> I just want to make a design experiment for RSM analysis where my variables are sugars (0-20%) and Agar (0.1-1.5%). > >  > > The results have some minus concentration. Could you tell me what should we do for the minus treatment? > _Originally posted by @Lia-UC in [#1705](https://github.com/jasp-stats/jasp-issues/issues/1705#issuecomment-2863536209)_