Upgrading 21.10.6 to 23.10.1 resulting in command not found

[Subject-13]

Hello,

I recently tried out a new anaconda environment with nextflow=23.10.1. Also, I installed the same packages (also the same version) within the new environment. My nextflow call implies a variable --env env.yaml with all the required packages for my main.nf. When I run the main.nf script, nextflow finds all selfmade scripts under bin/, but can't find an installed tool (command not found). Nextflow creates the environment of my env.yaml, also installs the command-line tool it can't find. But it fails at this process. The process looks like:

process foo {
    input:
    something
    
    output:
    something

    script:
    """
    seqkit fq2fa ${file} -o ${file.simpleName}.fasta
    seqkit seq ${file.simpleName}.fasta -m 100 -g > ${reads.simpleName}_limit_100.fasta
    """
}

The error says:

ERROR ~ Error executing process > 'complete:ABC (null)'

Caused by:
  Process `complete:ABC (null)` terminated with an error exit status (127)

Command executed:

  seqkit fq2fa fo.fastq -o fo.fasta
  seqkit seq foo.fasta -m 100 -g > foo_limit_100.fasta

Command exit status:
  127

Command output:
  (empty)

Command error:
  .command.sh: line 2: seqkit: command not found

Work dir:
  .../nextflow/work/52/453d9c220f97bc9c1796532d0ebfb4

BUT. When I run the same nextflow call with NXF_VER=21.10.6 in front, within the same anaconda environment where I installed nextflow=23.10.1, the script runs without an error. That's why I'm wondering if it has something to do with the version. Do I oversee something or are there any big changes between those two used versions of nextflow?

Thanks a lot in advance!

EDIT: In nextflow.config I define a profile with process.conda = "${params.env}" for the env.yaml I pass through.

[Subject-13]

Digging through the Conda environments doc solved the problem. Since nextflow=22.08.0-edge it requires to set conda.enabled = true in your nextflow.config.