|
1 | 1 | process { |
2 | 2 |
|
3 | | - // Apply software overrides for ARM compatibility |
| 3 | + //// No aarch64 builds in bioconda / conda-forge channels |
| 4 | + |
| 5 | + //// a) have seqera aarch64 conda builds |
4 | 6 |
|
5 | 7 | withName: '.*:ALIGN_STAR:STAR_ALIGN_IGENOMES' { |
6 | 8 | conda = 'seqera::star=2.6.1d bioconda::samtools=1.21 conda-forge::gawk=5.1.0' |
7 | 9 | //conda = 'seqera::star=2.6.1d bioconda::samtools=1.10 conda-forge::gawk=5.1.0' |
8 | 10 | } |
9 | | - |
| 11 | + |
10 | 12 | withName: 'STAR_GENOMEGENERATE_IGENOMES' { |
11 | | - conda = 'bioconda::star bioconda::samtools=1.21 conda-forge::gawk=5.1.0' |
12 | | - //conda = 'seqera::star=2.6.1d bioconda::samtools=1.10 conda-forge::gawk=5.1.0' |
13 | | - } |
14 | | - |
15 | | - withName: 'BBSPLIT' { |
16 | | - conda = 'bioconda::bbmap' |
17 | | - // conda = 'bioconda::bbmap=39.01' |
18 | | - } |
19 | | - |
20 | | - withName: 'CAT_FASTQ' { |
21 | | - conda = 'conda-forge::coreutils' |
22 | | - //conda = 'conda-forge::coreutils=8.30' |
23 | | - } |
24 | | - |
25 | | - withName: 'CAT_ADDITIONAL_FASTA' { |
26 | | - conda = 'conda-forge::python' |
27 | | - //conda = 'conda-forge::coreutils=3.9.5' |
28 | | - } |
29 | | - |
30 | | - withName: 'CUSTOM_GETCHROMSIZES' { |
31 | | - conda = 'bioconda::samtools=1.21' |
32 | | - //conda = 'bioconda::samtools=1.20 bioconda::htslib=1.20' |
33 | | - } |
34 | | - |
35 | | - withName: 'CUSTOM_TX2GENE' { |
36 | | - conda = 'python=3.13.0' |
37 | | - //conda = 'python=3.9.5' |
38 | | - } |
39 | | - |
40 | | - withName: 'FASTP' { |
41 | | - conda = 'bioconda::fastp' |
42 | | - //conda = 'bioconda::fastp=0.23.4' |
43 | | - } |
44 | | - |
45 | | - withName: 'FQ_SUBSAMPLE' { |
46 | | - conda = 'bioconda::fq' |
47 | | - //conda = 'bioconda::fq=0.9.1' |
48 | | - } |
49 | | - |
50 | | - withName: 'GFFREAD' { |
51 | | - conda = 'bioconda::gffread' |
52 | | - //conda = 'bioconda::gffread=0.12.7' |
53 | | - } |
54 | | - |
55 | | - withName: 'GUNZIP' { |
56 | | - conda = 'conda-forge::sed' |
57 | | - //conda = 'conda-forge::grep=3.11 conda-forge::sed=4.8 conda-forge::tar=1.34' |
58 | | - } |
59 | | - |
60 | | - withName: 'HISAT2_ALIGN' { |
61 | | - conda = 'bioconda::hisat2 bioconda::samtools' |
62 | | - //conda = 'bioconda::hisat2=2.2.1 bioconda::samtools=1.16.1' |
63 | | - } |
64 | | - |
65 | | - withName: 'HISAT2_BUILD|HISAT2_EXTRACTSPLICESITES' { |
66 | | - conda = 'bioconda::hisat2' |
67 | | - //conda = 'bioconda::hisat2=2.2.1' |
68 | | - } |
69 | | - |
70 | | - withName: 'KALLISTO_INDEX|KALLISTO_QUANT' { |
71 | | - conda = 'bioconda::kallisto' |
72 | | - //conda = 'bioconda::kallisto=0.48.0' |
73 | | - } |
74 | | - |
75 | | - withName: 'PICARD_MARKDUPLICATES' { |
76 | | - conda = 'bioconda::picard=3.3.0' |
77 | | - //conda = 'bioconda::picard=3.1.1' |
78 | | - } |
79 | | - |
80 | | - withName: 'PRESEQ_LCEXTRAP' { |
81 | | - conda = 'bioconda::preseq' |
82 | | - //conda = 'bioconda::preseq=3.1.2' |
83 | | - } |
84 | | - |
85 | | - withName: 'RSEM_CALCULATEEXPRESSION' { |
86 | | - conda = 'bioconda::rsem bioconda::star' |
87 | | - //conda = 'bioconda::rsem=1.3.3 bioconda::star=2.7.10a' |
| 13 | + conda = 'seqera::star=2.6.1d bioconda::samtools=1.21 conda-forge::gawk=5.1.0' |
| 14 | + //conda = 'bioconda::star=2.6.1d bioconda::samtools=1.10 conda-forge::gawk=5.1.0' |
88 | 15 | } |
89 | | - |
| 16 | + |
90 | 17 | withName: 'RSEM_PREPAREREFERENCE' { |
91 | | - conda = 'seqera::rsem seqera::star=2.6.1d' |
| 18 | + conda = 'seqera::rsem=1.3.3 seqera::star=2.6.1d' |
92 | 19 | //conda = 'bioconda::rsem=1.3.3 bioconda::star=2.7.10a' |
93 | 20 | } |
94 | | - |
95 | | - withName: 'SALMON_INDEX|SALMON_QUANT' { |
96 | | - conda = 'bioconda::salmon=1.10.3' |
97 | | - //conda = 'bioconda::salmon=1.10.1' |
98 | | - } |
99 | | - |
100 | | - withName: 'SAMTOOLS_FLAGSTAT|SAMTOOLS_IDXSTATS|SAMTOOLS_INDEX|SAMTOOLS_SORT|SAMTOOLS_STATS' { |
101 | | - conda = 'bioconda::samtools=1.21 bioconda::htslib' |
102 | | - //conda = 'bioconda::samtools=1.20 bioconda::htslib' |
103 | | - } |
104 | | - |
105 | | - withName: 'SORTMERNA' { |
106 | | - conda = 'bioconda::sortmerna' |
107 | | - //conda = 'bioconda::sortmerna=4.3.6' |
108 | | - } |
109 | | - |
110 | | - withName: 'STAR_ALIGN|STAR_GENOMEGENERATE' { |
111 | | - conda = 'bioconda::star bioconda::samtools bioconda::htslib conda-forge::gawk' |
112 | | - //conda = 'bioconda::star=2.7.10a bioconda::samtools=1.18 bioconda::htslib=1.18 conda-forge::gawk=5.1.0' |
113 | | - } |
114 | | - |
115 | | - withName: 'STRINGTIE_STRINGTIE' { |
116 | | - conda = 'bioconda::stringtie=2.2.3' |
117 | | - //conda = 'bioconda::stringtie=2.2.1' |
118 | | - } |
119 | | - |
120 | | - withName: 'SUBREAD_FEATURECOUNTS' { |
121 | | - conda = 'bioconda::subread=2.0.6' |
122 | | - //conda = 'bioconda::subread=2.0.1' |
123 | | - } |
124 | | - |
125 | | - withName: 'TRIMGALORE' { |
126 | | - conda = 'bioconda::trim-galore=0.6.10 bioconda::cutadapt=4.9' |
127 | | - //conda = 'bioconda::trim-galore=0.6.7 bioconda::cutadapt=3.4' |
128 | | - } |
129 | 21 |
|
130 | | - withName: 'UCSC_BEDGRAPHTOBIGWIG' { |
131 | | - conda = 'bioconda::ucsc-bedgraphtobigwig=469' |
132 | | - //conda = 'bioconda::ucsc-bedgraphtobigwig=445' |
133 | | - } |
134 | | - |
135 | | - withName: 'UMITOOLS_DEDUP|UMITOOLS_EXTRACT|UMITOOLS_PREPAREFORRSEM' { |
136 | | - conda = 'bioconda::umi_tools' |
137 | | - //conda = 'bioconda::umi_tools=1.1.5' |
138 | | - } |
139 | | - |
140 | | - withName: 'UNTAR' { |
141 | | - conda = 'conda-forge::grep conda-forge::sed conda-forge::tar' |
142 | | - //conda = 'conda-forge::grep=3.11 conda-forge::sed=4.8 conda-forge::tar=1.34' |
| 22 | + withName: 'RSEM_CALCULATEEXPRESSION' { |
| 23 | + conda = 'seqera::rsem=1.3.3 seqera::star=2.6.1d' |
| 24 | + //conda = 'bioconda::rsem=1.3.3 bioconda::star=2.7.10a' |
143 | 25 | } |
144 | | -} |
| 26 | + |
| 27 | + //// b) need aarch64 conda builds |
| 28 | + |
| 29 | + //withName: 'HISAT2_ALIGN' { |
| 30 | + // conda = 'bioconda::hisat2 bioconda::samtools' |
| 31 | + // //conda = 'bioconda::hisat2=2.2.1 bioconda::samtools=1.16.1' |
| 32 | + //} |
| 33 | + |
| 34 | + //withName: 'HISAT2_BUILD|HISAT2_EXTRACTSPLICESITES' { |
| 35 | + // conda = 'bioconda::hisat2' |
| 36 | + // //conda = 'bioconda::hisat2=2.2.1' |
| 37 | + //} |
| 38 | + |
| 39 | +} |
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