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Add travis badge. Links from develop to master
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README.md

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[![Build Status](https://travis-ci.org/mikessh/migec.svg?branch=master)](https://travis-ci.org/mikessh/migmap.svg?branch=master)
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# MiGMAP: mapper for full-length T- and B-cell repertoire sequencing
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In a nutshell, this software is a smart wrapper for [IgBlast](http://www.ncbi.nlm.nih.gov/igblast/igblast.cgi) V-(D)-J mapping tool designed to facilitate analysis immune receptor libraries profiled using high-throughput sequencing.
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- ``out.shm.txt`` a flat file with all mutations present in sample that can be processed with ``post/analyze_shm.Rmd`` [R markdown](http://rmarkdown.rstudio.com/) template.
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- ``out.net.txt``, ``out.node.txt`` and ``out.edge.txt`` (network, node and edge properties) that can be imported to [Cytoscape](http://www.cytoscape.org/) using ``Import>Table>`` menu.
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For more details and an example analysis of hypermutating Raji cell repertoire go to ``post/`` folder in this repository (or click [here](https://github.com/mikessh/migmap/tree/develop/post)).
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For more details and an example analysis of hypermutating Raji cell repertoire go to ``post/`` folder in this repository (or click [here](https://github.com/mikessh/migmap/tree/master/post)).
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## Output format

post/README.md

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### Analysis of somatic hypermutation profile
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![Rmd SHM analysis](https://github.com/mikessh/migmap/blob/develop/post/analyze_shm.png)
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![Rmd SHM analysis](https://github.com/mikessh/migmap/blob/master/post/analyze_shm.png)
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- ``raji_R12.shm.txt`` - mutation table
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- ``analyze_shm.Rmd`` - analysis template
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The whole analysis template is stored in R markdown format, which means it can be loaded to [Rstudio](http://rmarkdown.rstudio.com/), customized and executed. Detailed explanation of all analysis steps and plots is embedded into the template. An example PDF output is shown in ``analyze_shm.pdf`` (click [here](https://github.com/mikessh/migmap/blob/develop/post/analyze_shm.pdf) to view it).
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The whole analysis template is stored in R markdown format, which means it can be loaded to [Rstudio](http://rmarkdown.rstudio.com/), customized and executed. Detailed explanation of all analysis steps and plots is embedded into the template. An example PDF output is shown in ``analyze_shm.pdf`` (click [here](https://github.com/mikessh/migmap/blob/master/post/analyze_shm.pdf) to view it).
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### Clonotype tree
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![Rmd SHM analysis](https://github.com/mikessh/migmap/blob/develop/post/raji.png)
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![Rmd SHM analysis](https://github.com/mikessh/migmap/blob/master/post/raji.png)
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### Clonotype network
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**Under development** The concept is a network showing each CDR3 with its children (edges are weighted by hypermutation load/S:R ratio) and edges between distinct CDR3 which are likely hypermutations. E.g. a single mutation within "N" region of CDR3 and consequent match of 5 "N" nucleotides between two different CDR3 tells that they are more likely a result of hypermutation than of independent V-D-J recombination.
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**Under masterment** The concept is a network showing each CDR3 with its children (edges are weighted by hypermutation load/S:R ratio) and edges between distinct CDR3 which are likely hypermutations. E.g. a single mutation within "N" region of CDR3 and consequent match of 5 "N" nucleotides between two different CDR3 tells that they are more likely a result of hypermutation than of independent V-D-J recombination.

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