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Copy file name to clipboardExpand all lines: education/HADDOCK3/HADDOCK3-nanobody-antigen/index.md
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@@ -92,7 +92,7 @@ _and note the location of the extracted files in your system_. In it you should
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In this section we will prepare the PDB files of the nanobody and antigen for docking.
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Crystal structures of both the antibody and the antigen in their free forms are available from the
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[PDBe database](https://www.pdbe.org){:target="_blank"}. We will use [pdb-tools][link-pdbtools]{:target="_blank"} to perform several operations on the PDB files, such as selecting chains, renumbering residues, and creating ensembles. `pdb-tools` is already installed in the `haddock3` environment.
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[PDBe database](https://www.pdbe.org){:target="_blank"}. We will use [pdb-tools][link-pdbtools]{:target="_blank"} to perform several operations on the PDB files, such as selecting chains, renumbering residues, and creating ensembles. `pdb-tools` is already installed in the `haddock3` environment. For more information about the requirements for pdb files in HADDOCK3, please refer to the [HADDOCK3 user manual - structure requirements](https://www.bonvinlab.org/haddock3-user-manual/structure_requirements.html){:target="_blank"}.
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_**Note**_ that `pdb-tools` is also available as a [web service](https://wenmr.science.uu.nl/pdbtools/){:target="_blank"}.
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You can find all the contact maps in the `11_contactmap` directory of the run.
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## BONUS 1: design interface mutations in an nanobody-antigen complex with HADDOCK3
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## BONUS 1: design interface mutations in a nanobody-antigen complex with HADDOCK3
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HADDOCK3 can also be used to analyse and extract information from a nanobody-antigen complex.
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A benchmarking study of HADDOCK3 on a nanobody-antigen system has been published in [biorxiv](./){:target="_blank"}. Please refer to this publication for more information on the performance of HADDOCK3 on nanobody-antigen systems. If you use HADDOCK in your nanobody-focused research, please cite this publication.
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If you want to ask questions and receive feedback don't hesitate to contact us at the [Bioexcel HADDOCK forum][link-forum].
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If you want to ask questions and receive feedback don't hesitate to contact us at the [Bioexcel HADDOCK forum][link-forum].
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Another use case for nanobody antigen docking is the [HADDOCK nanobody-antigen example](https://github.com/haddocking/haddock3/tree/main/examples/docking-nanobody-antigen){:target="_blank"} in the HADDOCK3 GitHub repository, which contains similar workflows applied to a different system.
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