diff --git a/modules/nf-core/samtools/samples/environment.yml b/modules/nf-core/samtools/samples/environment.yml
new file mode 100644
index 00000000000..bac7feb287b
--- /dev/null
+++ b/modules/nf-core/samtools/samples/environment.yml
@@ -0,0 +1,7 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - "bioconda::samtools=1.22.1"
diff --git a/modules/nf-core/samtools/samples/main.nf b/modules/nf-core/samtools/samples/main.nf
new file mode 100644
index 00000000000..0dc190cbd1b
--- /dev/null
+++ b/modules/nf-core/samtools/samples/main.nf
@@ -0,0 +1,66 @@
+process SAMTOOLS_SAMPLES {
+    tag "$meta.id"
+    label 'process_single'
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/samtools:1.22.1--h96c455f_0':
+        'biocontainers/samtools:1.22.1--h96c455f_0' }"
+
+    input:
+    tuple val(meta) , path(bam)  , path(bai)
+    tuple val(meta2), path(fasta), path(fai)
+
+    output:
+    tuple val(meta), path("*.tsv"), emit: tsv
+    path "versions.yml"           , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    // Wrapping fasta in a list in case there is exactly one (in that case it's a bare path)
+    def fasta_arg = fasta ? [fasta].flatten().collect { "-f $it" }.join(' ') : ''
+    def out_arg = "-o ${prefix}.tsv"
+    def bai_arg = args.contains('-X') ? bai : ''
+    """
+    samtools samples \\
+        $args \\
+        $fasta_arg \\
+        $out_arg \\
+        $bam \\
+        $bai_arg
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
+    END_VERSIONS
+    """
+
+    stub:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    // Write header if requested because the output will likely be parsed by Nextflow
+    def headers = ''
+    if (args.contains('-h\\n')) {
+        headers = "#SM\tPATH"
+        if (args.contains('-i')) {
+            headers += "\tINDEX"
+        }
+        if (fasta) {
+            headers += "\tREFERENCE"
+        }
+    }
+    """
+    echo $args
+
+    echo -ne "$headers" > ${prefix}.tsv
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/samtools/samples/meta.yml b/modules/nf-core/samtools/samples/meta.yml
new file mode 100644
index 00000000000..ff04e802a04
--- /dev/null
+++ b/modules/nf-core/samtools/samples/meta.yml
@@ -0,0 +1,71 @@
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json
+name: "samtools_samples"
+description: |
+  Write sample names and path to reference genome of an alignment to a text file.
+keywords:
+  - samples
+  - bam
+  - cram
+  - genomics
+tools:
+  - samtools:
+      description: |
+        SAMtools is a set of utilities for interacting with and post-processing
+        short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li.
+        These files are generated as output by short read aligners like BWA.
+      homepage: http://www.htslib.org/
+      documentation: http://www.htslib.org/doc/samtools.html
+      doi: 10.1093/bioinformatics/btp352
+      licence: ["MIT"]
+      identifier: biotools:samtools
+
+input:
+  - - meta:
+        type: map
+        description: Groovy Map containing sample information. e.g. `[ id:'sample1'
+          ]`
+    - bam:
+        type: file
+        description: alignment file
+        pattern: "*.{bam,sam,cram}"
+        ontologies:
+          - edam: http://edamontology.org/format_2572 # BAM
+          - edam: http://edamontology.org/format_2573 # SAM
+          - edam: http://edamontology.org/format_3462 # CRAM
+    - bai:
+        type: file
+        description: index file for the alignment
+        pattern: "*.{bai,crai}"
+  - - meta2:
+        type: map
+        description: Groovy Map containing reference information.
+    - fasta:
+        type: file
+        description: reference genome file (optional)
+        pattern: "*.{fasta,fa,fna}"
+        ontologies:
+          - edam: http://edamontology.org/format_1929 # FASTA
+    - fai:
+        type: file
+        description: index file for the reference genome (optional)
+        pattern: "*.fai"
+output:
+  tsv:
+    - - meta:
+          type: map
+          description: Groovy Map containing sample information. e.g. `[ id:'sample1' ]`
+      - "*.tsv":
+          type: file
+          description: tab-separated file containing sample names, BAM filename and optionally reference genome path
+          pattern: "*.tsv"
+  versions:
+    - versions.yml:
+        type: file
+        description: File containing software versions
+        pattern: versions.yml
+        ontologies:
+          - edam: http://edamontology.org/format_3750 # YAML
+authors:
+  - "@Schmytzi"
+maintainers:
+  - "@Schmytzi"
diff --git a/modules/nf-core/samtools/samples/tests/main.nf.test b/modules/nf-core/samtools/samples/tests/main.nf.test
new file mode 100644
index 00000000000..2769e43b02c
--- /dev/null
+++ b/modules/nf-core/samtools/samples/tests/main.nf.test
@@ -0,0 +1,115 @@
+nextflow_process {
+
+    name "Test Process SAMTOOLS_SAMPLES"
+    script "../main.nf"
+    process "SAMTOOLS_SAMPLES"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "samtools"
+    tag "samtools/samples"
+
+    test("sarscov2 - [bam, bai], []") {
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:false ],
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true),
+                ]
+                input[1] = [ [], [], [] ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+    test("sarscov2 - [bam, bai], [fasta, fai]") {
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:false ],
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true)
+                ]
+                input[1] = [
+                  [ id : 'sarscov2' ],
+                  file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true),
+                  file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+
+
+    test("sarscov2 - [bam, bai], [] - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:false ],
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true),
+                ]
+                input[1] = [ [], [], [] ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("sarscov2 - [bam, bai], [fasta, fai] - stub") {
+        options "-stub"
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:false ],
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true)
+                ]
+                input[1] = [
+                  [ id : 'sarscov2' ],
+                  file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true),
+                  file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }}
diff --git a/modules/nf-core/samtools/samples/tests/main.nf.test.snap b/modules/nf-core/samtools/samples/tests/main.nf.test.snap
new file mode 100644
index 00000000000..31009be0ae4
--- /dev/null
+++ b/modules/nf-core/samtools/samples/tests/main.nf.test.snap
@@ -0,0 +1,142 @@
+{
+    "sarscov2 - [bam, bai], [] - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,22787e093202243f0113dac1dec9586f"
+                ],
+                "tsv": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,22787e093202243f0113dac1dec9586f"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.5"
+        },
+        "timestamp": "2025-10-31T10:33:18.753345922"
+    },
+    "sarscov2 - [bam, bai], [fasta, fai]": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.tsv:md5,c0299b95ede533c8fdf8190b32edf3c1"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,22787e093202243f0113dac1dec9586f"
+                ],
+                "tsv": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.tsv:md5,c0299b95ede533c8fdf8190b32edf3c1"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,22787e093202243f0113dac1dec9586f"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.5"
+        },
+        "timestamp": "2025-10-31T10:38:41.413689014"
+    },
+    "sarscov2 - [bam, bai], []": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.tsv:md5,89b11a0b32cd311fb370feac23ed8b8a"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,22787e093202243f0113dac1dec9586f"
+                ],
+                "tsv": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.tsv:md5,89b11a0b32cd311fb370feac23ed8b8a"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,22787e093202243f0113dac1dec9586f"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.5"
+        },
+        "timestamp": "2025-10-31T10:35:35.165854514"
+    },
+    "sarscov2 - [bam, bai], [fasta, fai] - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,22787e093202243f0113dac1dec9586f"
+                ],
+                "tsv": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,22787e093202243f0113dac1dec9586f"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.5"
+        },
+        "timestamp": "2025-10-31T10:38:52.403523794"
+    }
+}
\ No newline at end of file