist.py` on sample data
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**Run `compare_junctions_hist.py` on sample data**
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This script compares RegTools associations across all samples in a defined cohort to determine significance. By default, this script divides the input data into chunks of 50,000 variants in order to not max out memory for large cohorts. We provide three options for how to group case vs control groups with respect to a particular event (i.e. which samples do you consider to have the variant of interest vs. those that do not). The options are as follows:
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python3 compare_junctions_hist.py --tag <tag> --variant-grouping <parameter>
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```
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### Run `filter_and_BH.R` to adjust p values and filter results
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**Run `filter_and_BH.R` to adjust p values and filter results**
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This script filters significant results on junction support and junction type and then performs the Benjamini-Hochberg Procedure to apply multiple test correction to the data.
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