diff --git a/src/router/src/DispatcherFactory.php b/src/router/src/DispatcherFactory.php
index e666eaec9..70c840333 100644
--- a/src/router/src/DispatcherFactory.php
+++ b/src/router/src/DispatcherFactory.php
@@ -16,18 +16,20 @@ class DispatcherFactory extends BaseDispatcherFactory
 
     public function __construct(protected ContainerInterface $container)
     {
-        $this->routes = $container->get(RouteFileCollector::class)
-            ->getRouteFiles();
         $this->initAnnotationRoute(AnnotationCollector::list());
     }
 
-    public function initRoutes()
+    public function initRoutes(): void
     {
         $this->initialized = true;
 
         MiddlewareManager::$container = [];
 
-        foreach ($this->routes as $route) {
+        // Fetch route files at initialization time
+        // Ensures routes added via loadRoutesFrom() in service providers are included
+        $routes = $this->container->get(RouteFileCollector::class)->getRouteFiles();
+
+        foreach ($routes as $route) {
             if (file_exists($route)) {
                 require $route;
             }
diff --git a/tests/Router/DispatcherFactoryTest.php b/tests/Router/DispatcherFactoryTest.php
index 73278f189..a81a61e83 100644
--- a/tests/Router/DispatcherFactoryTest.php
+++ b/tests/Router/DispatcherFactoryTest.php
@@ -22,6 +22,15 @@
  */
 class DispatcherFactoryTest extends TestCase
 {
+    public static function setUpBeforeClass(): void
+    {
+        parent::setUpBeforeClass();
+
+        if (! defined('BASE_PATH')) {
+            define('BASE_PATH', dirname(__DIR__, 2));
+        }
+    }
+
     public function testGetRouter()
     {
         if (! defined('BASE_PATH')) {
@@ -66,6 +75,51 @@ public function testInitConfigRoute()
         $dispatcherFactory->initRoutes('http');
     }
 
+    /**
+     * Test that routes added to RouteFileCollector AFTER DispatcherFactory
+     * construction are still loaded when initRoutes() is called.
+     *
+     * This simulates the loadRoutesFrom() pattern where service providers
+     * add route files during boot(), after DispatcherFactory may have been
+     * constructed.
+     */
+    public function testRoutesAddedAfterConstructionAreLoaded()
+    {
+        if (! defined('BASE_PATH')) {
+            $this->markTestSkipped('skip it because DispatcherFactory in hyperf is dirty.');
+        }
+
+        /** @var MockInterface|RouteCollector */
+        $routeCollector = Mockery::mock(RouteCollector::class);
+
+        // Initial route from foo.php
+        $routeCollector->shouldReceive('get')->with('/foo', 'Handler::Foo')->once();
+
+        // Late-added route from late.php - this MUST be loaded
+        $routeCollector->shouldReceive('get')->with('/late', 'Handler::Late')->once();
+
+        // Create RouteFileCollector with only the initial route
+        $routeFileCollector = new RouteFileCollector([
+            __DIR__ . '/routes/foo.php',
+        ]);
+
+        $container = $this->getContainer([
+            HyperfRouteCollector::class => fn () => $routeCollector,
+            RouteFileCollector::class => fn () => $routeFileCollector,
+        ]);
+
+        // Create DispatcherFactory - this captures route files in constructor
+        $dispatcherFactory = new DispatcherFactory($container);
+        $container->define(Router::class, fn () => new Router($dispatcherFactory));
+
+        // Simulate service provider adding routes AFTER DispatcherFactory construction
+        // This is what loadRoutesFrom() does
+        $routeFileCollector->addRouteFile(__DIR__ . '/routes/late.php');
+
+        // Now trigger route loading - late.php should be included
+        $dispatcherFactory->getRouter('http');
+    }
+
     private function getContainer(array $bindings = []): Container
     {
         $container = new Container(
diff --git a/tests/Router/routes/late.php b/tests/Router/routes/late.php
new file mode 100644
index 000000000..35422ce04
--- /dev/null
+++ b/tests/Router/routes/late.php
@@ -0,0 +1,7 @@
+<?php
+
+declare(strict_types=1);
+
+use Hypervel\Router\Router;
+
+Router::get('/late', 'Handler::Late');