|
| 1 | +from collections import OrderedDict |
| 2 | + |
| 3 | +import numpy as np |
| 4 | +from ibllib.io.extractors import ephys_fpga |
| 5 | +from ibllib.dsp.utils import sync_timestamps |
| 6 | +from ibllib.plots import squares, vertical_lines |
| 7 | +from ibllib.pipes import tasks |
| 8 | + |
| 9 | +from ibllib.pipes.ephys_preprocessing import ( |
| 10 | + EphysRegisterRaw, EphysPulses, RawEphysQC, EphysAudio, EphysMtscomp, EphysVideoCompress, |
| 11 | + EphysCellsQc, EphysDLC, SpikeSorting) |
| 12 | + |
| 13 | +LASER_PULSE_DURATION_SECS = .5 |
| 14 | +LASER_PROBABILITY = .8 |
| 15 | +DISPLAY = True |
| 16 | + |
| 17 | + |
| 18 | +class EphysPassiveOptoTrials(tasks.Task): |
| 19 | + cpu = 1 |
| 20 | + io_charge = 90 |
| 21 | + level = 1 |
| 22 | + |
| 23 | + def _run(self): |
| 24 | + sync, sync_map = ephys_fpga.get_main_probe_sync(self.session_path) |
| 25 | + bpod = ephys_fpga.get_sync_fronts(sync, sync_map['bpod']) |
| 26 | + laser_ttl = ephys_fpga.get_sync_fronts(sync, sync_map['laser_ttl']) |
| 27 | + t_bpod = bpod['times'][bpod['polarities'] == 1] |
| 28 | + t_laser = laser_ttl['times'][laser_ttl['polarities'] == 1] |
| 29 | + _, _, ibpod, ilaser = sync_timestamps(t_bpod, t_laser, return_indices=True) |
| 30 | + |
| 31 | + if DISPLAY: |
| 32 | + for ch in np.arange(3): |
| 33 | + ch0 = ephys_fpga.get_sync_fronts(sync, 16 + ch) |
| 34 | + squares(ch0['times'], ch0['polarities'], yrange=[-.5 + ch, .5 + ch]) |
| 35 | + vertical_lines(t_bpod[ibpod], ymax=4) |
| 36 | + |
| 37 | + trial_starts = t_bpod |
| 38 | + trial_starts[ibpod] = t_laser[ilaser] |
| 39 | + ntrials = trial_starts.size |
| 40 | + |
| 41 | + laser_intervals = np.zeros((ntrials, 2)) * np.nan |
| 42 | + laser_intervals[ibpod, 0] = t_laser[ilaser] |
| 43 | + laser_intervals[ibpod, 1] = t_laser[ilaser] + LASER_PULSE_DURATION_SECS |
| 44 | + intervals = np.zeros((ntrials, 2)) * np.nan |
| 45 | + intervals[:, 0] = trial_starts |
| 46 | + intervals[:, 1] = np.r_[trial_starts[1:], np.nan] |
| 47 | + alf_path = self.session_path.joinpath('alf') |
| 48 | + alf_path.mkdir(parents=True, exist_ok=True) |
| 49 | + |
| 50 | + out_files = [ |
| 51 | + alf_path.joinpath("_ibl_trials.laser_probability.npy"), |
| 52 | + alf_path.joinpath("_ibl_trials.laser_intervals.npy"), |
| 53 | + alf_path.joinpath("_ibl_trials.intervals.npy"), |
| 54 | + ] |
| 55 | + np.save(alf_path.joinpath("_ibl_trials.laser_intervals.npy"), laser_intervals) |
| 56 | + np.save(alf_path.joinpath("_ibl_trials.laser_probability.npy"), trial_starts * 0 + LASER_PROBABILITY) |
| 57 | + np.save(alf_path.joinpath("_ibl_trials.intervals.npy"), intervals) |
| 58 | + |
| 59 | + return out_files |
| 60 | + |
| 61 | + |
| 62 | +class EphysPassiveOptoPipeline(tasks.Pipeline): |
| 63 | + label = __name__ |
| 64 | + |
| 65 | + def __init__(self, session_path=None, **kwargs): |
| 66 | + super(EphysPassiveOptoPipeline, self).__init__(session_path, **kwargs) |
| 67 | + tasks = OrderedDict() |
| 68 | + self.session_path = session_path |
| 69 | + # level 0 |
| 70 | + tasks["EphysRegisterRaw"] = EphysRegisterRaw(self.session_path) |
| 71 | + tasks["EphysPulses"] = EphysPulses(self.session_path) |
| 72 | + tasks["EphysRawQC"] = RawEphysQC(self.session_path) |
| 73 | + tasks["EphysAudio"] = EphysAudio(self.session_path) |
| 74 | + tasks["EphysMtscomp"] = EphysMtscomp(self.session_path) |
| 75 | + # level 1 |
| 76 | + tasks["SpikeSorting"] = SpikeSorting( |
| 77 | + self.session_path, parents=[tasks["EphysMtscomp"], tasks["EphysPulses"]]) |
| 78 | + tasks["EphysPassiveOptoTrials"] = EphysPassiveOptoTrials(self.session_path, parents=[tasks["EphysPulses"]]) |
| 79 | + tasks["EphysVideoCompress"] = EphysVideoCompress( |
| 80 | + self.session_path, parents=[tasks["EphysPulses"]]) |
| 81 | + # level 2 |
| 82 | + tasks["EphysCellsQc"] = EphysCellsQc(self.session_path, parents=[tasks["SpikeSorting"]]) |
| 83 | + tasks["EphysDLC"] = EphysDLC(self.session_path, parents=[tasks["EphysVideoCompress"]]) |
| 84 | + self.tasks = tasks |
| 85 | + |
| 86 | + |
| 87 | +# |
| 88 | +# from one.api import ONE |
| 89 | +# |
| 90 | +# one = ONE() |
| 91 | +# session_path = Path("/mnt/s0/Data/Subjects/KS056/2021-07-18/001") |
| 92 | +# |
| 93 | +# session_path = "/mnt/iblserver2/ephys_recordings/KS056/2021-07-18/001" |
| 94 | +# |
| 95 | +# pipeline = EphysPassiveOptoPipeline(session_path, one=one) |
| 96 | +# pipeline.run() |
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