99 * defined by the Mozilla Public License, v. 2.0.
1010 *
1111 */
12-
12+
1313manifest {
14- description = ' Proof of concept of a RNA-seq pipeline implemented with Nextflow'
14+ description = ' Proof of concept of a RNA-seq pipeline implemented with Nextflow'
1515 author = ' Paolo Di Tommaso'
1616 nextflowVersion = ' >=23.10.0'
1717}
1818
19- /*
20- * default params
19+ /*
20+ * default params
2121 */
22-
22+
2323params. outdir = " results"
2424params. reads = " ${ projectDir} /data/ggal/ggal_gut_{1,2}.fq"
2525params. transcriptome = " ${ projectDir} /data/ggal/ggal_1_48850000_49020000.Ggal71.500bpflank.fa"
2626params. multiqc = " ${ projectDir} /multiqc"
2727
28- /*
29- * defines execution profiles for different environments
30- */
28+ /*
29+ * defines execution profiles for different environments
30+ */
3131
3232profiles {
3333 standard {
@@ -66,15 +66,21 @@ profiles {
6666
6767 ' conda' {
6868 conda. enabled = true
69- conda. channels = ' seqera, conda-forge,bioconda,defaults'
69+ conda. channels = ' conda-forge,bioconda,defaults'
7070 }
71-
71+
72+ ' mamba' {
73+ conda. enabled = true
74+ conda. useMicromamba = true
75+ conda. channels = ' conda-forge,bioconda,defaults'
76+ }
77+
7278 ' slurm' {
7379 process. container = ' docker.io/nextflow/rnaseq-nf:v1.3.0'
7480 process. executor = ' slurm'
7581 singularity. enabled = true
7682 }
77-
83+
7884 ' batch' {
7985 params. reads = ' s3://rnaseq-nf/data/ggal/lung_{1,2}.fq'
8086 params. transcriptome = ' s3://rnaseq-nf/data/ggal/transcript.fa'
@@ -90,7 +96,7 @@ profiles {
9096 process. container = ' docker.io/nextflow/rnaseq-nf:v1.3.0'
9197 params. reads = ' s3://rnaseq-nf/data/ggal/lung_{1,2}.fq'
9298 params. transcriptome = ' s3://rnaseq-nf/data/ggal/transcript.fa'
93- }
99+ }
94100
95101 ' google-batch' {
96102 params. transcriptome = ' gs://rnaseq-nf/data/ggal/transcript.fa'
@@ -117,7 +123,7 @@ profiles {
117123 azure {
118124 batch {
119125 location = ' westeurope'
120- accountName = " $AZURE_BATCH_ACCOUNT_NAME "
126+ accountName = " $AZURE_BATCH_ACCOUNT_NAME "
121127 accountKey = " $AZURE_BATCH_ACCOUNT_KEY "
122128 autoPoolMode = true
123129 deletePoolsOnCompletion = true
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