@@ -38,12 +38,12 @@ You just need to provide the two fusion gene partners (gene symbol, Ensembl ID,
3838Example usage from the command line:
3939
4040```
41- agfusion annotate \
42- --gene5prime DLG1 \
43- --gene3prime BRAF \
44- --junction5prime 31684294 \
45- --junction3prime 39648486 \
46- -db agfusion.mus_musculus.87.db \
41+ agfusion annotate
42+ --gene5prime DLG1
43+ --gene3prime BRAF
44+ --junction5prime 31684294
45+ --junction3prime 39648486
46+ -db agfusion.mus_musculus.87.db
4747 -o DLG1-BRAF
4848```
4949
@@ -61,13 +61,13 @@ You can additionally plot the wild-type proteins and exon structures for each ge
6161
6262```
6363agfusion annotate \
64- -g5 ENSMUSG00000022770 \
65- -g3 ENSMUSG00000002413 \
66- -j5 31684294 \
67- -j3 39648486 \
68- -db agfusion.mus_musculus.87.db \
69- -o DLG1-BRAF \
70- --WT
64+ -g5 ENSMUSG00000022770 \
65+ -g3 ENSMUSG00000002413 \
66+ -j5 31684294 \
67+ -j3 39648486 \
68+ -db agfusion.mus_musculus.87.db \
69+ -o DLG1-BRAF \
70+ --WT
7171```
7272
7373### Canonical gene isoforms
@@ -168,13 +168,13 @@ First you need to install pyensembl (and the other dependencies listed at the bo
168168
169169```
170170For GRCh38/hg38:
171- pyensembl install --species homo_sapiens --release 87
171+ pyensembl install --species homo_sapiens --release 87
172172
173173For GRCh37/hg19:
174- pyensembl install --species homo_sapiens --release 75
174+ pyensembl install --species homo_sapiens --release 75
175175
176176For GRCm38/mm10:
177- pyensembl install --species mus_musculus --release 87
177+ pyensembl install --species mus_musculus --release 87
178178```
179179
180180Then you can install AGFusion:
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