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Update docs/output.md
Co-authored-by: Jose Espinosa-Carrasco <[email protected]>
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docs/output.md

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@@ -261,7 +261,7 @@ The [featureCounts](http://bioinf.wehi.edu.au/featureCounts/) tool is used to co
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**This pipeline uses a standardised DESeq2 analysis script to get an idea of the reproducibility within the experiment, and to assess the overall differential binding. Please note that this will not suit every experimental design, and if there are other problems with the experiment then it may not work as well as expected.**
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For larger experiments, it is recommended to use the `vst` transformation instead of the default `rlog` option. This is the default behaviour and can be controlled with the `--deseq2_vst` parameter. See [DESeq2 docs](http://bioconductor.org/packages/devel/bioc/vignettes/DESeq2/inst/doc/DESeq2.html#data-transformations-and-visualization) for a more detailed explanation.
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For larger experiments, it is recommended to use the `vst` transformation instead of the `rlog` option. This is the default behaviour and can be controlled with the `--deseq2_vst` parameter. See [DESeq2 docs](http://bioconductor.org/packages/devel/bioc/vignettes/DESeq2/inst/doc/DESeq2.html#data-transformations-and-visualization) for a more detailed explanation.
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![MultiQC - DESeq2 PCA plot](images/mqc_deseq2_pca_plot.png)
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