diff --git a/ui-tests/environment.yml b/ui-tests/env1.yml
similarity index 61%
rename from ui-tests/environment.yml
rename to ui-tests/env1.yml
index 709db9ec..71f277bc 100644
--- a/ui-tests/environment.yml
+++ b/ui-tests/env1.yml
@@ -1,6 +1,6 @@
-name: jupyterlite-xeus-tests
+name: env1
 channels:
-  - https://repo.mamba.pm/emscripten-forge
+  - https://repo.prefix.dev/emscripten-forge-dev
   - conda-forge
 dependencies:
   - xeus-python
diff --git a/ui-tests/env2.yml b/ui-tests/env2.yml
new file mode 100644
index 00000000..7b1542da
--- /dev/null
+++ b/ui-tests/env2.yml
@@ -0,0 +1,6 @@
+name: env2
+channels:
+  - https://repo.prefix.dev/emscripten-forge-dev
+  - conda-forge
+dependencies:
+  - xeus-python
diff --git a/ui-tests/jupyter_lite_config.json b/ui-tests/jupyter_lite_config.json
index 11bf9467..4a199ee5 100644
--- a/ui-tests/jupyter_lite_config.json
+++ b/ui-tests/jupyter_lite_config.json
@@ -1,5 +1,8 @@
 {
   "LiteBuildConfig": {
     "output_dir": "dist"
+  },
+  "XeusAddon": {
+    "environment_file": ["env1.yml", "env2.yml"]
   }
 }
diff --git a/ui-tests/tests/jupyterlite_xeus.spec.ts b/ui-tests/tests/jupyterlite_xeus.spec.ts
index 95f88817..159c733d 100644
--- a/ui-tests/tests/jupyterlite_xeus.spec.ts
+++ b/ui-tests/tests/jupyterlite_xeus.spec.ts
@@ -25,7 +25,9 @@ test.describe('General Tests', () => {
   test('xeus-python should execute some code', async ({ page }) => {
     await page.goto('lab/index.html');
 
-    const xpython = page.locator('[title="Python 3.11 (XPython)"]').first();
+    const xpython = page
+      .locator('[title="Python 3.13 (XPython) [env1]"]')
+      .first();
     await xpython.click();
 
     // Wait for kernel to be idle
@@ -44,11 +46,40 @@ test.describe('General Tests', () => {
     );
   });
 
+  test('should support the same kernel from a second environment', async ({
+    page
+  }) => {
+    await page.goto('lab/index.html');
+
+    const xpython = page
+      .locator('[title="Python 3.13 (XPython) [env2]"]')
+      .first();
+    await xpython.click();
+
+    // Wait for kernel to be idle
+    await page.locator('#jp-main-statusbar').getByText('Idle').waitFor();
+
+    // xeus-python from env2 does not have bqplot installed.
+    await page.notebook.addCell('code', 'import bqplot');
+    await page.notebook.runCell(1);
+
+    // Wait for kernel to be idle
+    await page.locator('#jp-main-statusbar').getByText('Idle').waitFor();
+
+    const cell = await page.notebook.getCellOutput(1);
+
+    expect(await cell?.screenshot()).toMatchSnapshot(
+      'jupyter-xeus-execute-env2.png'
+    );
+  });
+
   test('the kernel should have access to the file system', async ({ page }) => {
     await page.goto('lab/index.html');
 
     // Create a Python notebook
-    const xpython = page.locator('[title="Python 3.11 (XPython)"]').first();
+    const xpython = page
+      .locator('[title="Python 3.13 (XPython) [env1]"]')
+      .first();
     await xpython.click();
 
     await page.notebook.save();
diff --git a/ui-tests/tests/jupyterlite_xeus.spec.ts-snapshots/jupyter-xeus-execute-env2-crossoriginisolated-linux.png b/ui-tests/tests/jupyterlite_xeus.spec.ts-snapshots/jupyter-xeus-execute-env2-crossoriginisolated-linux.png
new file mode 100644
index 00000000..7ca50447
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diff --git a/ui-tests/tests/jupyterlite_xeus.spec.ts-snapshots/jupyter-xeus-execute-env2-default-linux.png b/ui-tests/tests/jupyterlite_xeus.spec.ts-snapshots/jupyter-xeus-execute-env2-default-linux.png
new file mode 100644
index 00000000..7ca50447
Binary files /dev/null and b/ui-tests/tests/jupyterlite_xeus.spec.ts-snapshots/jupyter-xeus-execute-env2-default-linux.png differ
diff --git a/ui-tests/tests/jupyterlite_xeus.spec.ts-snapshots/jupyter-xeus-launcher-crossoriginisolated-linux.png b/ui-tests/tests/jupyterlite_xeus.spec.ts-snapshots/jupyter-xeus-launcher-crossoriginisolated-linux.png
index fdc55792..65dbd619 100644
Binary files a/ui-tests/tests/jupyterlite_xeus.spec.ts-snapshots/jupyter-xeus-launcher-crossoriginisolated-linux.png and b/ui-tests/tests/jupyterlite_xeus.spec.ts-snapshots/jupyter-xeus-launcher-crossoriginisolated-linux.png differ
diff --git a/ui-tests/tests/jupyterlite_xeus.spec.ts-snapshots/jupyter-xeus-launcher-default-linux.png b/ui-tests/tests/jupyterlite_xeus.spec.ts-snapshots/jupyter-xeus-launcher-default-linux.png
index fdc55792..65dbd619 100644
Binary files a/ui-tests/tests/jupyterlite_xeus.spec.ts-snapshots/jupyter-xeus-launcher-default-linux.png and b/ui-tests/tests/jupyterlite_xeus.spec.ts-snapshots/jupyter-xeus-launcher-default-linux.png differ