@@ -80,41 +80,41 @@ sample_names_replace_exact: true
8080
8181# Run only these modules
8282run_modules:
83- - custom_content
84- - fastqc
85- - cutadapt
86- - fastp
87- - sortmerna
88- - star
89- - hisat2
90- - rsem
91- - umitools
92- - salmon
93- - kallisto
94- - samtools
95- - picard
96- - preseq
97- - rseqc
98- - qualimap
99- - kraken
83+ - custom_content
84+ - fastqc
85+ - cutadapt
86+ - fastp
87+ - sortmerna
88+ - star
89+ - hisat2
90+ - rsem
91+ - umitools
92+ - salmon
93+ - kallisto
94+ - samtools
95+ - picard
96+ - preseq
97+ - rseqc
98+ - qualimap
99+ - kraken
100100
101101# Order of modules
102102
103103module_order:
104- - fastqc:
105- anchor: "fastqc_raw"
106- name: "FastQC (raw)"
107- info: "This section of the report shows FastQC results before adapter trimming."
108- path_filters:
109- - "*_raw*fastqc.zip"
110- - cutadapt
111- - fastp
112- - fastqc:
113- anchor: "fastqc_trimmed"
114- name: "FastQC (trimmed)"
115- info: "This section of the report shows FastQC results after adapter trimming."
116- path_filters:
117- - "*_trimmed*fastqc.zip"
104+ - fastqc:
105+ anchor: "fastqc_raw"
106+ name: "FastQC (raw)"
107+ info: "This section of the report shows FastQC results before adapter trimming."
108+ path_filters:
109+ - "*_raw*fastqc.zip"
110+ - cutadapt
111+ - fastp
112+ - fastqc:
113+ anchor: "fastqc_trimmed"
114+ name: "FastQC (trimmed)"
115+ info: "This section of the report shows FastQC results after adapter trimming."
116+ path_filters:
117+ - "*_trimmed*fastqc.zip"
118118
119119# Don't show % Dups in the General Stats table (we have this from Picard)
120120table_columns_visible:
@@ -124,10 +124,10 @@ table_columns_visible:
124124 percent_duplicates: false
125125
126126extra_fn_clean_exts:
127- - ".umi_dedup"
128- - "_val"
129- - ".markdup"
130- - "_primary"
127+ - ".umi_dedup"
128+ - "_val"
129+ - ".markdup"
130+ - "_primary"
131131
132132# Customise the module search patterns to speed up execution time
133133# - Skip module sub-tools that we are not interested in
160160 samtools/idxstats:
161161 fn: "*.idxstats*"
162162 kraken:
163- - fn: "*.kraken2.report.txt"
164- - fn: "*.kraken2.report_bracken.txt"
163+ - fn: "*.kraken2.report.txt"
164+ - fn: "*.kraken2.report_bracken.txt"
165165 rseqc/bam_stat:
166166 fn: "*.bam_stat.txt"
167167 rseqc/gene_body_coverage:
211211 skip: true
212212
213213fn_ignore_files:
214- - "*umi_dedup.transcriptome.sorted.log"
214+ - "*umi_dedup.transcriptome.sorted.log"
215215
216216# See https://github.com/ewels/MultiQC_TestData/blob/master/data/custom_content/with_config/table_headerconfig/multiqc_config.yaml
217217custom_data:
@@ -262,26 +262,26 @@ custom_data:
262262 description: User-provided strandedness
263263 cond_formatting_rules:
264264 blue:
265- - s_eq: "auto"
265+ - s_eq: "auto"
266266 black:
267- - s_eq: "forward"
267+ - s_eq: "forward"
268268 grey:
269- - s_eq: "reverse"
269+ - s_eq: "reverse"
270270 cond_formatting_colours:
271- - grey: "#AAB7B8"
272- - black: "#17202A"
271+ - grey: "#AAB7B8"
272+ - black: "#17202A"
273273 Inferred strandedness:
274274 description: Interred strandedness
275275 cond_formatting_rules:
276276 blue:
277- - s_eq: "auto"
277+ - s_eq: "auto"
278278 black:
279- - s_eq: "forward"
279+ - s_eq: "forward"
280280 grey:
281- - s_eq: "reverse"
281+ - s_eq: "reverse"
282282 cond_formatting_colours:
283- - grey: "#AAB7B8"
284- - black: "#17202A"
283+ - grey: "#AAB7B8"
284+ - black: "#17202A"
285285 Sense (%):
286286 max: 100
287287 Antisense (%):
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