|
1 | 1 | process { |
2 | 2 |
|
| 3 | + // |
3 | 4 | // Apply software overrides for ARM compatibility |
4 | | - |
5 | | - withName: '.*:ALIGN_STAR:STAR_ALIGN_IGENOMES' { |
6 | | - conda = 'seqera::star=2.6.1d bioconda::samtools=1.21 conda-forge::gawk=5.1.0' |
7 | | - //conda = 'seqera::star=2.6.1d bioconda::samtools=1.10 conda-forge::gawk=5.1.0' |
8 | | - } |
9 | | - |
10 | | - withName: 'STAR_GENOMEGENERATE_IGENOMES' { |
11 | | - conda = 'bioconda::star bioconda::samtools=1.21 conda-forge::gawk=5.1.0' |
12 | | - //conda = 'seqera::star=2.6.1d bioconda::samtools=1.10 conda-forge::gawk=5.1.0' |
13 | | - } |
14 | | - |
15 | | - withName: 'BBSPLIT' { |
16 | | - conda = 'bioconda::bbmap' |
17 | | - // conda = 'bioconda::bbmap=39.01' |
18 | | - } |
19 | | - |
20 | | - withName: 'CAT_FASTQ' { |
21 | | - conda = 'conda-forge::coreutils' |
22 | | - //conda = 'conda-forge::coreutils=8.30' |
23 | | - } |
24 | | - |
25 | | - withName: 'CAT_ADDITIONAL_FASTA' { |
26 | | - conda = 'conda-forge::python' |
27 | | - //conda = 'conda-forge::coreutils=3.9.5' |
28 | | - } |
29 | | - |
| 5 | + // |
| 6 | + |
| 7 | + //// Tested, working, module updates in progress on main dev branch |
| 8 | + |
30 | 9 | withName: 'CUSTOM_GETCHROMSIZES' { |
31 | 10 | conda = 'bioconda::samtools=1.21' |
32 | 11 | //conda = 'bioconda::samtools=1.20 bioconda::htslib=1.20' |
33 | 12 | } |
34 | | - |
| 13 | + |
35 | 14 | withName: 'CUSTOM_TX2GENE' { |
36 | 15 | conda = 'python=3.13.0' |
37 | 16 | //conda = 'python=3.9.5' |
38 | 17 | } |
39 | | - |
40 | | - withName: 'FASTP' { |
41 | | - conda = 'bioconda::fastp' |
42 | | - //conda = 'bioconda::fastp=0.23.4' |
43 | | - } |
44 | | - |
45 | | - withName: 'FQ_SUBSAMPLE' { |
46 | | - conda = 'bioconda::fq' |
47 | | - //conda = 'bioconda::fq=0.9.1' |
48 | | - } |
49 | | - |
50 | | - withName: 'GFFREAD' { |
51 | | - conda = 'bioconda::gffread' |
52 | | - //conda = 'bioconda::gffread=0.12.7' |
53 | | - } |
54 | | - |
55 | | - withName: 'GUNZIP' { |
56 | | - conda = 'conda-forge::sed' |
57 | | - //conda = 'conda-forge::grep=3.11 conda-forge::sed=4.8 conda-forge::tar=1.34' |
58 | | - } |
59 | | - |
60 | | - withName: 'HISAT2_ALIGN' { |
61 | | - conda = 'bioconda::hisat2 bioconda::samtools' |
62 | | - //conda = 'bioconda::hisat2=2.2.1 bioconda::samtools=1.16.1' |
63 | | - } |
64 | | - |
65 | | - withName: 'HISAT2_BUILD|HISAT2_EXTRACTSPLICESITES' { |
66 | | - conda = 'bioconda::hisat2' |
67 | | - //conda = 'bioconda::hisat2=2.2.1' |
68 | | - } |
69 | | - |
70 | | - withName: 'KALLISTO_INDEX|KALLISTO_QUANT' { |
71 | | - conda = 'bioconda::kallisto' |
72 | | - //conda = 'bioconda::kallisto=0.48.0' |
73 | | - } |
74 | | - |
75 | | - withName: 'PICARD_MARKDUPLICATES' { |
76 | | - conda = 'bioconda::picard=3.3.0' |
77 | | - //conda = 'bioconda::picard=3.1.1' |
78 | | - } |
79 | | - |
80 | | - withName: 'PRESEQ_LCEXTRAP' { |
81 | | - conda = 'bioconda::preseq' |
82 | | - //conda = 'bioconda::preseq=3.1.2' |
83 | | - } |
84 | | - |
85 | | - withName: 'RSEM_CALCULATEEXPRESSION' { |
86 | | - conda = 'bioconda::rsem bioconda::star' |
87 | | - //conda = 'bioconda::rsem=1.3.3 bioconda::star=2.7.10a' |
88 | | - } |
89 | | - |
90 | | - withName: 'RSEM_PREPAREREFERENCE' { |
91 | | - conda = 'seqera::rsem seqera::star=2.6.1d' |
92 | | - //conda = 'bioconda::rsem=1.3.3 bioconda::star=2.7.10a' |
93 | | - } |
94 | | - |
| 18 | + |
95 | 19 | withName: 'SALMON_INDEX|SALMON_QUANT' { |
96 | 20 | conda = 'bioconda::salmon=1.10.3' |
97 | 21 | //conda = 'bioconda::salmon=1.10.1' |
98 | 22 | } |
99 | | - |
| 23 | + |
100 | 24 | withName: 'SAMTOOLS_FLAGSTAT|SAMTOOLS_IDXSTATS|SAMTOOLS_INDEX|SAMTOOLS_SORT|SAMTOOLS_STATS' { |
101 | 25 | conda = 'bioconda::samtools=1.21 bioconda::htslib' |
102 | 26 | //conda = 'bioconda::samtools=1.20 bioconda::htslib' |
103 | 27 | } |
104 | | - |
105 | | - withName: 'SORTMERNA' { |
106 | | - conda = 'bioconda::sortmerna' |
107 | | - //conda = 'bioconda::sortmerna=4.3.6' |
108 | | - } |
109 | | - |
110 | | - withName: 'STAR_ALIGN|STAR_GENOMEGENERATE' { |
111 | | - conda = 'bioconda::star bioconda::samtools bioconda::htslib conda-forge::gawk' |
112 | | - //conda = 'bioconda::star=2.7.10a bioconda::samtools=1.18 bioconda::htslib=1.18 conda-forge::gawk=5.1.0' |
| 28 | + |
| 29 | + withName: 'TRIMGALORE' { |
| 30 | + conda = 'bioconda::trim-galore=0.6.10 bioconda::cutadapt=4.9' |
| 31 | + //conda = 'bioconda::trim-galore=0.6.7 bioconda::cutadapt=3.4' |
113 | 32 | } |
114 | | - |
| 33 | + |
115 | 34 | withName: 'STRINGTIE_STRINGTIE' { |
116 | 35 | conda = 'bioconda::stringtie=2.2.3' |
117 | 36 | //conda = 'bioconda::stringtie=2.2.1' |
118 | 37 | } |
119 | | - |
| 38 | + |
120 | 39 | withName: 'SUBREAD_FEATURECOUNTS' { |
121 | 40 | conda = 'bioconda::subread=2.0.6' |
122 | 41 | //conda = 'bioconda::subread=2.0.1' |
123 | 42 | } |
124 | | - |
125 | | - withName: 'TRIMGALORE' { |
126 | | - conda = 'bioconda::trim-galore=0.6.10 bioconda::cutadapt=4.9' |
127 | | - //conda = 'bioconda::trim-galore=0.6.7 bioconda::cutadapt=3.4' |
128 | | - } |
129 | 43 |
|
130 | 44 | withName: 'UCSC_BEDGRAPHTOBIGWIG' { |
131 | 45 | conda = 'bioconda::ucsc-bedgraphtobigwig=469' |
132 | 46 | //conda = 'bioconda::ucsc-bedgraphtobigwig=445' |
133 | 47 | } |
134 | | - |
135 | | - withName: 'UMITOOLS_DEDUP|UMITOOLS_EXTRACT|UMITOOLS_PREPAREFORRSEM' { |
136 | | - conda = 'bioconda::umi_tools' |
137 | | - //conda = 'bioconda::umi_tools=1.1.5' |
| 48 | + |
| 49 | + //// Untested, but have aarch conda builds in bioconda/ conda-forge channels. |
| 50 | + //// Should update the nf-core modules |
| 51 | + |
| 52 | + withName: 'BBSPLIT' { |
| 53 | + conda = 'bioconda::bbmap=39.10' |
| 54 | + // conda = 'bioconda::bbmap=39.01' |
| 55 | + } |
| 56 | + |
| 57 | + withName: 'CAT_FASTQ' { |
| 58 | + conda = 'conda-forge::coreutils=9.5' |
| 59 | + //conda = 'conda-forge::coreutils=8.30' |
| 60 | + } |
| 61 | + |
| 62 | + withName: 'CAT_ADDITIONAL_FASTA' { |
| 63 | + conda = 'python=3.13.0' |
| 64 | + //conda = 'conda-forge::coreutils=3.9.5' |
| 65 | + } |
| 66 | + |
| 67 | + withName: 'FQ_SUBSAMPLE' { |
| 68 | + conda = 'bioconda::fq=0.12.0' |
| 69 | + //conda = 'bioconda::fq=0.9.1' |
138 | 70 | } |
| 71 | + |
| 72 | + withName: 'KALLISTO_INDEX|KALLISTO_QUANT' { |
| 73 | + conda = 'bioconda::kallisto=0.51.1' |
| 74 | + //conda = 'bioconda::kallisto=0.48.0' |
| 75 | + } |
| 76 | + |
| 77 | + withName: 'PRESEQ_LCEXTRAP' { |
| 78 | + conda = 'bioconda::preseq=3.2.0' |
| 79 | + //conda = 'bioconda::preseq=3.1.2' |
| 80 | + } |
| 81 | + |
| 82 | + withName: 'SORTMERNA' { |
| 83 | + conda = 'bioconda::sortmerna=4.3.7' |
| 84 | + //conda = 'bioconda::sortmerna=4.3.6' |
| 85 | + } |
| 86 | + |
| 87 | + //// No longer necessary- dev branch version has an aarch 64 build (s) |
| 88 | + |
| 89 | + //withName: 'GUNZIP' { |
| 90 | + // conda = 'conda-forge::sed' |
| 91 | + // //conda = 'conda-forge::grep=3.11 conda-forge::sed=4.8 conda-forge::tar=1.34' |
| 92 | + //} |
| 93 | + |
| 94 | + //withName: 'UNTAR' { |
| 95 | + // conda = 'conda-forge::grep conda-forge::sed conda-forge::tar' |
| 96 | + // //conda = 'conda-forge::grep=3.11 conda-forge::sed=4.8 conda-forge::tar=1.34' |
| 97 | + //} |
| 98 | + |
| 99 | + //withName: 'FASTP' { |
| 100 | + // conda = 'bioconda::fastp' |
| 101 | + // //conda = 'bioconda::fastp=0.23.4' |
| 102 | + //} |
| 103 | + |
| 104 | + //withName: 'GFFREAD' { |
| 105 | + // conda = 'bioconda::gffread' |
| 106 | + // //conda = 'bioconda::gffread=0.12.7' |
| 107 | + //} |
| 108 | + |
| 109 | + //withName: 'UMITOOLS_DEDUP|UMITOOLS_EXTRACT|UMITOOLS_PREPAREFORRSEM' { |
| 110 | + // conda = 'bioconda::umi_tools' |
| 111 | + // //conda = 'bioconda::umi_tools=1.1.5' |
| 112 | + //} |
| 113 | + |
| 114 | + //// No aarch64 builds in bioconda / conda-forge channels |
| 115 | + |
| 116 | + //// a) have seqera aarch64 conda builds |
139 | 117 |
|
140 | | - withName: 'UNTAR' { |
141 | | - conda = 'conda-forge::grep conda-forge::sed conda-forge::tar' |
142 | | - //conda = 'conda-forge::grep=3.11 conda-forge::sed=4.8 conda-forge::tar=1.34' |
| 118 | + withName: '.*:ALIGN_STAR:STAR_ALIGN_IGENOMES' { |
| 119 | + conda = 'seqera::star=2.6.1d bioconda::samtools=1.21 conda-forge::gawk=5.1.0' |
| 120 | + //conda = 'seqera::star=2.6.1d bioconda::samtools=1.10 conda-forge::gawk=5.1.0' |
| 121 | + } |
| 122 | + |
| 123 | + withName: 'STAR_GENOMEGENERATE_IGENOMES' { |
| 124 | + conda = 'seqera::star=2.6.1d bioconda::samtools=1.21 conda-forge::gawk=5.1.0' |
| 125 | + //conda = 'bioconda::star=2.6.1d bioconda::samtools=1.10 conda-forge::gawk=5.1.0' |
143 | 126 | } |
144 | | -} |
| 127 | + |
| 128 | + withName: 'RSEM_PREPAREREFERENCE' { |
| 129 | + conda = 'seqera::rsem=1.3.3 seqera::star=2.6.1d' |
| 130 | + //conda = 'bioconda::rsem=1.3.3 bioconda::star=2.7.10a' |
| 131 | + } |
| 132 | + |
| 133 | + withName: 'RSEM_CALCULATEEXPRESSION' { |
| 134 | + conda = 'seqera::rsem=1.3.3 seqera::star=2.6.1d' |
| 135 | + //conda = 'bioconda::rsem=1.3.3 bioconda::star=2.7.10a' |
| 136 | + } |
| 137 | + |
| 138 | + // NOTE THE OLDER STAR VERSION HERE! |
| 139 | + |
| 140 | + withName: 'STAR_ALIGN|STAR_GENOMEGENERATE' { |
| 141 | + conda = 'seqera::star2.6.1d bioconda::samtools=1.21 bioconda::htslib=1.21 conda-forge::gawk=5.1.0' |
| 142 | + //conda = 'bioconda::star=2.7.10a bioconda::samtools=1.18 bioconda::htslib=1.18 conda-forge::gawk=5.1.0' |
| 143 | + } |
| 144 | + |
| 145 | + //// b) need aarch64 conda builds |
| 146 | + |
| 147 | + //withName: 'HISAT2_ALIGN' { |
| 148 | + // conda = 'bioconda::hisat2 bioconda::samtools' |
| 149 | + // //conda = 'bioconda::hisat2=2.2.1 bioconda::samtools=1.16.1' |
| 150 | + //} |
| 151 | + |
| 152 | + //withName: 'HISAT2_BUILD|HISAT2_EXTRACTSPLICESITES' { |
| 153 | + // conda = 'bioconda::hisat2' |
| 154 | + // //conda = 'bioconda::hisat2=2.2.1' |
| 155 | + //} |
| 156 | + |
| 157 | +} |
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