diff --git a/conformance/tests/httproute-hostname-precedence.go b/conformance/tests/httproute-hostname-precedence.go
new file mode 100644
index 0000000000..a2c41d9d14
--- /dev/null
+++ b/conformance/tests/httproute-hostname-precedence.go
@@ -0,0 +1,81 @@
+/*
+Copyright 2025 The Kubernetes Authors.
+
+Licensed under the Apache License, Version 2.0 (the "License");
+you may not use this file except in compliance with the License.
+You may obtain a copy of the License at
+
+    http://www.apache.org/licenses/LICENSE-2.0
+
+Unless required by applicable law or agreed to in writing, software
+distributed under the License is distributed on an "AS IS" BASIS,
+WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied.
+See the License for the specific language governing permissions and
+limitations under the License.
+*/
+
+package tests
+
+import (
+	"testing"
+
+	"k8s.io/apimachinery/pkg/types"
+
+	"sigs.k8s.io/gateway-api/conformance/utils/http"
+	"sigs.k8s.io/gateway-api/conformance/utils/kubernetes"
+	"sigs.k8s.io/gateway-api/conformance/utils/suite"
+	"sigs.k8s.io/gateway-api/pkg/features"
+)
+
+func init() {
+	ConformanceTests = append(ConformanceTests, HTTPRouteHostnamePrecedence)
+}
+
+var HTTPRouteHostnamePrecedence = suite.ConformanceTest{
+	ShortName:   "HTTPRouteHostnamePrecedence",
+	Description: "When a Gateway has both an HTTPRoute with a specified hostname and another without a hostname, the route with the specified hostname should take precedence",
+	Manifests:   []string{"tests/httproute-hostname-precedence.yaml"},
+	Features: []features.FeatureName{
+		features.SupportGateway,
+		features.SupportHTTPRoute,
+	},
+	Test: func(t *testing.T, suite *suite.ConformanceTestSuite) {
+		ns := "gateway-conformance-infra"
+		specificRouteNN := types.NamespacedName{Name: "hostname-precedence-with-specific", Namespace: ns}
+		nonSpecificRouteNN := types.NamespacedName{Name: "hostname-precedence-without-specific", Namespace: ns}
+		gwNN := types.NamespacedName{Name: "same-namespace", Namespace: ns}
+
+		gwAddr := kubernetes.GatewayAndHTTPRoutesMustBeAccepted(t, suite.Client, suite.TimeoutConfig, suite.ControllerName, kubernetes.NewGatewayRef(gwNN), specificRouteNN)
+		kubernetes.GatewayAndHTTPRoutesMustBeAccepted(t, suite.Client, suite.TimeoutConfig, suite.ControllerName, kubernetes.NewGatewayRef(gwNN), nonSpecificRouteNN)
+
+		kubernetes.HTTPRouteMustHaveResolvedRefsConditionsTrue(t, suite.Client, suite.TimeoutConfig, nonSpecificRouteNN, gwNN)
+		kubernetes.HTTPRouteMustHaveResolvedRefsConditionsTrue(t, suite.Client, suite.TimeoutConfig, specificRouteNN, gwNN)
+
+		expectedHeaders := map[string]string{
+			"test": "true",
+		}
+
+		testCases := []http.ExpectedResponse{
+			{
+				Request:   http.Request{Path: "/hostname-precedence", Host: "test.com"},
+				Response:  http.Response{Headers: expectedHeaders},
+				Namespace: ns,
+			},
+			{
+				Request:   http.Request{Path: "/hostname-precedence"},
+				Response:  http.Response{AbsentHeaders: []string{"test"}},
+				Namespace: ns,
+			},
+		}
+
+		for i := range testCases {
+			// Declare tc here to avoid loop variable
+			// reuse issues across parallel tests.
+			tc := testCases[i]
+			t.Run(tc.GetTestCaseName(i), func(t *testing.T) {
+				t.Parallel()
+				http.MakeRequestAndExpectEventuallyConsistentResponse(t, suite.RoundTripper, suite.TimeoutConfig, gwAddr, tc)
+			})
+		}
+	},
+}
diff --git a/conformance/tests/httproute-hostname-precedence.yaml b/conformance/tests/httproute-hostname-precedence.yaml
new file mode 100644
index 0000000000..2c6a2f9c1d
--- /dev/null
+++ b/conformance/tests/httproute-hostname-precedence.yaml
@@ -0,0 +1,41 @@
+apiVersion: gateway.networking.k8s.io/v1
+kind: HTTPRoute
+metadata:
+  name: hostname-precedence-without-specific
+  namespace: gateway-conformance-infra
+spec:
+  parentRefs:
+  - name: same-namespace
+  rules:
+  - matches:
+    - path:
+        type: PathPrefix
+        value: /hostname-precedence
+    backendRefs:
+    - name: infra-backend-v1
+      port: 8080
+---
+apiVersion: gateway.networking.k8s.io/v1
+kind: HTTPRoute
+metadata:
+  name: hostname-precedence-with-specific
+  namespace: gateway-conformance-infra
+spec:
+  parentRefs:
+  - name: same-namespace
+  hostnames:
+  - "test.com"
+  rules:
+  - matches:
+    - path:
+        type: PathPrefix
+        value: /hostname-precedence
+    filters:
+    - type: ResponseHeaderModifier
+      responseHeaderModifier:
+        add:
+        - name: test
+          value: "true"
+    backendRefs:
+    - name: infra-backend-v1
+      port: 8080