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lines changed Original file line number Diff line number Diff line change @@ -402,13 +402,9 @@ class WorkflowRnaseq {
402402 " When using '--additional_fasta <FASTA_FILE>' the aligner index will not\n " +
403403 " be re-built with the transgenes incorporated by default since you have \n " +
404404 " already provided an index via '--${ index} _index <INDEX>'.\n\n " +
405- " Set '--additional_fasta <FASTA_FILE> --${ index} _index false --save_reference' to \n " +
406- " re-build the index with transgenes included and the index will be saved in\n " +
405+ " Set '--additional_fasta <FASTA_FILE> --${ index} _index false --gene_bed false -- save_reference'\n " +
406+ " to re-build the index with transgenes included and the index and gene BED file will be saved in\n " +
407407 " 'results/genome/index/${ index} /' for re-use with '--${ index} _index'.\n\n " +
408- " In addition, regions in '--additional_fasta' will not be included in count \n " +
409- " values. If you wish to include this supply a '--gene_bed' file including \n " +
410- " of interest or use '--gene_bed false to generate a new one containing all \n " +
411- " of the '--additional_fasta' regions.\n\n " +
412408 " Ignore this warning if you know that the index already contains transgenes.\n\n " +
413409 " Please see:\n " +
414410 " https://github.com/nf-core/rnaseq/issues/556\n " +
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