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@@ -334,7 +334,7 @@ SRR5427886.10866 chr14:53438632,+ SRR5427886.4746 singl
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* To avoid excessive memory usage and the imprecision inherent in floating-point values, a maximum of 10 alignments per read/fragment are analyzed by Genrich. Reads/fragments with more than 10 alignments within the `-s` threshold are subsampled based on the best alignment scores; in the case of ties, alignments appearing first in the SAM/BAM are favored.
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* The alignment score for a fragment (pair of reads) is equal to the sum of the reads' individual scores.
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* Properly paired alignments take precedence over unpaired alignments, regardless of the alignment scores.
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* As stated [above](#multimap), [Bowtie2](http://bowtie-bio.sourceforge.net/bowtie2/manual.shtml) reports secondary alignments in [`-k <int>` mode](http://bowtie-bio.sourceforge.net/bowtie2/manual.shtml#k-mode-search-for-one-or-more-alignments-report-each) or [`-a` mode](http://bowtie-bio.sourceforge.net/bowtie2/manual.shtml#a-mode-search-for-and-report-all-alignments). Note that a [software bug](https://github.com/BenLangmead/bowtie2/issues/202) causes errors with some secondary alignments that Bowtie2 reports; perhaps it will be fixed at some point. The short read aligner [BWA](http://bio-bwa.sourceforge.net/bwa.shtml) does not report secondary alignments.
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* As stated [above](#multimap), [Bowtie2](http://bowtie-bio.sourceforge.net/bowtie2/manual.shtml) reports secondary alignments in [`-k <int>` mode](http://bowtie-bio.sourceforge.net/bowtie2/manual.shtml#k-mode-search-for-one-or-more-alignments-report-each) or [`-a` mode](http://bowtie-bio.sourceforge.net/bowtie2/manual.shtml#a-mode-search-for-and-report-all-alignments). The short read aligner [BWA](http://bio-bwa.sourceforge.net/bwa.shtml) does not report secondary alignments.
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<br><br>
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### Unpaired alignments<a name="unpaired"></a>

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