diff --git a/apps/E2E/src/Menu/specs/Menu.spec.win.ts b/apps/E2E/src/Menu/specs/Menu.spec.win.ts
index 8bbb4b807c..8e472fdd67 100644
--- a/apps/E2E/src/Menu/specs/Menu.spec.win.ts
+++ b/apps/E2E/src/Menu/specs/Menu.spec.win.ts
@@ -160,15 +160,15 @@ describe('Menu Functional Testing', () => {
       .toBeFalsy();
   });
 
-  it('Press "Tab" to navigate between MenuItems. Validate that focus switches correctly between MenuItems.', async () => {
+  it('Press "Tab" to navigate between MenuGroups. Validate that focus switches correctly between MenuGroups.', async () => {
     await MenuPageObject.openMenu();
 
-    await MenuPageObject.sendKeys(MenuPageObject.getMenuItem('Third'), [Keys.TAB]);
+    await MenuPageObject.sendKeys(MenuPageObject.getMenuItem('First'), [Keys.TAB]);
     expect(
-      await MenuPageObject.compareAttribute(MenuPageObject.getMenuItem('Fourth'), Attribute.IsFocused, AttributeValue.true),
+      await MenuPageObject.compareAttribute(MenuPageObject.getMenuItem('Third'), Attribute.IsFocused, AttributeValue.true),
     ).toBeTruthy();
 
-    await MenuPageObject.sendKeys(MenuPageObject.getMenuItem('Fourth'), [Keys.TAB]);
+    await MenuPageObject.sendKeys(MenuPageObject.getMenuItem('Third'), [Keys.TAB]);
     expect(
       await MenuPageObject.compareAttribute(MenuPageObject.getMenuItem('First'), Attribute.IsFocused, AttributeValue.true),
     ).toBeTruthy();
diff --git a/apps/fluent-tester/src/TestComponents/Menu/E2EMenuTest.tsx b/apps/fluent-tester/src/TestComponents/Menu/E2EMenuTest.tsx
index 6cd87ca1a0..6f7794709e 100644
--- a/apps/fluent-tester/src/TestComponents/Menu/E2EMenuTest.tsx
+++ b/apps/fluent-tester/src/TestComponents/Menu/E2EMenuTest.tsx
@@ -2,7 +2,7 @@ import * as React from 'react';
 import { View } from 'react-native';
 
 import { ButtonV1 as Button } from '@fluentui-react-native/button';
-import { Menu, MenuItem, MenuList, MenuPopover, MenuTrigger } from '@fluentui-react-native/menu';
+import { Menu, MenuDivider, MenuGroup, MenuItem, MenuList, MenuPopover, MenuTrigger } from '@fluentui-react-native/menu';
 import { Stack } from '@fluentui-react-native/stack';
 import { TextV1 as Text } from '@fluentui-react-native/text';
 
@@ -76,36 +76,41 @@ export const E2EMenuTest: React.FunctionComponent = () => {
             {...testProps(MENUPOPOVER_TEST_COMPONENT)}
           >
             <MenuList>
-              <MenuItem
-                onClick={onItemClick}
-                accessibilityLabel={MENUITEM_ACCESSIBILITY_LABEL}
-                persistOnClick
-                /* For Android E2E testing purposes, testProps must be passed in after accessibilityLabel. */
-                {...testProps(MENUITEM_TEST_COMPONENT)}
-              >
-                A plain MenuItem
-              </MenuItem>
-              <MenuItem
-                onClick={onItemClick}
-                /* For Android E2E testing purposes, testProps must be passed in after accessibilityLabel. */
-                {...testProps(MENUITEM_DISABLED_COMPONENT)}
-                disabled
-                persistOnClick
-              >
-                A second disabled plain MenuItem
-              </MenuItem>
-              <MenuItem
-                /* For Android E2E testing purposes, testProps must be passed in after accessibilityLabel. */
-                {...testProps(MENUITEM_NO_A11Y_LABEL_COMPONENT)}
-              >
-                {MENUITEM_TEST_LABEL}
-              </MenuItem>
-              <MenuItem
-                /* For Android E2E testing purposes, testProps must be passed in after accessibilityLabel. */
-                {...testProps(MENUITEM_FOURTH_COMPONENT)}
-              >
-                A fourth plain MenuItem
-              </MenuItem>
+              <MenuGroup>
+                <MenuItem
+                  onClick={onItemClick}
+                  accessibilityLabel={MENUITEM_ACCESSIBILITY_LABEL}
+                  persistOnClick
+                  /* For Android E2E testing purposes, testProps must be passed in after accessibilityLabel. */
+                  {...testProps(MENUITEM_TEST_COMPONENT)}
+                >
+                  A plain MenuItem
+                </MenuItem>
+                <MenuItem
+                  onClick={onItemClick}
+                  /* For Android E2E testing purposes, testProps must be passed in after accessibilityLabel. */
+                  {...testProps(MENUITEM_DISABLED_COMPONENT)}
+                  disabled
+                  persistOnClick
+                >
+                  A second disabled plain MenuItem
+                </MenuItem>
+              </MenuGroup>
+              <MenuDivider />
+              <MenuGroup>
+                <MenuItem
+                  /* For Android E2E testing purposes, testProps must be passed in after accessibilityLabel. */
+                  {...testProps(MENUITEM_NO_A11Y_LABEL_COMPONENT)}
+                >
+                  {MENUITEM_TEST_LABEL}
+                </MenuItem>
+                <MenuItem
+                  /* For Android E2E testing purposes, testProps must be passed in after accessibilityLabel. */
+                  {...testProps(MENUITEM_FOURTH_COMPONENT)}
+                >
+                  A fourth plain MenuItem
+                </MenuItem>
+              </MenuGroup>
             </MenuList>
           </MenuPopover>
         </Menu>
diff --git a/change/@fluentui-react-native-e2e-testing-32828e64-4b64-492f-81be-792e3251f3dc.json b/change/@fluentui-react-native-e2e-testing-32828e64-4b64-492f-81be-792e3251f3dc.json
new file mode 100644
index 0000000000..22993229a8
--- /dev/null
+++ b/change/@fluentui-react-native-e2e-testing-32828e64-4b64-492f-81be-792e3251f3dc.json
@@ -0,0 +1,7 @@
+{
+  "type": "patch",
+  "comment": "update E2E test for win32",
+  "packageName": "@fluentui-react-native/e2e-testing",
+  "email": "krsiler@microsoft.com",
+  "dependentChangeType": "patch"
+}
diff --git a/change/@fluentui-react-native-menu-b17320f8-20c5-4a54-970d-631cdc6f9288.json b/change/@fluentui-react-native-menu-b17320f8-20c5-4a54-970d-631cdc6f9288.json
new file mode 100644
index 0000000000..8e6f406bde
--- /dev/null
+++ b/change/@fluentui-react-native-menu-b17320f8-20c5-4a54-970d-631cdc6f9288.json
@@ -0,0 +1,7 @@
+{
+  "type": "patch",
+  "comment": "Fix Menu tab navigation on win32",
+  "packageName": "@fluentui-react-native/menu",
+  "email": "krsiler@microsoft.com",
+  "dependentChangeType": "patch"
+}
diff --git a/change/@fluentui-react-native-tester-98ce1cf5-f9a2-4543-8967-482d23186b0e.json b/change/@fluentui-react-native-tester-98ce1cf5-f9a2-4543-8967-482d23186b0e.json
new file mode 100644
index 0000000000..365fbbb5a5
--- /dev/null
+++ b/change/@fluentui-react-native-tester-98ce1cf5-f9a2-4543-8967-482d23186b0e.json
@@ -0,0 +1,7 @@
+{
+  "type": "patch",
+  "comment": "update E2E test for win32",
+  "packageName": "@fluentui-react-native/tester",
+  "email": "krsiler@microsoft.com",
+  "dependentChangeType": "patch"
+}
diff --git a/packages/components/Menu/src/MenuList/MenuList.tsx b/packages/components/Menu/src/MenuList/MenuList.tsx
index 2e256b4339..b3f66ced51 100644
--- a/packages/components/Menu/src/MenuList/MenuList.tsx
+++ b/packages/components/Menu/src/MenuList/MenuList.tsx
@@ -37,7 +37,6 @@ MenuStack.displayName = 'MenuStack';
 const shouldHaveFocusZone = ['macos', 'win32'].includes(Platform.OS as string);
 const focusLandsOnContainer = Platform.OS === 'macos';
 const hasCircularNavigation = Platform.OS === ('win32' as any);
-const hasTabNavigation = Platform.OS === ('win32' as any);
 
 export const menuListLookup = (layer: string, state: MenuListState, userProps: MenuListProps): boolean => {
   return state[layer] || userProps[layer] || layer === 'hasMaxHeight';
@@ -84,6 +83,13 @@ export const MenuList = compose<MenuListType>({
       const shouldHaveScrollView = Platform.OS === 'macos' || menuList.hasMaxHeight || menuList.hasMaxWidth;
       const ScrollViewWrapper = shouldHaveScrollView ? Slots.scrollView : React.Fragment;
 
+      const hasMenuGroupChild = React.Children.toArray(children).some(
+        (child) => child && (child as any).type && (child as any).type.displayName === 'MenuGroup',
+      );
+
+      // On win32, tab navigation should only be enabled when we have menu groups.
+      const hasTabNavigation = Platform.OS === ('win32' as any) && hasMenuGroupChild;
+
       const content = (
         <Slots.root>
           <ScrollViewWrapper