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Bump MultiQC container in NF4 RNAseq (#687)
* Bump MQC container * Fix leftover header numbering --------- Co-authored-by: Geraldine Van der Auwera <[email protected]>
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docs/nf4_science/rnaseq/01_method.md

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@@ -157,7 +157,7 @@ ENCSR000COQ1_1.fastq.gz_trimming_report.txt ENCSR000COQ1_1_trimmed_fastqc.html
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ENCSR000COQ1_1_trimmed.fq.gz ENCSR000COQ1_1_trimmed_fastqc.zip
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```
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### 1.6. Move the output files to the filesystem outside the container
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### 1.5. Move the output files to the filesystem outside the container
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Anything that remains inside the container will be inaccessible to future work so let's move these to a new directory.
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@@ -166,7 +166,7 @@ mkdir /data/trimmed
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mv ENCSR000COQ1_1* /data/trimmed
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```
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### 1.7. Exit the container
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### 1.6. Exit the container
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```bash
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exit
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This stores a `genome_index.tar.gz` tarball containing the genome index files in the `data/` directory on our filesystem, which wil come in handy in Part 2 of this course.
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### 2.5. Run the `hisat2` command
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### 2.4. Run the `hisat2` command
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Now we can run the alignment command, which performs the alignment step with `hisat2` then pipes the output to `samtools` to write the output out as a BAM file.
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ENCSR000COQ1_1_trimmed.bam ENCSR000COQ1_1_trimmed.hisat2.log
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```
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### 2.6. Move the output files to the filesystem outside the container
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### 2.5. Move the output files to the filesystem outside the container
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```bash
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mkdir /data/aligned
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mv ENCSR000COQ1_1* /data/aligned
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```
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### 2.7. Exit the container
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### 2.6. Exit the container
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```bash
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exit
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### 3.1. Pull the `multiqc` container
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```bash
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docker pull community.wave.seqera.io/library/pip_multiqc:ad8f247edb55897c
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docker pull community.wave.seqera.io/library/pip_multiqc:a3c26f6199d64b7c
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```
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```console title="Output"
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3f229294c69a: Pull complete
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5a5ad47fd84c: Pull complete
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Digest: sha256:0ebb1d9605395a7df49ad0eb366b21f46afd96a5090376b0d8941cf5294a895a
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Status: Downloaded newer image for community.wave.seqera.io/library/pip_multiqc:ad8f247edb55897c
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community.wave.seqera.io/library/pip_multiqc:ad8f247edb55897c
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Status: Downloaded newer image for community.wave.seqera.io/library/pip_multiqc:a3c26f6199d64b7c
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community.wave.seqera.io/library/pip_multiqc:a3c26f6199d64b7c
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```
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### 3.2. Spin up the `multiqc` container interactively
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```bash
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docker run -it -v ./data:/data community.wave.seqera.io/library/pip_multiqc:ad8f247edb55897c
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docker run -it -v ./data:/data community.wave.seqera.io/library/pip_multiqc:a3c26f6199d64b7c
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```
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### 3.3. Run the `multiqc` command

docs/nf4_science/rnaseq/03_multi-sample.md

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process MULTIQC {
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container "community.wave.seqera.io/library/pip_multiqc:ad8f247edb55897c"
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container "community.wave.seqera.io/library/pip_multiqc:a3c26f6199d64b7c"
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publishDir "results/multiqc", mode: 'symlink'
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input:

nf4-science/rnaseq/solutions/modules/multiqc.nf

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process MULTIQC {
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container "community.wave.seqera.io/library/pip_multiqc:ad8f247edb55897c"
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container "community.wave.seqera.io/library/pip_multiqc:a3c26f6199d64b7c"
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publishDir "results/multiqc", mode: 'symlink'
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input:

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