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CHANGELOG.md

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The format is based on [Keep a Changelog](http://keepachangelog.com/)
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and this project adheres to [Semantic Versioning](http://semver.org/).
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## [WIP]
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## [v2.4]
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### Changes
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- Publish the "KrakenUniq parsed report" in the 'stats' directory
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- Add an R-friendly version of the final summary report (column names w/o special characters)
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- Add an R-friendly version of the final summary report (column names w/o special characters, `R_final_report.tsv`)
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- In the final_report, replace `SpeciesKmers` name with `Kmers` and include the "best" and "(family)" level stats. E.g. "4 (129)"
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- for the `KmerCoverage` and `KmerDupRate` columns, also combine "best" and "(family)"
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- Add a `--fixed_bedfiltering` flag to run dustmasking and bedfiltering also for fixed references (off by default)

README.md

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# set this if you encounter a heap-space error to increase the memory that is used by nextflow
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export NXF_OPTS="-Xms10g -Xmx15g" # increase or decrease the numbers as required
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nextflow run mpieva/quicksand -r v2.3 \
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nextflow run mpieva/quicksand -r v2.4 \
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--db refseq/kraken/Mito_db_kmer22/ \
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--genomes refseq/genomes/ \
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--bedfiles refseq/masked/ \

assets/pipeline/help.txt

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Usage: nextflow run mpieva/quicksand -r v2.3 {--bam PATH --rg PATH | --split DIR} --db PATH --genome PATH --bedfiles PATH [options] -profile singularity
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Usage: nextflow run mpieva/quicksand -r v2.4 {--bam PATH --rg PATH | --split DIR} --db PATH --genome PATH --bedfiles PATH [options] -profile singularity
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required arguments:
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Either:

docs/source/conf.py

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author = 'Merlin Szymanski'
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# The full version, including alpha/beta/rc tags
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release = 'v2.3'
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version = 'v2.3'
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release = 'v2.4'
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version = 'v2.4'
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# -- General configuration ---------------------------------------------------
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docs/source/examples.rst

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Execute quicksand like this::
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nextflow run mpieva/quicksand -r v2.3 \
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nextflow run mpieva/quicksand -r v2.4 \
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-profile singularity \
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--split split/ \
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--db refseq/kraken/Mito_db_kmer22/ \
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(after KrakenUniq) are stored in :code:`out/{family}/1-extracted/` while mapped, deduped and bedfiltered sequences are saved in the
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:code:`out/{family}/best/{step}/` directories after the respective processing step::
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quicksand_v2.3
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quicksand_v2.4
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├── out
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│ └── {family}
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│ ├── 1-extracted
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Run quicksand with::
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nextflow run mpieva/quicksand -r v2.3 \
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nextflow run mpieva/quicksand -r v2.4 \
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-profile singularity \
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--split split/ \
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--db refseq/kraken/Mito_db_kmer22/ \
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appear in the :code:`out/{family}/fixed/{step}/` directory, together with additional output-files
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that might be useful for additional downstream-analyses, such as the extracted deaminated reads::
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quicksand_v2.3
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quicksand_v2.4
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├── out
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│ └── {family}
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│ ├── 1-extracted
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and rerun the pipeline with::
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nextflow run mpieva/quicksand -r v2.3 \
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nextflow run mpieva/quicksand -r v2.4 \
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-profile singularity \
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--rerun \
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--fixed fixed-references.tsv
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The (additional) output files are then the ones created by the :code:`--fixed` flag::
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quicksand_v2.3
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quicksand_v2.4
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├── out
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│ └── Suidae
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│ ├── 1-extracted

docs/source/in_and_out.rst

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Output
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------
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quicksand writes all output files to the **quicksand_v2.3** directory. Within this directory the files are
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quicksand writes all output files to the **quicksand_v2.4** directory. Within this directory the files are
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layed out as follows::
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quicksand_v2.3
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quicksand_v2.4
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├── out
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│ └── {taxon}
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│ ├── 1-extracted

docs/source/quickstart.rst

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quicksand is executed directly from github. With the databases created and the testdata downloaded, run the pipeline as follows::
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nextflow run mpieva/quicksand -r v2.3 \
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nextflow run mpieva/quicksand -r v2.4 \
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-profile singularity \
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--db refseq/kraken/Mito_db_kmer22 \
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--genomes refseq/genomes/ \
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--bedfiles refseq/masked/ \
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--split split/
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The output of quicksand can be found in the directory **quicksand_v2.3/**
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The output of quicksand can be found in the directory **quicksand_v2.4/**
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See the :code:`final_report.tsv` and :code:`filtered_report_0.5p_0.5b.tsv` for a summary of the results.
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nextflow.config

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homePage = 'https://mpieva.github.io/quicksand/'
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description = 'quick analysis of sedimentary ancient DNA'
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nextflowVersion = '>=22.10'
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version = 'v2.3'
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version = 'v2.4'
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}
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cleanup = true

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