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@@ -6,7 +6,9 @@ We try to manage the required tasks for nf-core/chipseq using GitHub issues, you
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However, don't be put off by this template - other more general issues and suggestions are welcome! Contributions to the code are even more welcome ;)
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> If you need help using or modifying nf-core/chipseq then the best place to ask is the nf-core `chipseq` channel on [Slack](https://nf-core-invite.herokuapp.com/).
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> If you need help using or modifying nf-core/chipseq then the best place to ask is on the pipeline channel on [Slack](https://nf-core-invite.herokuapp.com/).
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## Contribution workflow
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If you'd like to write some code for nf-core/chipseq, the standard workflow
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These tests are run both with the latest available version of Nextflow and also the minimum required version that is stated in the pipeline code.
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## Getting help
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For further information/help, please consult the [nf-core/chipseq documentation](https://github.com/nf-core/chipseq#documentation) and don't hesitate to get in touch on the nf-core `chipseq` channel on [Slack](https://nf-core-invite.herokuapp.com/).
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For further information/help, please consult the [nf-core/chipseq documentation](https://github.com/nf-core/chipseq#documentation) and don't hesitate to get in touch on the pipeline channel on [Slack](https://nf-core-invite.herokuapp.com/).
Nextflow handles job submissions on SLURM or other environments, and supervises running the jobs. Thus the Nextflow process must run until the pipeline is finished. We recommend that you put the process running in the background through `screen` / `tmux` or similar tool. Alternatively you can run nextflow within a cluster job submitted your job scheduler.
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This version number will be logged in reports when you run the pipeline, so that you'll know what you used when you look back in the future.
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## Main arguments
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### `-profile`
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If `-profile` is not specified at all the pipeline will be run locally and expects all software to be installed and available on the `PATH`.
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*`awsbatch`
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* A generic configuration profile to be used with AWS Batch
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* A generic configuration profile to be used with AWS Batch.
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*`conda`
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* A generic configuration profile to be used with [conda](https://conda.io/docs/)
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* Pulls most software from [Bioconda](https://bioconda.github.io/)
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* Pulls software from dockerhub: [`nfcore/chipseq`](http://hub.docker.com/r/nfcore/chipseq/)
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*`singularity`
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* A generic configuration profile to be used with [Singularity](http://singularity.lbl.gov/)
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* Pulls software from dockerhub: [`nfcore/chipseq`](http://hub.docker.com/r/nfcore/chipseq/)
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* Pulls software from DockerHub: [`nfcore/chipseq`](http://hub.docker.com/r/nfcore/chipseq/)
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*`test`
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* A profile with a complete configuration for automated testing
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* Includes links to test data so needs no other parameters
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### `--genome` (using iGenomes)
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There are 31 different species supported in the iGenomes references. To run the pipeline, you must specify which to use with the `--genome` flag.
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You can find the keys to specify the genomes in the [`iGenomes config file`](../conf/igenomes.config). Common genomes that are supported are:
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You can find the keys to specify the genomes in the [iGenomes config file](../conf/igenomes.config). Common genomes that are supported are:
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* Human
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*`--genome GRCh37`
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*_Drosophila_
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*`--genome BDGP6`
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*_S. cerevisiae_
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*`--genome R64-1-1`
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*`--genome 'R64-1-1'`
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> There are numerous others - check the config file for more.
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Specify the path to a specific config file (this is a core NextFlow command).
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**NB:** Single hyphen (core Nextflow option)
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**NB:** You can use this config to override pipeline defaults.
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Note - you can use this to override pipeline defaults.
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### `--custom_config_version`
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Provide git commit id for custom Institutional configs hosted at `nf-core/configs`. This was implemented for reproducibility purposes. Default is set to `master`.
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## Run the pipeline
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cd /path/to/my/data
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nextflow run /path/to/chipseq_pipeline/ --custom_config_base /path/to/my/configs/configs-master/
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nextflow run /path/to/pipeline/ --custom_config_base /path/to/my/configs/configs-master/
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```
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> Note that the nf-core/tools helper package has a `download` command to download all required pipeline
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